Reviewed, UniProtKB/Swiss-Prot P80695 (AMO_KLEOX)

Names and origin

Protein names

Recommended name:
    Primary amine oxidase
    EC=1.4.3.21
Alternative name(s):
    Monamine oxidase
    Tyramine oxidase

Gene names

Name:	maoA
Synonyms:	tynA

Organism

Klebsiella oxytoca

Taxonomic identifier

571 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	56 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Active on tyramine, tryptamine, beta-phenethylamine and dopamine.
Catalytic activity	RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.
Cofactor	Binds 1 copper ion per subunit. Contains 1 topaquinone per subunit By similarity.
Subunit structure	Homodimer.
Subcellular location	Periplasm.
Post-translational modification	Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue By similarity.
Sequence similarities	Belongs to the copper/topaquinone oxidase family.

Ontologies

Keywords
Biological process	Catecholamine metabolism
Cellular component	Periplasm
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	TPQ
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	catecholamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amine oxidase activity Inferred from electronic annotation. Source: EC copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›56	›56	Primary amine oxidase		PRO_0000064108
Experimental info
	Non-adjacent residues	20 – 21	2
	Non-terminal residue	56	1

Sequences

	Sequence		Length	Mass (Da)
	P80695-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 69D96CBC32561840	FASTA	56	6,443
	10 20 30 40 50 HGGEAHMVPM DKTLQDFGAD DKQLWVTRYH PTERFPEGKY PNRSIHDTGL GQYAKD

References

[1]

"Distribution of amine oxidases and amine dehydrogenases in bacteria grown on primary amines and characterization of the amine oxidase from Klebsiella oxytoca." Hacisalihoglu A., Jongejan J.A., Duine J.A. Microbiology 143:505-512(1997) [PubMed: 9043125] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: ATCC 8724 / LMD 72.65.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1OAC based on UniProtKB P46883.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.3.6. 81705.
Family and domain databases
InterPro	IPR000269. Cu_amine_oxidase. IPR015798. Cu_amine_oxidase_C. [Graphical view]
Pfam	PF01179. Cu_amine_oxid. 1 hit. [Graphical view]
PROSITE	PS01164. COPPER_AMINE_OXID_1. Partial match. PS01165. COPPER_AMINE_OXID_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMO_KLEOX

Accession

Primary (citable) accession number: P80695

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)