P80686 (CUH1C_TENMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Larval/pupal cuticle protein H1C

Alternative name(s):
TM-PCP H1C
Short name=TM-H1C
TMLPCP-22

Gene names

Name:

LPCP-22

Organism

Tenebrio molitor (Yellow mealworm beetle)

Taxonomic identifier

7067 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Tenebrionidae › Tenebrio

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the hard and soft cuticles of the larva and pupa of Tenebrio molitor.
Tissue specificity	Restricted to sternal, tergal, legs and wing anlagen epidermis, and soft cuticle secreting epidermis, such as intersegmental membranes and pleura. No expression in muscles or fat body.
Developmental stage	Expressed in epidermal cells during the secretion of preecdysial larval and pupal cuticles. Restricted to a short part of the intersegmental regions after pupal ecdysis.
Induction	By juvenile hormone Probable.
Domain	The tetrapeptide (A-A-P-[AV]) repeats found throughout the protein are also present in many proteins constituting the protective envelope of other species.
Mass spectrometry	Molecular mass is 19182.7 Da from positions 17 - 211. Determined by ESI. Ref.3

Ontologies

Keywords
Cellular component	Cuticle
Domain	Repeat Signal
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	structural constituent of cuticle Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Ref.3
	Chain	17 – 211	195	Larval/pupal cuticle protein H1C		PRO_0000006417
Regions
	Repeat	29 – 32	4	1-1
	Repeat	41 – 44	4	1-2
	Repeat	128 – 137	10	2-1
	Repeat	131 – 134	4	1-3
	Repeat	138 – 147	10	2-2
	Repeat	141 – 144	4	1-4
	Repeat	148 – 156	9	3-1
	Repeat	157 – 165	9	3-2
	Repeat	166 – 174	9	3-3
	Repeat	175 – 183	9	3-4
	Region	128 – 147	20	2 X 10 AA tandem repeat of Y-A-H-A-A-P-V-V-S-[ST]
	Region	148 – 183	36	4 X 9 AA tandem repeat of Y-A-A-H-A-P-V-[VA]-[AT]
Experimental info
	Sequence conflict	156	1	A → T AA sequence Ref.3
	Sequence conflict	181	1	V → VATYAAHAPV Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P80686 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: 2E21FAE609F4FE37	FASTA	211	20,913
	10 20 30 40 50 60 MYKFVVFAAA LAYANAGLIG APAVAAYSAA PAVSSAYIHQ AAPVAVAHAA PLAVAHAPVA 70 80 90 100 110 120 VAHAAPYAIH APAVGASHQS VVRSLGGNQA VSHYSKAVDS AFSSVRKFDT RVTNDALAVA 130 140 150 160 170 180 HAPVVSTYAH AAPVVSTYAH AAPVVSSYAA HAPVAAYAAH APVATYAAHA PVATYAAHAP 190 200 210 VVATRTSAVA YSPAAVVSHA SFSGLGASYA W

References

[1]	"Cloning and sequencing of a cDNA encoding a larval-pupal-specific cuticular protein in Tenebrio molitor (Insecta, Coleoptera). Developmental expression and effect of a juvenile hormone analogue." Rondot I., Quennedey B., Courrent A., Lemoine A., Delachambre J. Eur. J. Biochem. 235:138-143(1996) [PubMed: 8631320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Epidermis.
[2]	"Structure, organization and expression of two clustered cuticle protein genes during the metamorphosis of an insect, Tenebrio molitor." Rondot I., Quennedey B., Delachambre J. Eur. J. Biochem. 254:304-312(1998) [PubMed: 9660184] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence studies of proteins from larval and pupal cuticle of the yellow meal worm, Tenebrio molitor." Andersen S.O., Rafn K., Roepstorff P. Insect Biochem. Mol. Biol. 27:121-131(1997) [PubMed: 9066122] [Abstract] Cited for: PROTEIN SEQUENCE OF 17-211, MASS SPECTROMETRY. Tissue: Cuticle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87983 mRNA. Translation: CAA61238.1. Y09124 Genomic DNA. Translation: CAA70342.1.
PIR	S62598. T12013.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022727. Cuticle_C1. [Graphical view]
Pfam	PF11018. Cuticle_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CUH1C_TENMO

Accession

Primary (citable) accession number: P80686
Secondary accession number(s): O18509

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	July 15, 1998
Last modified:	November 30, 2010
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)