ID   CUH1C_TENMO             Reviewed;         211 AA.
AC   P80686; O18509;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   05-MAY-2009, entry version 42.
DE   RecName: Full=Larval/pupal cuticle protein H1C;
DE   AltName: Full=TM-PCP H1C;
DE            Short=TM-H1C;
DE   AltName: Full=TMLPCP-22;
DE   Flags: Precursor;
GN   Name=LPCP-22;
OS   Tenebrio molitor (Yellow mealworm).
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia;
OC   Tenebrionidae; Tenebrio.
OX   NCBI_TaxID=7067;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Epidermis;
RX   MEDLINE=96202927; PubMed=8631320;
RX   DOI=10.1111/j.1432-1033.1996.00138.x;
RA   Rondot I., Quennedey B., Courrent A., Lemoine A., Delachambre J.;
RT   "Cloning and sequencing of a cDNA encoding a larval-pupal-specific
RT   cuticular protein in Tenebrio molitor (Insecta, Coleoptera).
RT   Developmental expression and effect of a juvenile hormone analogue.";
RL   Eur. J. Biochem. 235:138-143(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=98321621; PubMed=9660184;
RX   DOI=10.1046/j.1432-1327.1998.2540304.x;
RA   Rondot I., Quennedey B., Delachambre J.;
RT   "Structure, organization and expression of two clustered cuticle
RT   protein genes during the metamorphosis of an insect, Tenebrio
RT   molitor.";
RL   Eur. J. Biochem. 254:304-312(1998).
RN   [3]
RP   PROTEIN SEQUENCE OF 17-211, AND MASS SPECTROMETRY.
RC   TISSUE=Cuticle;
RX   MEDLINE=97218698; PubMed=9066122; DOI=10.1016/S0965-1748(96)00076-8;
RA   Andersen S.O., Rafn K., Roepstorff P.;
RT   "Sequence studies of proteins from larval and pupal cuticle of the
RT   yellow meal worm, Tenebrio molitor.";
RL   Insect Biochem. Mol. Biol. 27:121-131(1997).
CC   -!- FUNCTION: Component of the hard and soft cuticles of the larva and
CC       pupa of Tenebrio molitor.
CC   -!- TISSUE SPECIFICITY: Restricted to sternal, tergal, legs and wing
CC       anlagen epidermis, and soft cuticle secreting epidermis, such as
CC       intersegmental membranes and pleura. No expression in muscles or
CC       fat body.
CC   -!- DEVELOPMENTAL STAGE: Expressed in epidermal cells during the
CC       secretion of preecdysial larval and pupal cuticles. Restricted to
CC       a short part of the intersegmental regions after pupal ecdysis.
CC   -!- INDUCTION: By juvenile hormone (Probable).
CC   -!- DOMAIN: The tetrapeptide (A-A-P-[AV]) repeats found throughout the
CC       protein are also present in many proteins constituting the
CC       protective envelope of other species.
CC   -!- MASS SPECTROMETRY: Mass=19182.7; Method=Electrospray; Range=17-
CC       211; Source=PubMed:9066122;
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DR   EMBL; X87983; CAA61238.1; -; mRNA.
DR   EMBL; Y09124; CAA70342.1; -; Genomic_DNA.
DR   PIR; S62598; S62598.
DR   PIR; T12013; T12013.
DR   GO; GO:0042302; F:structural constituent of cuticle; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cuticle; Direct protein sequencing; Repeat; Signal.
FT   SIGNAL        1     16
FT   CHAIN        17    211       Larval/pupal cuticle protein H1C.
FT                                /FTId=PRO_0000006417.
FT   REPEAT       29     32       1-1.
FT   REPEAT       41     44       1-2.
FT   REPEAT      128    137       2-1.
FT   REPEAT      131    134       1-3.
FT   REPEAT      138    147       2-2.
FT   REPEAT      141    144       1-4.
FT   REPEAT      148    156       3-1.
FT   REPEAT      157    165       3-2.
FT   REPEAT      166    174       3-3.
FT   REPEAT      175    183       3-4.
FT   REGION      128    147       2 X 10 AA tandem repeat of Y-A-H-A-A-P-V-
FT                                V-S-[ST].
FT   REGION      148    183       4 X 9 AA tandem repeat of Y-A-A-H-A-P-V-
FT                                [VA]-[AT].
FT   CONFLICT    156    156       A -> T (in Ref. 3; AA sequence).
FT   CONFLICT    181    181       V -> VATYAAHAPV (in Ref. 1).
SQ   SEQUENCE   211 AA;  20913 MW;  2E21FAE609F4FE37 CRC64;
     MYKFVVFAAA LAYANAGLIG APAVAAYSAA PAVSSAYIHQ AAPVAVAHAA PLAVAHAPVA
     VAHAAPYAIH APAVGASHQS VVRSLGGNQA VSHYSKAVDS AFSSVRKFDT RVTNDALAVA
     HAPVVSTYAH AAPVVSTYAH AAPVVSSYAA HAPVAAYAAH APVATYAAHA PVATYAAHAP
     VVATRTSAVA YSPAAVVSHA SFSGLGASYA W
//