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Protein

Alkanesulfonate monooxygenase

Gene

ssuD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in desulfonation of aliphatic sulfonates. Catalyzes the conversion of pentanesulfonic acid to sulfite and pentaldehyde and is able to desulfonate a wide range of sulfonated substrates including C-2 to C-10 unsubstituted linear alkanesulfonates, substituted ethanesulfonic acids and sulfonated buffers.

Miscellaneous

FMNH2 which is absolutely required for this enzymatic reaction, is provided by SsuE.

Catalytic activityi

An alkanesulfonate (R-CH2-SO3H) + FMNH2 + O2 = an aldehyde (R-CHO) + FMN + sulfite + H2O.

GO - Molecular functioni

  • alkanesulfonate monooxygenase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc

GO - Biological processi

  • alkanesulfonate catabolic process Source: EcoCyc
  • response to heat Source: EcoCyc

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandFlavoprotein, FMN

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER-162
MetaCyc:MONOMER-162

Names & Taxonomyi

Protein namesi
Recommended name:
Alkanesulfonate monooxygenase (EC:1.14.14.5)
Alternative name(s):
FMNH2-dependent aliphatic sulfonate monooxygenase
Sulfate starvation-induced protein 6
Short name:
SSI6
Gene namesi
Name:ssuD
Synonyms:ycbN
Ordered Locus Names:b0935, JW0918
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13706 ssuD

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi298R → C: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002167062 – 381Alkanesulfonate monooxygenaseAdd BLAST380

Proteomic databases

PaxDbiP80645
PRIDEiP80645

Expressioni

Inductioni

Repressed by sulfate or cysteine.

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
ssuEP806442EBI-561637,EBI-1121047

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4260022, 16 interactors
DIPiDIP-10928N
IntActiP80645, 10 interactors
STRINGi316385.ECDH10B_1005

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Beta strandi14 – 17Combined sources4
Helixi29 – 42Combined sources14
Beta strandi45 – 49Combined sources5
Helixi58 – 63Combined sources6
Beta strandi73 – 78Combined sources6
Turni80 – 82Combined sources3
Helixi85 – 98Combined sources14
Beta strandi103 – 107Combined sources5
Helixi113 – 119Combined sources7
Helixi127 – 142Combined sources16
Turni143 – 145Combined sources3
Beta strandi148 – 151Combined sources4
Beta strandi156 – 160Combined sources5
Beta strandi167 – 172Combined sources6
Beta strandi174 – 177Combined sources4
Helixi181 – 190Combined sources10
Beta strandi192 – 197Combined sources6
Helixi201 – 216Combined sources16
Turni217 – 219Combined sources3
Beta strandi223 – 235Combined sources13
Helixi236 – 246Combined sources11
Turni247 – 249Combined sources3
Helixi252 – 261Combined sources10
Beta strandi285 – 287Combined sources3
Helixi292 – 295Combined sources4
Beta strandi296 – 300Combined sources5
Beta strandi304 – 308Combined sources5
Helixi309 – 320Combined sources12
Turni321 – 323Combined sources3
Beta strandi324 – 330Combined sources7
Helixi334 – 344Combined sources11
Helixi346 – 348Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M41X-ray2.30A/B2-381[»]
1NQKX-ray2.20A1-381[»]
ProteinModelPortaliP80645
SMRiP80645
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80645

Family & Domainsi

Sequence similaritiesi

Belongs to the SsuD family.Curated

Phylogenomic databases

eggNOGiENOG4105D4R Bacteria
COG2141 LUCA
HOGENOMiHOG000134568
InParanoidiP80645
KOiK04091
OMAiNIFWFLP
PhylomeDBiP80645

Family and domain databases

Gene3Di3.20.20.30, 1 hit
HAMAPiMF_01229 Alkanesulf_monooxygen, 1 hit
InterProiView protein in InterPro
IPR019911 Alkanesulphonate_mOase_FMN-dep
IPR011251 Luciferase-like_dom
IPR036661 Luciferase-like_sf
PfamiView protein in Pfam
PF00296 Bac_luciferase, 1 hit
SUPFAMiSSF51679 SSF51679, 1 hit
TIGRFAMsiTIGR03565 alk_sulf_monoox, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLNMFWFLP THGDGHYLGT EEGSRPVDHG YLQQIAQAAD RLGYTGVLIP
60 70 80 90 100
TGRSCEDAWL VAASMIPVTQ RLKFLVALRP SVTSPTVAAR QAATLDRLSN
110 120 130 140 150
GRALFNLVTG SDPQELAGDG VFLDHSERYE ASAEFTQVWR RLLQRETVDF
160 170 180 190 200
NGKHIHVRGA KLLFPAIQQP YPPLYFGGSS DVAQELAAEQ VDLYLTWGEP
210 220 230 240 250
PELVKEKIEQ VRAKAAAHGR KIRFGIRLHV IVRETNDEAW QAAERLISHL
260 270 280 290 300
DDETIAKAQA AFARTDSVGQ QRMAALHNGK RDNLEISPNL WAGVGLVRGG
310 320 330 340 350
AGTALVGDGP TVAARINEYA ALGIDSFVLS GYPHLEEAYR VGELLFPLLD
360 370 380
VAIPEIPQPQ PLNPQGEAVA NDFIPRKVAQ S
Length:381
Mass (Da):41,736
Last modified:January 23, 2007 - v3
Checksum:i4D05E510487999C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19G → Q AA sequence (PubMed:8774726).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti298R → C in strain: K12 / MC4100; inactive. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237695 Genomic DNA Translation: CAB40391.1
U00096 Genomic DNA Translation: AAC74021.1
AP009048 Genomic DNA Translation: BAA35690.1
PIRiF64833
RefSeqiNP_415455.1, NC_000913.3
WP_000056006.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74021; AAC74021; b0935
BAA35690; BAA35690; BAA35690
GeneIDi945557
KEGGiecj:JW0918
eco:b0935
PATRICifig|1411691.4.peg.1339

Similar proteinsi

Entry informationi

Entry nameiSSUD_ECOLI
AccessioniPrimary (citable) accession number: P80645
Secondary accession number(s): P75852, Q9RM46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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