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Protein

Chymotrypsin B

Gene
N/A
Organism
Gadus morhua (Atlantic cod)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei57 – 571Charge relay systemBy similarity
Active sitei101 – 1011Charge relay systemBy similarity
Active sitei195 – 1951Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Protein family/group databases

MEROPSiS01.437.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin B (EC:3.4.21.1)
Cleaved into the following 2 chains:
OrganismiGadus morhua (Atlantic cod)
Taxonomic identifieri8049 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataZeiogadariaGadariaeGadiformesGadoideiGadidaeGadus

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1313Chymotrypsin B chain APRO_0000027647Add
BLAST
Propeptidei14 – 1521 PublicationPRO_0000027648
Chaini16 – 245230Chymotrypsin B chain BPRO_0000027649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi1 ↔ 121PROSITE-ProRule annotation
Disulfide bondi42 ↔ 58PROSITE-ProRule annotation
Disulfide bondi135 ↔ 201PROSITE-ProRule annotation
Disulfide bondi167 ↔ 182PROSITE-ProRule annotation
Disulfide bondi191 ↔ 220PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP80646.
SMRiP80646. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 243228Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG013304.
OrthoDBiEOG7MKW6Q.
TreeFamiTF330455.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80646-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
CGSPAIQPQV TGYARIVNGE EAVPHSWPWQ VSLQQSNGFH FCGGSLINEN
60 70 80 90 100
WVVTAAHCNV RTYHRVIVGE HDKASDENIQ ILKPSMVFTH PKWDSRTINN
110 120 130 140 150
DISLIKLASP AVLGTNVSPV CLGESSDVFA PGMKCVTSGW GLTRYNAPGT
160 170 180 190 200
PNKLQQAALP LMSNEECSQT WGNNMISDVM ICAGAAGATS CMGDSGGPLV
210 220 230 240
CQKDNVWTLV GIVSWGSSRC SVTTPAVYAR VTELRGWVDQ ILAAN
Length:245
Mass (Da):26,260
Last modified:October 1, 1996 - v1
Checksum:i74FE0D425517AB02
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 113QVT → VIS AA sequence (PubMed:1764912)Curated
Sequence conflicti26 – 261S → T AA sequence (PubMed:1764912)Curated
Sequence conflicti28 – 292PW → Y AA sequence (PubMed:1764912)Curated

Genome annotation databases

EnsembliENSGMOT00000010525; ENSGMOP00000010246; ENSGMOG00000009511.
ENSGMOT00000010541; ENSGMOP00000010261; ENSGMOG00000009511.

Cross-referencesi

3D structure databases

ProteinModelPortaliP80646.
SMRiP80646. Positions 1-245.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.437.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGMOT00000010525; ENSGMOP00000010246; ENSGMOG00000009511.
ENSGMOT00000010541; ENSGMOP00000010261; ENSGMOG00000009511.

Phylogenomic databases

HOVERGENiHBG013304.
OrthoDBiEOG7MKW6Q.
TreeFamiTF330455.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: PROTEIN SEQUENCE.
    Tissue: Pyloric caecum.
  2. "Structural and kinetic properties of chymotrypsin from Atlantic cod (Gadus morhua). Comparison with bovine chymotrypsin."
    Asgeirsson B., Bjarnason J.B.
    Comp. Biochem. Physiol. 99B:327-335(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-12 AND 16-31.
    Tissue: Pyloric caecum.

Entry informationi

Entry nameiCTRB_GADMO
AccessioniPrimary (citable) accession number: P80646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.