Reviewed, UniProtKB/Swiss-Prot P80595 (APY_SOLTU)

Names and origin

Protein names

Recommended name:
    Apyrase
    EC=3.6.1.5
Alternative name(s):
    ATP-diphosphatase
    Adenosine diphosphatase
      Short name=ADPase
    ATP-diphosphohydrolase

Gene names

Name:

RROP1

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates.
Catalytic activity	ATP + 2 H2O = AMP + 2 phosphate.
Cofactor	Calcium.
Subcellular location	Membrane; Single-pass membrane protein Probable.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane
Ligand	ATP-binding Calcium Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 454	424	Apyrase		PRO_0000019904
Regions
	Transmembrane	426 – 446	21	Potential
Amino acid modifications
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P80595-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 9D9EFE431DA2F52F	FASTA	454	50,041
	10 20 30 40 50 60 MLNQNSHFIF IILAIFLVLP LSLLSKNVNA QIPLRRHLLS HESEHYAVIF DAGSTGSRVH 70 80 90 100 110 120 VFRFDEKLGL LPIGNNIEYF MATEPGLSSY AEDPKAAANS LEPLLDGAEG VVPQELQSET 130 140 150 160 170 180 PLELGATAGL RMLKGDAAEK ILQAVRNLVK NQSTFHSKDQ WVTILDGTQE GSYMWAAINY 190 200 210 220 230 240 LLGNLGKDYK STTATIDLGG GSVQMAYAIS NEQFAKAPQN EDGEPYVQQK HLMSKDYNLY 250 260 270 280 290 300 VHSYLNYGQL AGRAEIFKAS RNESNPCALE GCDGYYSYGG VDYKVKAPKK GSSWKRCRRL 310 320 330 340 350 360 TRHALKINAK CNIEECTFNG VWNGGGGDGQ KNIHASSFFY DIGAQVGIVD TKFPSALAKP 370 380 390 400 410 420 IQYLNAAKVA CQTNVADIKS IFPKTQDRNI PYLCMDLIYE YTLLVDGFGL NPHKEITVIH 430 440 450 DVQYKNYLVG AAWPLGCAID LVSSTTNKIR VASS

References

[1]

"Purification and cloning of a soluble ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum)." Handa M., Guidotti G. Biochem. Biophys. Res. Commun. 218:916-923(1996) [PubMed: 8579614] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 59-160; 236-253 AND 332-345. Tissue: Tuber.

[2]

"Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni. Immunological cross-reactivities with potato apyrase and Toxoplasma gondii nucleoside triphosphate hydrolase." Vasconcelos E.G., Ferreira S.T., de Carvalho T.M.U., de Souza W., Kettlun A.M., Mancilla M., Valenzuela M.A., Verjovski-Almeida S. J. Biol. Chem. 271:22139-22145(1996) [PubMed: 8703025] [Abstract] Cited for: PROTEIN SEQUENCE OF 42-54; 68-95 AND 236-253. Strain: cv. Desiree.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U58597 mRNA. Translation: AAB02720.1.
PIR	JC4616.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.6.1.5. 296.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. [Graphical view]
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APY_SOLTU

Accession

Primary (citable) accession number: P80595
Secondary accession number(s): Q43164

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	October 13, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)