Reviewed, UniProtKB/Swiss-Prot P80646 (CTRB_GADMO)

Names and origin

Protein names	Recommended name:     Chymotrypsin B     EC=3.4.21.1 Cleaved into the following 2 chains:     1- Recommended name:             Chymotrypsin B chain A     2- Recommended name:             Chymotrypsin B chain B
Organism	Gadus morhua (Atlantic cod)
Taxonomic identifier	8049 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Paracanthopterygii › Gadiformes › Gadidae › Gadus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 13	13	Chymotrypsin B chain A		PRO_0000027647
	Propeptide	14 – 15	2			PRO_0000027648
	Chain	16 – 245	230	Chymotrypsin B chain B		PRO_0000027649
Regions
	Domain	16 – 243	228	Peptidase S1
Sites
	Active site	57	1	Charge relay system By similarity
	Active site	101	1	Charge relay system By similarity
	Active site	195	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	1 ↔ 121		By similarity
	Disulfide bond	42 ↔ 58		By similarity
	Disulfide bond	135 ↔ 201		By similarity
	Disulfide bond	167 ↔ 182		By similarity
	Disulfide bond	191 ↔ 220		By similarity
Experimental info
	Sequence conflict	9 – 11	3	QVT → VIS AA sequence Ref.2
	Sequence conflict	26	1	S → T AA sequence Ref.2
	Sequence conflict	28 – 29	2	PW → Y AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P80646-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 74FE0D425517AB02	FASTA	245	26,260
	10 20 30 40 50 60 CGSPAIQPQV TGYARIVNGE EAVPHSWPWQ VSLQQSNGFH FCGGSLINEN WVVTAAHCNV 70 80 90 100 110 120 RTYHRVIVGE HDKASDENIQ ILKPSMVFTH PKWDSRTINN DISLIKLASP AVLGTNVSPV 130 140 150 160 170 180 CLGESSDVFA PGMKCVTSGW GLTRYNAPGT PNKLQQAALP LMSNEECSQT WGNNMISDVM 190 200 210 220 230 240 ICAGAAGATS CMGDSGGPLV CQKDNVWTLV GIVSWGSSRC SVTTPAVYAR VTELRGWVDQ ILAAN

References

[1]	"Structure of chymotrypsin variant B from Atlantic cod, Gadus morhua." Leth-Larsen R., Asgeirsson B., Thorolfsson M., Noerregaard-Madsen M., Hoejrup P. Biochim. Biophys. Acta 1297:49-56(1996) [PubMed: 8841380] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Pyloric caecum.
[2]	"Structural and kinetic properties of chymotrypsin from Atlantic cod (Gadus morhua). Comparison with bovine chymotrypsin." Asgeirsson B., Bjarnason J.B. Comp. Biochem. Physiol. 99B:327-335(1991) [PubMed: 1764912] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12 AND 16-31. Tissue: Pyloric caecum.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1CHG based on UniProtKB P00766.
ModBase	Search...
Protein family/group databases
MEROPS	S01.437.
Phylogenomic databases
HOVERGEN	P80646.
Enzyme and pathway databases
BRENDA	3.4.21.1. 39336.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTRB_GADMO

Accession

Primary (citable) accession number: P80646

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)