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Protein

Phenylacetaldehyde dehydrogenase

Gene

feaB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts almost equally well on phenylacetaldehyde, 4-hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde.

Miscellaneous

Can also use NADP, but prefers NAD.

Catalytic activityi

Phenylacetaldehyde + NAD+ + H2O = phenylacetate + NADH.

Pathwayi: L-phenylalanine degradation

This protein is involved in step 3 of the subpathway that synthesizes phenylacetate from L-phenylalanine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Primary amine oxidase (tynA)
  3. Phenylacetaldehyde dehydrogenase (feaB)
This subpathway is part of the pathway L-phenylalanine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phenylacetate from L-phenylalanine, the pathway L-phenylalanine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei272By similarity1
Active sitei306By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi250 – 255NADBy similarity6

GO - Molecular functioni

  • 4-hydroxyphenylacetaldehyde dehydrogenase activity Source: EcoliWiki
  • oxidoreductase activity Source: EcoliWiki
  • phenylacetaldehyde dehydrogenase activity Source: EcoCyc

GO - Biological processi

  • 4-nitrophenol catabolic process Source: EcoliWiki
  • L-phenylalanine catabolic process Source: UniProtKB-UniPathway
  • NAD biosynthetic process Source: EcoliWiki
  • phenylethylamine catabolic process Source: EcoCyc
  • protein homotetramerization Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:PHENDEHYD-MONOMER
MetaCyc:PHENDEHYD-MONOMER
BRENDAi1.2.1.39 2026
UniPathwayiUPA00139; UER00724

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylacetaldehyde dehydrogenase (EC:1.2.1.39)
Short name:
PAD
Gene namesi
Name:feaB
Synonyms:maoB, padA, ydbG
Ordered Locus Names:b1385, JW1380
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13179 feaB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000565751 – 499Phenylacetaldehyde dehydrogenaseAdd BLAST499

Proteomic databases

EPDiP80668
PaxDbiP80668
PRIDEiP80668

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4261813, 11 interactors
DIPiDIP-9581N
IntActiP80668, 1 interactor
STRINGi316385.ECDH10B_1510

Structurei

3D structure databases

ProteinModelPortaliP80668
SMRiP80668
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105C26 Bacteria
COG1012 LUCA
HOGENOMiHOG000271505
InParanoidiP80668
KOiK00146
OMAiPLSMATW
PhylomeDBiP80668

Family and domain databases

Gene3Di3.40.309.10, 1 hit
3.40.605.10, 2 hits
InterProiView protein in InterPro
IPR016161 Ald_DH/histidinol_DH
IPR016163 Ald_DH_C
IPR016160 Ald_DH_CS_CYS
IPR029510 Ald_DH_CS_GLU
IPR016162 Ald_DH_N
IPR015590 Aldehyde_DH_dom
PfamiView protein in Pfam
PF00171 Aldedh, 1 hit
SUPFAMiSSF53720 SSF53720, 1 hit
PROSITEiView protein in PROSITE
PS00070 ALDEHYDE_DEHYDR_CYS, 1 hit
PS00687 ALDEHYDE_DEHYDR_GLU, 1 hit

Sequencei

Sequence statusi: Complete.

P80668-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEPHVAVLS QVQQFLDRQH GLYIDGRPGP AQSEKRLAIF DPATGQEIAS
60 70 80 90 100
TADANEADVD NAVMSAWRAF VSRRWAGRLP AERERILLRF ADLVEQHSEE
110 120 130 140 150
LAQLETLEQG KSIAISRAFE VGCTLNWMRY TAGLTTKIAG KTLDLSIPLP
160 170 180 190 200
QGARYQAWTR KEPVGVVAGI VPWNFPLMIG MWKVMPALAA GCSIVIKPSE
210 220 230 240 250
TTPLTMLRVA ELASEAGIPD GVFNVVTGSG AVCGAALTSH PHVAKISFTG
260 270 280 290 300
STATGKGIAR TAADHLTRVT LELGGKNPAI VLKDADPQWV IEGLMTGSFL
310 320 330 340 350
NQGQVCAASS RIYIEAPLFD TLVSGFEQAV KSLQVGPGMS PVAQINPLVS
360 370 380 390 400
RAHCDKVCSF LDDAQAQQAE LIRGSNGPAG EGYYVAPTLV VNPDAKLRLT
410 420 430 440 450
REEVFGPVVN LVRVADGEEA LQLANDTEYG LTASVWTQNL SQALEYSDRL
460 470 480 490
QAGTVWVNSH TLIDANLPFG GMKQSGTGRD FGPDWLDGWC ETKSVCVRY
Length:499
Mass (Da):53,699
Last modified:November 1, 1997 - v2
Checksum:i18D7C7D1BE136464
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti265H → R in CAA66106 (PubMed:9109378).Curated1
Sequence conflicti484 – 499DWLDG…VCVRY → PLAGRAGC in CAA67780 (PubMed:9043126).CuratedAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99402 Genomic DNA Translation: CAA67780.1
X97453 Genomic DNA Translation: CAA66106.1
U00096 Genomic DNA Translation: AAC74467.2
AP009048 Genomic DNA Translation: BAA14992.2
D23670 Genomic DNA No translation available.
PIRiD64889
RefSeqiNP_415903.4, NC_000913.3
WP_000138615.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74467; AAC74467; b1385
BAA14992; BAA14992; BAA14992
GeneIDi945933
KEGGiecj:JW1380
eco:b1385
PATRICifig|1411691.4.peg.887

Similar proteinsi

Entry informationi

Entry nameiFEAB_ECOLI
AccessioniPrimary (citable) accession number: P80668
Secondary accession number(s): O32557, P46884, P77637
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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