Reviewed, UniProtKB/Swiss-Prot P80668 (FEAB_ECOLI)

Names and origin

Protein names

Recommended name:
    Phenylacetaldehyde dehydrogenase
      Short name=PAD
    EC=1.2.1.39

Gene names

Name:	feaB
Synonyms:	maoB, padA, ydbG
Ordered Locus Names:	b1385, JW1380

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts almost equally well on phenylacetaldehyde, 4-hydroxyphenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde.
Catalytic activity	Phenylacetaldehyde + NAD+ + H2O = phenylacetate + NADH.
Pathway	Amino-acid degradation; L-phenylalanine degradation; phenylacetate from L-phenylalanine: step 3/3.
Subunit structure	Homodimer.
Miscellaneous	Can also use NADP, but prefers NAD.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phenylacetaldehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Phenylacetaldehyde dehydrogenase		PRO_0000056575
Regions
	Nucleotide binding	250 – 255	6	NAD By similarity
Sites
	Active site	272	1	By similarity
	Active site	306	1	By similarity
Experimental info
	Sequence conflict	265	1	H → R in CAA66106. Ref.3
	Sequence conflict	484 – 499	16	DWLDG…VCVRY → PLAGRAGC in CAA67780. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P80668-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 18D7C7D1BE136464	FASTA	499	53,699
	10 20 30 40 50 60 MTEPHVAVLS QVQQFLDRQH GLYIDGRPGP AQSEKRLAIF DPATGQEIAS TADANEADVD 70 80 90 100 110 120 NAVMSAWRAF VSRRWAGRLP AERERILLRF ADLVEQHSEE LAQLETLEQG KSIAISRAFE 130 140 150 160 170 180 VGCTLNWMRY TAGLTTKIAG KTLDLSIPLP QGARYQAWTR KEPVGVVAGI VPWNFPLMIG 190 200 210 220 230 240 MWKVMPALAA GCSIVIKPSE TTPLTMLRVA ELASEAGIPD GVFNVVTGSG AVCGAALTSH 250 260 270 280 290 300 PHVAKISFTG STATGKGIAR TAADHLTRVT LELGGKNPAI VLKDADPQWV IEGLMTGSFL 310 320 330 340 350 360 NQGQVCAASS RIYIEAPLFD TLVSGFEQAV KSLQVGPGMS PVAQINPLVS RAHCDKVCSF 370 380 390 400 410 420 LDDAQAQQAE LIRGSNGPAG EGYYVAPTLV VNPDAKLRLT REEVFGPVVN LVRVADGEEA 430 440 450 460 470 480 LQLANDTEYG LTASVWTQNL SQALEYSDRL QAGTVWVNSH TLIDANLPFG GMKQSGTGRD 490 FGPDWLDGWC ETKSVCVRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"2-phenylethylamine catabolism by Escherichia coli K-12: gene organization and expression." Hanlon S.P., Hill T.K., Flavell M.A., Stringfellow J.M., Cooper R.A. Microbiology 143:513-518(1997) [PubMed: 9043126] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-9. Strain: K12.
[2]	Stringfellow J.M. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Molecular characterization of PadA, a phenylacetaldehyde dehydrogenase from Escherichia coli." Ferrandez A., Prieto M.A., Garcia J.L., Diaz E. FEBS Lett. 406:23-27(1997) [PubMed: 9109378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10. Strain: W / ATCC 11105 / DSM 1900.
[4]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Nucleotide sequence of the gene for monoamine oxidase (maoA) from Escherichia coli." Azakami H., Yamashita M., Roh J.-H., Suzuki H., Kumagai H., Murooka Y. J. Ferment. Bioeng. 77:315-319(1994) Cited for: PRELIMINARY PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99402 Genomic DNA. Translation: CAA67780.1. X97453 Genomic DNA. Translation: CAA66106.1. U00096 Genomic DNA. Translation: AAC74467.1. Different initiation. AP009048 Genomic DNA. Translation: BAA14992.1. Different initiation. D23670 Genomic DNA. No translation available.
PIR	D64889.
RefSeq	AP_002012.1. NP_415903.4.
3D structure databases
HSSP	HSSP built from PDB template 1O04 based on UniProtKB P05091.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9581N.
Genome annotation databases
GeneID	945933.
GenomeReviews	Gene locus JW1380 in contig AP009048_GR. Gene locus b1385 in contig U00096_GR.
KEGG	ecj:JW1380. eco:b1385.
Organism-specific databases
EchoBASE	EB2971.
EcoGene	EG13179. feaB.
CMR	Search...
Phylogenomic databases
HOGENOM	P80668.
OMA	P80668. SAHHRDK.
Enzyme and pathway databases
BioCyc	EcoCyc:PHENDEHYD-MON. MetaCyc:PHENDEHYD-MON.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FEAB_ECOLI

Accession

Primary (citable) accession number: P80668
Secondary accession number(s): O32557, P46884, P77637

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)