Reviewed, UniProtKB/Swiss-Prot P80701 (DIDH_PSESP)

Names and origin

Protein names	Recommended name:     3-alpha-hydroxysteroid dehydrogenase       Short name=3-alpha-HSD     EC=1.1.1.50 Alternative name(s):     Hydroxyprostaglandin dehydrogenase     HSD29
Organism	Pseudomonas sp.
Taxonomic identifier	306 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria

Protein attributes

Sequence length	15 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Along with the 3 alpha-hydroxysteroid dehydrogenase and 3-oxo-reductase activities towards a variety of cis or trans fused A/B ring steroids, it also reduces several xenobiotic carbonyl compounds, including a metyrapone-based class of insecticides, to the respective alcohol metabolites.
Catalytic activity	Androsterone + NAD(P)(+) = 5-alpha-androstane-3,17-dione + NAD(P)H.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-alpha-hydroxysteroid dehydrogenase (B-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›15	›15	3-alpha-hydroxysteroid dehydrogenase		PRO_0000054657
Regions
	Region	6 – ›15	›10	Involved in cofactor binding By similarity
Experimental info
	Non-terminal residue	15	1

Sequences

	Sequence		Length	Mass (Da)
	P80701-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 9506860D070A7790	FASTA	15	1,315
	10 QVIAITGSAS GIGAA

References

[1]	"Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the Gram-negative bacterium Comamonas testosteroni." Oppermann U.C.T., Maser E. Eur. J. Biochem. 241:744-749(1996) [PubMed: 8944761] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. [Graphical view]
PROSITE	PS00061. ADH_SHORT. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DIDH_PSESP

Accession

Primary (citable) accession number: P80701

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 4, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)