P80595 (APY_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apyrase
EC=3.6.1.5

Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name=ADPase

Gene names

Name:

RROP1

Organism

Solanum tuberosum (Potato) [Reference proteome]

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates.
Catalytic activity	ATP + 2 H2O = AMP + 2 phosphate.
Cofactor	Calcium.
Subcellular location	Membrane; Single-pass type II membrane protein Probable.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	ATP-binding Calcium Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Apyrase		PRO_0000019904
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	29 – 454	426	Extracellular Potential
	Nucleotide binding	48 – 58	11	ATP-binding Probable
	Nucleotide binding	194 – 204	11	ATP-binding Probable
	Compositional bias	324 – 329	6	Poly-Gly
Sites
	Active site	170	1	Proton acceptor By similarity
Amino acid modifications
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P80595 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 9D9EFE431DA2F52F	FASTA	454	50,041
	10 20 30 40 50 60 MLNQNSHFIF IILAIFLVLP LSLLSKNVNA QIPLRRHLLS HESEHYAVIF DAGSTGSRVH 70 80 90 100 110 120 VFRFDEKLGL LPIGNNIEYF MATEPGLSSY AEDPKAAANS LEPLLDGAEG VVPQELQSET 130 140 150 160 170 180 PLELGATAGL RMLKGDAAEK ILQAVRNLVK NQSTFHSKDQ WVTILDGTQE GSYMWAAINY 190 200 210 220 230 240 LLGNLGKDYK STTATIDLGG GSVQMAYAIS NEQFAKAPQN EDGEPYVQQK HLMSKDYNLY 250 260 270 280 290 300 VHSYLNYGQL AGRAEIFKAS RNESNPCALE GCDGYYSYGG VDYKVKAPKK GSSWKRCRRL 310 320 330 340 350 360 TRHALKINAK CNIEECTFNG VWNGGGGDGQ KNIHASSFFY DIGAQVGIVD TKFPSALAKP 370 380 390 400 410 420 IQYLNAAKVA CQTNVADIKS IFPKTQDRNI PYLCMDLIYE YTLLVDGFGL NPHKEITVIH 430 440 450 DVQYKNYLVG AAWPLGCAID LVSSTTNKIR VASS

References

[1]

"Purification and cloning of a soluble ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum)." Handa M., Guidotti G. Biochem. Biophys. Res. Commun. 218:916-923(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 59-160; 236-253 AND 332-345. Tissue: Tuber.

[2]

"Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni. Immunological cross-reactivities with potato apyrase and Toxoplasma gondii nucleoside triphosphate hydrolase." Vasconcelos E.G., Ferreira S.T., de Carvalho T.M.U., de Souza W., Kettlun A.M., Mancilla M., Valenzuela M.A., Verjovski-Almeida S. J. Biol. Chem. 271:22139-22145(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 42-54; 68-95 AND 236-253. Strain: cv. Desiree.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U58597 mRNA. Translation: AAB02720.1.
PIR	JC4616.
3D structure databases
ProteinModelPortal	P80595.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16862.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. [Graphical view]
PANTHER	PTHR11782. PTHR11782. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APY_SOLTU

Accession

Primary (citable) accession number: P80595
Secondary accession number(s): Q43164

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program