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Protein

Peroxisomal membrane protein PAS20

Gene

PEX13

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the peroxisomal translocation machinery with PEX14 and PEX17. Interacts with the PTS1 receptor (PAS10/PEX5).

GO - Molecular functioni

  1. protein binding, bridging Source: SGD

GO - Biological processi

  1. protein import into peroxisome matrix, docking Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32313-MONOMER.

Protein family/group databases

TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal membrane protein PAS20
Alternative name(s):
Peroxin-13
Gene namesi
Name:PEX13
Synonyms:PAS20
Ordered Locus Names:YLR191W
ORF Names:L9470.1
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR191w.
SGDiS000004181. PEX13.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 263263LumenalSequence AnalysisAdd
BLAST
Transmembranei264 – 28017HelicalSequence AnalysisAdd
BLAST
Topological domaini281 – 386106CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. peroxisomal importomer complex Source: SGD
  3. peroxisomal membrane Source: SGD
  4. Pex17p-Pex14p docking complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Peroxisomal membrane protein PAS20PRO_0000058324Add
BLAST

Proteomic databases

MaxQBiP80667.
PaxDbiP80667.

Expressioni

Gene expression databases

GenevestigatoriP80667.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
APP1P539332EBI-13206,EBI-28798
INN1P539012EBI-13206,EBI-28955
LAS17Q124462EBI-13206,EBI-10022
PEX10Q055687EBI-13206,EBI-13194
PEX12Q043706EBI-13206,EBI-2077297
PEX14P5311217EBI-13206,EBI-13212
PEX2P3280010EBI-13206,EBI-13160
PEX5P350566EBI-13206,EBI-13170
PRK1P404942EBI-13206,EBI-9703
STE20Q034973EBI-13206,EBI-18285
STP22P256042EBI-13206,EBI-411625
UBP7P404532EBI-13206,EBI-19857

Protein-protein interaction databases

BioGridi31461. 124 interactions.
DIPiDIP-2473N.
IntActiP80667. 79 interactions.
MINTiMINT-433671.
STRINGi4932.YLR191W.

Structurei

Secondary structure

1
386
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi305 – 3073Combined sources
Beta strandi309 – 3157Combined sources
Turni322 – 3243Combined sources
Beta strandi333 – 3408Combined sources
Beta strandi346 – 3538Combined sources
Turni355 – 3573Combined sources
Beta strandi359 – 3635Combined sources
Helixi364 – 3663Combined sources
Beta strandi367 – 3693Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JQQX-ray2.65A/B/C/D299-386[»]
1N5ZX-ray2.70A/B299-386[»]
1NM7NMR-A310-370[»]
2V1RX-ray2.10A/B299-374[»]
ProteinModelPortaliP80667.
SMRiP80667. Positions 299-376.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80667.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini306 – 37267SH3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peroxin-13 family.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG281515.
GeneTreeiENSGT00390000016883.
HOGENOMiHOG000158061.
InParanoidiP80667.
KOiK13344.
OMAiNRTASNY.
OrthoDBiEOG7XDBSF.

Family and domain databases

InterProiIPR007223. Peroxin-13_N.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF04088. Peroxin-13_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P80667-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTAVPRPK PWETSASLEE PQRNAQSLSA MMTSNQQDSR PTEESNNSNS
60 70 80 90 100
ASESAPEVLP RPAALNSSGT YGESNTIPGI YGNSNYGIPY DNNPYSMNSI
110 120 130 140 150
YGNSIGRYGY GGSYYGNNYG SFYGGGYGAG AGYGMNNGSG LGESTKATFQ
160 170 180 190 200
LIESLIGAVT GFAQMLESTY MATHNSFFTM ISVAEQFGNL KEMLGSFFGI
210 220 230 240 250
FAIMKFLKKI LYRATKGRLG IPPKNFAESE GSKNKLIEDF QKFNDSGTIN
260 270 280 290 300
SNEKATRRKI SWKPLLFFLM AVFGFPYLLN KFITKLQTSG TIRASQGNGS
310 320 330 340 350
EPIDPSKLEF ARALYDFVPE NPEMEVALKK GDLMAILSKK DPLGRDSDWW
360 370 380
KVRTKNGNIG YIPYNYIEII KRRKKIEHVD DETRTH
Length:386
Mass (Da):42,706
Last modified:October 1, 1996 - v1
Checksum:iDBEA9A2372185860
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82971 Genomic DNA. Translation: AAB46885.1.
U37420 Genomic DNA. Translation: AAA79308.1.
U17246 Genomic DNA. Translation: AAB67453.1.
U14913 Genomic DNA. Translation: AAB67448.1.
BK006945 Genomic DNA. Translation: DAA09510.1.
PIRiS51436.
RefSeqiNP_013292.1. NM_001182078.1.

Genome annotation databases

EnsemblFungiiYLR191W; YLR191W; YLR191W.
GeneIDi850888.
KEGGisce:YLR191W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82971 Genomic DNA. Translation: AAB46885.1.
U37420 Genomic DNA. Translation: AAA79308.1.
U17246 Genomic DNA. Translation: AAB67453.1.
U14913 Genomic DNA. Translation: AAB67448.1.
BK006945 Genomic DNA. Translation: DAA09510.1.
PIRiS51436.
RefSeqiNP_013292.1. NM_001182078.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JQQX-ray2.65A/B/C/D299-386[»]
1N5ZX-ray2.70A/B299-386[»]
1NM7NMR-A310-370[»]
2V1RX-ray2.10A/B299-374[»]
ProteinModelPortaliP80667.
SMRiP80667. Positions 299-376.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31461. 124 interactions.
DIPiDIP-2473N.
IntActiP80667. 79 interactions.
MINTiMINT-433671.
STRINGi4932.YLR191W.

Protein family/group databases

TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Proteomic databases

MaxQBiP80667.
PaxDbiP80667.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR191W; YLR191W; YLR191W.
GeneIDi850888.
KEGGisce:YLR191W.

Organism-specific databases

CYGDiYLR191w.
SGDiS000004181. PEX13.

Phylogenomic databases

eggNOGiNOG281515.
GeneTreeiENSGT00390000016883.
HOGENOMiHOG000158061.
InParanoidiP80667.
KOiK13344.
OMAiNRTASNY.
OrthoDBiEOG7XDBSF.

Enzyme and pathway databases

BioCyciYEAST:G3O-32313-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP80667.
NextBioi967254.
PROiP80667.

Gene expression databases

GenevestigatoriP80667.

Family and domain databases

InterProiIPR007223. Peroxin-13_N.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF04088. Peroxin-13_N. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The SH3 domain of the Saccharomyces cerevisiae peroxisomal membrane protein Pex13p functions as a docking site for Pex5p, a mobile receptor for the import PTS1-containing proteins."
    Elgersma Y., Kwast L., Klein A., Voorn-Brouwer T., van den Berg M., Tabak H.F., Distel B.
    J. Cell Biol. 135:97-109(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Identification of Pex13p a peroxisomal membrane receptor for the PTS1 recognition factor."
    Erdmann R., Blobel G.
    J. Cell Biol. 135:111-121(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPEX13_YEAST
AccessioniPrimary (citable) accession number: P80667
Secondary accession number(s): D6VYJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.