Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P80670

- KAX83_LEIQH

UniProt

P80670 - KAX83_LEIQH

Protein

Potassium channel toxin alpha-KTx 8.3

Gene
N/A
Organism
Leiurus quinquestriatus hebraeus (Yellow scorpion)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Specific and potent inhibitor of ClC-2/CLCN2 chloride channel. It slows ClC-2/CLCN2 activation by increasing the latency to first opening by nearly 8-fold but is unable to inhibit open channels, suggesting that this toxin inhibits channel activation gating.2 Publications

    GO - Molecular functioni

    1. chloride channel inhibitor activity Source: UniProtKB-KW

    GO - Biological processi

    1. defense response Source: InterPro
    2. pathogenesis Source: InterPro

    Keywords - Molecular functioni

    Chloride channel impairing toxin, Ion channel impairing toxin, Toxin, Voltage-gated chloride channel impairing toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel toxin alpha-KTx 8.3
    Alternative name(s):
    Gating modifier of anion channels 2
    Leiuropeptide II
    Short name:
    LpII
    Leiuropeptide-2
    Toxin GaTx2
    OrganismiLeiurus quinquestriatus hebraeus (Yellow scorpion)
    Taxonomic identifieri6884 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeLeiurus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Peptidei1 – 2929Potassium channel toxin alpha-KTx 8.3PRO_0000044542Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi3 ↔ 191 Publication
    Disulfide bondi6 ↔ 241 Publication
    Disulfide bondi10 ↔ 261 Publication

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Structurei

    3D structure databases

    ProteinModelPortaliP80670.
    SMRiP80670. Positions 1-29.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR003614. Scorpion_toxin-like.
    IPR008911. Toxin_6.
    [Graphical view]
    PfamiPF05453. Toxin_6. 1 hit.
    [Graphical view]
    SUPFAMiSSF57095. SSF57095. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P80670-1 [UniParc]FASTAAdd to Basket

    « Hide

    VSCEDCPDHC STQKARAKCD NDKCVCEPI                          29
    Length:29
    Mass (Da):3,199
    Last modified:October 1, 1996 - v1
    Checksum:iBC5F650C196C15B8
    GO

    Mass spectrometryi

    Molecular mass is 3191.29 Da from positions 1 - 29. Determined by MALDI. 1 Publication

    Cross-referencesi

    3D structure databases

    ProteinModelPortali P80670.
    SMRi P80670. Positions 1-29.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR003614. Scorpion_toxin-like.
    IPR008911. Toxin_6.
    [Graphical view ]
    Pfami PF05453. Toxin_6. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57095. SSF57095. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a new family of toxin-like peptides from the venom of the scorpion Leiurus quinquestriatus hebraeus. 1H-NMR structure of leiuropeptide II."
      Buisine E., Wieruszeski J.-M., Lippens G., Wouters D., Tartar A., Sautiere P.
      J. Pept. Res. 49:545-555(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, STRUCTURE BY NMR, DISULFIDE BONDS.
      Tissue: Venom.
    2. "Isolation and characterization of a high affinity peptide inhibitor of ClC-2 chloride channels."
      Thompson C.H., Olivetti P.R., Fuller M.D., Freeman C.S., McMaster D., French R.J., Pohl J., Kubanek J., McCarty N.A.
      J. Biol. Chem. 284:26051-26062(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, SYNTHESIS, FUNCTION, TOXIN TARGET, MASS SPECTROMETRY.
      Tissue: Venom.
    3. "Inhibition of ClC-2 chloride channels by a peptide component or components of scorpion venom."
      Thompson C.H., Fields D.M., Olivetti P.R., Fuller M.D., Zhang Z.R., Kubanek J., McCarty N.A.
      J. Membr. Biol. 208:65-76(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Moving pieces in a taxonomic puzzle: venom 2D-LC/MS and data clustering analyses to infer phylogenetic relationships in some scorpions from the Buthidae family (Scorpiones)."
      Nascimento D.G., Rates B., Santos D.M., Verano-Braga T., Barbosa-Silva A., Dutra A.A.A., Biondi I., Martin-Eauclaire M.-F., De Lima M.E., Pimenta A.M.C.
      Toxicon 47:628-639(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiKAX83_LEIQH
    AccessioniPrimary (citable) accession number: P80670
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Miscellaneous

    Does not inhibit ClC-0, ClC-1/CLCN1, ClC-3/CLCN3, ClC-4/CLCN4, CFTR chloride channel, GABRR, Cl(Ca), Shaker, and Kv1.2/KCNA2.

    Caution

    Has sequence similarities with potassium channel inhibitors, but inhibits chloride channels.Curated

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Scorpion potassium channel toxins
      Nomenclature of scorpion potassium channel toxins and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3