Reviewed, UniProtKB/Swiss-Prot P80602 (BAS1_WHEAT)

Names and origin

Protein names

Recommended name:
    2-Cys peroxiredoxin BAS1, chloroplastic
    EC=1.11.1.15
Alternative name(s):
    Thiol-specific antioxidant protein

Gene names

Name:

TSA

Organism

Triticum aestivum (Wheat)

Taxonomic identifier

4565 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	210 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be an antioxidant enzyme particularly in the developing shoot and photosynthesizing leaf.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Subunit structure	Homodimer; disulfide-linked, upon oxidation By similarity.
Subcellular location	Plastid › chloroplast.
Post-translational modification	The Cys-64-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-64 (probably Cys-SOH) rapidly reacts with Cys-185-SH of the other subunit to form an intermolecular disulfide. This disulfide might subsequently be reduced by thioredoxin By similarity.
Sequence similarities	Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Redox-active center Transit peptide
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 10	›10	Chloroplast Ref.2
	Chain	11 – 210	200	2-Cys peroxiredoxin BAS1, chloroplastic		PRO_0000023787
Regions
	Domain	18 – 177	160	Thioredoxin
Sites
	Active site	64	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	64		Interchain (with C-185); in linked form By similarity
	Disulfide bond	185		Interchain (with C-64); in linked form By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P80602-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: E2D488179D6937E6	FASTA	210	23,327
	10 20 30 40 50 60 DARARSFVAR AAAEYDLPLV GNKAPDFAAE AVFDQEFINV KLSDYIGKKY VILFFYPLDF 70 80 90 100 110 120 TFVCPTEITA FSDRHEEFEK INTEILGVSV DSVFSHLAWV QTERKSGGLG DLKYPLVSDV 130 140 150 160 170 180 TKSISKSFGV LIPDQGIALR GLFIIDKEGV IQHSTINNLG IGRSVDETLR TLRALQYVKK 190 200 210 PDEVCPAGWK PGEKSMKPDP KGSKEYFAAI

References

[1]	Tsunoyama Y., Toyoshima Y. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Norin 61. Tissue: Seedling.
[2]	Tsunoyama Y., Takashi S., Toyoshima Y. Submitted (MAY-1996) to UniProtKB Cited for: PROTEIN SEQUENCE OF 11-35. Strain: cv. Norin 61. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB000405 mRNA. Translation: BAA19099.1.
PIR	T06318.
UniGene	Ta.28714
3D structure databases
HSSP	HSSP built from PDB template 1QMV based on UniProtKB P32119.
SMR	P80602. Positions 20-208.
ModBase	Search...
Protein family/group databases
PeroxiBase	4382. Ta2CysPrx.
Organism-specific databases
Gramene	P80602.
Enzyme and pathway databases
BRENDA	1.11.1.15. 253.
Family and domain databases
InterPro	IPR000866. Alkyl_hydroperoxide_Rdtase. IPR019479. Peroxiredoxin_C. IPR017936. Thioredoxin-like. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF10417. 1-cysPrx_C. 1 hit. PF00578. AhpC-TSA. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BAS1_WHEAT

Accession

Primary (citable) accession number: P80602

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)