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Protein

Gating modifier of anion channels 2

Gene
N/A
Organism
Leiurus quinquestriatus hebraeus (Yellow scorpion)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specific and potent inhibitor of ClC-2/CLCN2 chloride channel. It slows ClC-2/CLCN2 activation by increasing the latency to first opening by nearly 8-fold but is unable to inhibit open channels, suggesting that this toxin inhibits channel activation gating.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel impairing toxin, Ion channel impairing toxin, Toxin, Voltage-gated chloride channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Gating modifier of anion channels 2
Alternative name(s):
Leiuropeptide II
Short name:
LpII
Leiuropeptide-21 Publication
Potassium channel toxin alpha-KTx 8.3
Toxin GaTx21 Publication
OrganismiLeiurus quinquestriatus hebraeus (Yellow scorpion)
Taxonomic identifieri6884 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeLeiurus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Peptidei1 – 2929Gating modifier of anion channels 2PRO_0000044542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi3 ↔ 191 Publication
Disulfide bondi6 ↔ 241 Publication
Disulfide bondi10 ↔ 261 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

3D structure databases

ProteinModelPortaliP80670.
SMRiP80670. Positions 1-29.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR008911. BmTXKS1/BmP02-like.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PfamiPF05453. Toxin_6. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

P80670-1 [UniParc]FASTAAdd to basket

« Hide

        10         20 
VSCEDCPDHC STQKARAKCD NDKCVCEPI
Length:29
Mass (Da):3,199
Last modified:October 1, 1996 - v1
Checksum:iBC5F650C196C15B8
GO

Mass spectrometryi

Molecular mass is 3191.29 Da from positions 1 - 29. Determined by MALDI. 1 Publication

Cross-referencesi

3D structure databases

ProteinModelPortaliP80670.
SMRiP80670. Positions 1-29.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR008911. BmTXKS1/BmP02-like.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PfamiPF05453. Toxin_6. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Characterization of a new family of toxin-like peptides from the venom of the scorpion Leiurus quinquestriatus hebraeus. 1H-NMR structure of leiuropeptide II."
    Buisine E., Wieruszeski J.-M., Lippens G., Wouters D., Tartar A., Sautiere P.
    J. Pept. Res. 49:545-555(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, STRUCTURE BY NMR, DISULFIDE BONDS.
    Tissue: Venom.
  2. "Isolation and characterization of a high affinity peptide inhibitor of ClC-2 chloride channels."
    Thompson C.H., Olivetti P.R., Fuller M.D., Freeman C.S., McMaster D., French R.J., Pohl J., Kubanek J., McCarty N.A.
    J. Biol. Chem. 284:26051-26062(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, SYNTHESIS, FUNCTION, TOXIN TARGET, MASS SPECTROMETRY.
    Tissue: Venom.
  3. "Inhibition of ClC-2 chloride channels by a peptide component or components of scorpion venom."
    Thompson C.H., Fields D.M., Olivetti P.R., Fuller M.D., Zhang Z.R., Kubanek J., McCarty N.A.
    J. Membr. Biol. 208:65-76(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Moving pieces in a taxonomic puzzle: venom 2D-LC/MS and data clustering analyses to infer phylogenetic relationships in some scorpions from the Buthidae family (Scorpiones)."
    Nascimento D.G., Rates B., Santos D.M., Verano-Braga T., Barbosa-Silva A., Dutra A.A.A., Biondi I., Martin-Eauclaire M.-F., De Lima M.E., Pimenta A.M.C.
    Toxicon 47:628-639(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiKAX83_LEIQH
AccessioniPrimary (citable) accession number: P80670
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Does not inhibit ClC-0, ClC-1/CLCN1, ClC-3/CLCN3, ClC-4/CLCN4, CFTR chloride channel, GABRR, Cl(Ca), Shaker, and Kv1.2/KCNA2.

Caution

Has sequence similarities with potassium channel inhibitors, but inhibits chloride channels. It is why the systematic name 'Potassium channel toxin alpha-KTx 8.3' has been moved as alternative name.Curated

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Scorpion potassium channel toxins
    Nomenclature of scorpion potassium channel toxins and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.