P80607 (UPTG_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,4-glucan-protein synthase [UDP-forming]
EC=2.4.1.-

Alternative name(s):
Amylogenin
Golgi-associated protein se-wap41
Reversibly glycosylated polypeptide
Short name=RGP
UDP-glucose:protein transglucosylase
Short name=UPTG

Gene names

Name:

UPTG

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possible role in the synthesis of cell wall polysaccharides By similarity.
Catalytic activity	UDP-glucose + protein = UDP + alpha-D-glucosyl-protein. Ref.4
Enzyme regulation	Inhibited by inhibitor protein (IP) which may be a form of sucrose synthase.
Subunit structure	Homopentamer or homohexamer By similarity.
Subcellular location	Secreted › cell wall. Cell junction › plasmodesma. Golgi apparatus. Note: Cell wall-associated, with highest concentrations on plasmodesmata. Also located in the Golgi apparatus. Ref.5
Domain	The conserved DXD motif is involved in enzyme activity By similarity.
Post-translational modification	Reversibly glycosylated by UDP-glucose, UDP-xylose and UDP-galactose, but not UDP-mannose. Ref.2
Sequence similarities	Belongs to the RGP family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Cellulose biosynthesis
Cellular component	Cell junction Cell wall Golgi apparatus Secreted
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW plasmodesma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycogenin glucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Alpha-1,4-glucan-protein synthase [UDP-forming]		PRO_0000221193
Regions
	Motif	110 – 112	3	DXD motif
Sites
	Site	158	1	Required for activity By similarity
	Site	165	1	Required for activity By similarity
Amino acid modifications
	Glycosylation	158	1	N-linked (Glc...)
Experimental info
	Sequence conflict	40	1	A → P AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P80607 [UniParc]. Last modified September 9, 2003. Version 2. Checksum: 00CEC4FD30378D0C	FASTA	364	41,204
	10 20 30 40 50 60 MAGTVTVPGS STPSTPLLKD ELDIVIPTIR NLDFLEMWRA FFQPYHLIIV QDGDPTKTIK 70 80 90 100 110 120 VPEGFDYELY NRNDINRILG PKASCISFKD SACRCFGYMV SKKKYIYTID DDCFVAKDPS 130 140 150 160 170 180 GKDINALEQH IKNLLSPSTP FFFNTLYDPY REGADFVRGY PFSLREGAHT AVSHGLWLNI 190 200 210 220 230 240 PDYDAPTQLV KPKERNERYV DAVMTIPKGT LFPMCGMNLA FDRDLIGPAM YFGLMGDGQP 250 260 270 280 290 300 IGRYDDMWAG WCVKVICDHL SLGVKTGLPY IWHSKASNPF VNLKKEYKGI FWQEDIIPFF 310 320 330 340 350 360 QNVTIPKDCD TVQKCYIYLS GQVKEKLGTI DPYFVKLGDA MVTWIEAWDE LNPSTPAAAN GKAK

References

[1]	"Isolation of a maize 41 kDa Golgi associated protein." Katz A., Van Lent J.W.M., Kotlizky G., Yahalom A., Epel B.L. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Jubile.
[2]	"Beta-glucosylarginine: a new glucose-protein bond in a self-glucosylating protein from sweet corn." Singh D.G., Lomako J., Lomako W.M., Whelan W.J., Meyer H.E., Serwe M., Metzger J.W. FEBS Lett. 376:61-64(1995) [PubMed: 8521968] [Abstract] Cited for: PROTEIN SEQUENCE OF 31-57; 133-193; 199-208; 244-254; 289-307 AND 337-359, GLYCOSYLATION.
[3]	"The maize two dimensional gel protein database: towards an integrated genome analysis program." Touzet P., Riccardi F., Morin C., Damerval C., Huet J.-C., Pernollet J.-C., Zivy M., de Vienne D. Theor. Appl. Genet. 93:997-1005(1996) [Agricola: IND20551642] Cited for: PROTEIN SEQUENCE OF 61-75. Tissue: Coleoptile.
[4]	"UDP-glucose:protein transglucosylase in developing maize endosperm." Rothschild A., Tandecarz J.S. Plant Sci. 97:119-127(1994) Cited for: ENZYME ACTIVITY.
[5]	"A 41kDa protein isolated from maize mesocotyl cell walls immunolocalizes to plasmodesmata." Epel B.L., Van Lent J.W.M., Cohen L., Kotlizky G., Katz A., Yahalom A. Protoplasma 191:70-78(1996) Cited for: SUBCELLULAR LOCATION.
[6]	"Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor." Rothschild A., Wald F.A., Bocca S.N., Tandecarz J.S. Cell. Mol. Biol. 42:645-651(1996) [PubMed: 8832094] [Abstract] Cited for: INHIBITION BY IP.
[7]	"Identification of a UPTG inhibitor protein from maize endosperm: high homology with sucrose synthase protein." Wald F.A., Rothschild A., Moreno S., Tandecarz J.S. Cell. Mol. Biol. 44:397-406(1998) [PubMed: 9620435] [Abstract] Cited for: INHIBITION BY IP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U89897 mRNA. Translation: AAB49896.1.
PIR	T04331.
RefSeq	NP_001105598.1. NM_001112128.1.
UniGene	Zm.752.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT75. Glycosyltransferase Family 75.
Proteomic databases
PRIDE	P80607.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	542592.
KEGG	zma:542592.
Organism-specific databases
Gramene	P80607.
MaizeGDB	123920. 131466.
Phylogenomic databases
GeneTree	EPGT00050000010071.
Family and domain databases
InterPro	IPR004901. UPTG_synth. [Graphical view]
KO	K13379.
Pfam	PF03214. RGP. 1 hit. [Graphical view]
PIRSF	PIRSF016429. UPTG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPTG_MAIZE

Accession

Primary (citable) accession number: P80607
Secondary accession number(s): Q9SAQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 9, 2003
Last modified:	November 16, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program