Reviewed,
UniProtKB/Swiss-Prot P80668 (FEAB_ECOLI)
Last modified
June 16, 2009.
Version 77.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Phenylacetaldehyde dehydrogenase Short name=PAD EC=1.2.1.39 | ||||||
| Gene names |
| ||||||
| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 499 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Acts almost equally well on phenylacetaldehyde, 4-hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde. |
| Catalytic activity | Phenylacetaldehyde + NAD+ + H2O = phenylacetate + NADH. |
| Pathway | Amino-acid degradation; L-phenylalanine degradation; phenylacetate from L-phenylalanine: step 3/3. |
| Subunit structure | Homodimer. |
| Miscellaneous | Can also use NADP, but prefers NAD. |
| Sequence similarities | Belongs to the aldehyde dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | phenylacetaldehyde dehydrogenase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 499 | 499 | Phenylacetaldehyde dehydrogenase | PRO_0000056575 | |||||
Regions | |||||||||
| Nucleotide binding | 250 – 255 | 6 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 272 | 1 | By similarity | ||||||
| Active site | 306 | 1 | By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 265 | 1 | H → R in CAA66106. Ref.3 | ||||||
| Sequence conflict | 484 – 499 | 16 | DWLDG…VCVRY → PLAGRAGC in CAA67780. Ref.1 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "2-phenylethylamine catabolism by Escherichia coli K-12: gene organization and expression." Hanlon S.P., Hill T.K., Flavell M.A., Stringfellow J.M., Cooper R.A. Microbiology 143:513-518(1997) [PubMed: 9043126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-9. Strain: K12. |
| [2] | Stringfellow J.M. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. |
| [3] | "Molecular characterization of PadA, a phenylacetaldehyde dehydrogenase from Escherichia coli." Ferrandez A., Prieto M.A., Garcia J.L., Diaz E. FEBS Lett. 406:23-27(1997) [PubMed: 9109378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10. Strain: W / ATCC 11105 / DSM 1900. |
| [4] | "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T.DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [6] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [7] | "Nucleotide sequence of the gene for monoamine oxidase (maoA) from Escherichia coli." Azakami H., Yamashita M., Roh J.-H., Suzuki H., Kumagai H., Murooka Y. J. Ferment. Bioeng. 77:315-319(1994) Cited for: PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
Cross-references
Sequence databases | |
|---|---|
| X99402 Genomic DNA. Translation: CAA67780.1. X97453 Genomic DNA. Translation: CAA66106.1. U00096 Genomic DNA. Translation: AAC74467.1. Different initiation. AP009048 Genomic DNA. Translation: BAA14992.1. Different initiation. D23670 Genomic DNA. No translation available. | |
| PIR | D64889. |
| RefSeq | AP_002012.1. NP_415903.4. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1O04 based on UniProtKB P05091. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:9581N. |
Genome annotation databases | |
| GeneID | 945933. |
| GenomeReviews | Gene locus JW1380 in contig AP009048_GR. Gene locus b1385 in contig U00096_GR. |
| KEGG | ecj:JW1380. eco:b1385. |
Organism-specific databases | |
| EchoBASE | EB2971. |
| EcoGene | EG13179. feaB. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P80668. |
| OMA | P80668. SAHHRDK. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:PHENDEHYD-MON. MetaCyc:PHENDEHYD-MON. |
Family and domain databases | |
| InterPro | IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view] |
| Gene3D | G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit. |
| PANTHER | PTHR11699. Aldehyde_dehyd. 1 hit. |
| Pfam | PF00171. Aldedh. 1 hit. [Graphical view] |
| PROSITE | PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | FEAB_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P80668 Secondary accession number(s): O32557, P46884, P77637 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


