Reviewed, UniProtKB/Swiss-Prot P80702 (DIDH_COMTE)

Names and origin

Protein names	Recommended name:     3-alpha-hydroxysteroid dehydrogenase       Short name=3-alpha-HSD     EC=1.1.1.50 Alternative name(s):     Hydroxyprostaglandin dehydrogenase     HSD28
Organism	Comamonas testosteroni (Pseudomonas testosteroni)
Taxonomic identifier	285 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Comamonas

Protein attributes

Sequence length	30 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Along with the 3 alpha-hydroxysteroid dehydrogenase and 3-oxo-reductase activities towards a variety of cis or trans fused A/B ring steroids, it also reduces several xenobiotic carbonyl compounds, including a metyrapone-based class of insecticides, to the respective alcohol metabolites.
Catalytic activity	Androsterone + NAD(P)+ = 5-alpha-androstane-3,17-dione + NAD(P)H.
Subcellular location	Cytoplasm.
Induction	By steroids.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-alpha-hydroxysteroid dehydrogenase (B-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›30	›30	3-alpha-hydroxysteroid dehydrogenase		PRO_0000054656
Regions
	Region	10 – ›30	›21	Involved in cofactor binding By similarity
Sites
	Binding site	29	1	Substrate By similarity
Experimental info
	Non-terminal residue	30	1

Sequences

	Sequence		Length	Mass (Da)
	P80702-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 065E9CF03F1C5A29	FASTA	30	2,829
	10 20 30 DIIVISGAAT GIGAATCKVL EAAGSQIVSI

References

[1]

"Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the Gram-negative bacterium Comamonas testosteroni." Oppermann U.C.T., Maser E. Eur. J. Biochem. 241:744-749(1996) [PubMed: 8944761] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: ATCC 11996 / DSM 50244 / JCM 5832 / NCIB 8955 / NCTC 10698.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1FK8 based on UniProtKB Q9ZFY9.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.50. 4566.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. [Graphical view]
PROSITE	PS00061. ADH_SHORT. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DIDH_COMTE

Accession

Primary (citable) accession number: P80702

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)