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P80607

- UPTG_MAIZE

UniProt

P80607 - UPTG_MAIZE

Protein

Alpha-1,4-glucan-protein synthase [UDP-forming]

Gene

UPTG

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (09 Sep 2003)
      Previous versions | rss
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    Functioni

    Possible role in the synthesis of cell wall polysaccharides.By similarity

    Catalytic activityi

    UDP-glucose + protein = UDP + alpha-D-glucosyl-protein.1 Publication

    Enzyme regulationi

    Inhibited by inhibitor protein (IP) which may be a form of sucrose synthase.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei158 – 1581Required for activityBy similarity
    Sitei165 – 1651Required for activityBy similarity

    GO - Molecular functioni

    1. intramolecular transferase activity Source: InterPro
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

    GO - Biological processi

    1. cellulose biosynthetic process Source: UniProtKB-KW
    2. plant-type cell wall organization or biogenesis Source: EnsemblPlants/Gramene
    3. pollen development Source: EnsemblPlants/Gramene
    4. response to cadmium ion Source: EnsemblPlants/Gramene
    5. response to salt stress Source: EnsemblPlants/Gramene
    6. UDP-L-arabinose metabolic process Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Cellulose biosynthesis

    Protein family/group databases

    CAZyiGT75. Glycosyltransferase Family 75.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-1,4-glucan-protein synthase [UDP-forming] (EC:2.4.1.-)
    Alternative name(s):
    Amylogenin
    Golgi-associated protein se-wap41
    Reversibly glycosylated polypeptide
    Short name:
    RGP
    UDP-glucose:protein transglucosylase
    Short name:
    UPTG
    Gene namesi
    Name:UPTG
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiP80607.
    MaizeGDBi123920.
    131466.

    Subcellular locationi

    Secretedcell wall 1 Publication. Cell junctionplasmodesma 1 Publication. Golgi apparatus 1 Publication
    Note: Cell wall-associated, with highest concentrations on plasmodesmata. Also located in the Golgi apparatus.

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. cytosolic ribosome Source: EnsemblPlants/Gramene
    3. extracellular region Source: UniProtKB-KW
    4. Golgi trans cisterna Source: EnsemblPlants/Gramene
    5. plasmodesma Source: UniProtKB-SubCell
    6. vacuolar membrane Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Cell junction, Cell wall, Golgi apparatus, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 364364Alpha-1,4-glucan-protein synthase [UDP-forming]PRO_0000221193Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi158 – 1581N-linked (Glc...)1 Publication

    Post-translational modificationi

    Reversibly glycosylated by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP80607.
    ProMEXiP80607.

    Interactioni

    Subunit structurei

    Homopentamer or homohexamer.By similarity

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi110 – 1123DXD motif

    Domaini

    The conserved DXD motif is involved in enzyme activity.By similarity

    Sequence similaritiesi

    Belongs to the RGP family.Curated

    Phylogenomic databases

    HOGENOMiHOG000234443.
    KOiK13379.

    Family and domain databases

    InterProiIPR029044. Nucleotide-diphossugar_trans.
    IPR004901. RGP_fam.
    [Graphical view]
    PfamiPF03214. RGP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016429. UPTG. 1 hit.
    SUPFAMiSSF53448. SSF53448. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P80607-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGTVTVPGS STPSTPLLKD ELDIVIPTIR NLDFLEMWRA FFQPYHLIIV    50
    QDGDPTKTIK VPEGFDYELY NRNDINRILG PKASCISFKD SACRCFGYMV 100
    SKKKYIYTID DDCFVAKDPS GKDINALEQH IKNLLSPSTP FFFNTLYDPY 150
    REGADFVRGY PFSLREGAHT AVSHGLWLNI PDYDAPTQLV KPKERNERYV 200
    DAVMTIPKGT LFPMCGMNLA FDRDLIGPAM YFGLMGDGQP IGRYDDMWAG 250
    WCVKVICDHL SLGVKTGLPY IWHSKASNPF VNLKKEYKGI FWQEDIIPFF 300
    QNVTIPKDCD TVQKCYIYLS GQVKEKLGTI DPYFVKLGDA MVTWIEAWDE 350
    LNPSTPAAAN GKAK 364
    Length:364
    Mass (Da):41,204
    Last modified:September 9, 2003 - v2
    Checksum:i00CEC4FD30378D0C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti40 – 401A → P AA sequence (PubMed:8521968)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U89897 mRNA. Translation: AAB49896.1.
    PIRiT04331.
    RefSeqiNP_001105598.1. NM_001112128.1.
    UniGeneiZm.752.

    Genome annotation databases

    GeneIDi542592.
    KEGGizma:542592.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U89897 mRNA. Translation: AAB49896.1 .
    PIRi T04331.
    RefSeqi NP_001105598.1. NM_001112128.1.
    UniGenei Zm.752.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT75. Glycosyltransferase Family 75.

    Proteomic databases

    PRIDEi P80607.
    ProMEXi P80607.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 542592.
    KEGGi zma:542592.

    Organism-specific databases

    Gramenei P80607.
    MaizeGDBi 123920.
    131466.

    Phylogenomic databases

    HOGENOMi HOG000234443.
    KOi K13379.

    Family and domain databases

    InterProi IPR029044. Nucleotide-diphossugar_trans.
    IPR004901. RGP_fam.
    [Graphical view ]
    Pfami PF03214. RGP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016429. UPTG. 1 hit.
    SUPFAMi SSF53448. SSF53448. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a maize 41 kDa Golgi associated protein."
      Katz A., Van Lent J.W.M., Kotlizky G., Yahalom A., Epel B.L.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Jubile.
    2. "Beta-glucosylarginine: a new glucose-protein bond in a self-glucosylating protein from sweet corn."
      Singh D.G., Lomako J., Lomako W.M., Whelan W.J., Meyer H.E., Serwe M., Metzger J.W.
      FEBS Lett. 376:61-64(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 31-57; 133-193; 199-208; 244-254; 289-307 AND 337-359, GLYCOSYLATION.
    3. "The maize two dimensional gel protein database: towards an integrated genome analysis program."
      Touzet P., Riccardi F., Morin C., Damerval C., Huet J.-C., Pernollet J.-C., Zivy M., de Vienne D.
      Theor. Appl. Genet. 93:997-1005(1996)
      [AGRICOLA] [Europe PMC]
      Cited for: PROTEIN SEQUENCE OF 61-75.
      Tissue: Coleoptile.
    4. "UDP-glucose:protein transglucosylase in developing maize endosperm."
      Rothschild A., Tandecarz J.S.
      Plant Sci. 97:119-127(1994)
      Cited for: ENZYME ACTIVITY.
    5. "A 41kDa protein isolated from maize mesocotyl cell walls immunolocalizes to plasmodesmata."
      Epel B.L., Van Lent J.W.M., Cohen L., Kotlizky G., Katz A., Yahalom A.
      Protoplasma 191:70-78(1996)
      Cited for: SUBCELLULAR LOCATION.
    6. "Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor."
      Rothschild A., Wald F.A., Bocca S.N., Tandecarz J.S.
      Cell. Mol. Biol. 42:645-651(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INHIBITION BY IP.
    7. "Identification of a UPTG inhibitor protein from maize endosperm: high homology with sucrose synthase protein."
      Wald F.A., Rothschild A., Moreno S., Tandecarz J.S.
      Cell. Mol. Biol. 44:397-406(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INHIBITION BY IP.

    Entry informationi

    Entry nameiUPTG_MAIZE
    AccessioniPrimary (citable) accession number: P80607
    Secondary accession number(s): Q9SAQ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 9, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3