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P80595 (APY_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apyrase

EC=3.6.1.5
Alternative name(s):
ATP-diphosphatase
ATP-diphosphohydrolase
Adenosine diphosphatase
Short name=ADPase
Gene names
Name:RROP1
OrganismSolanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier4113 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum

Protein attributes

Sequence length454 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the hydrolysis of phosphoanhydride bonds of nucleoside tri- and di-phosphates.

Catalytic activity

A nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate.

Cofactor

Calcium.

Subcellular location

Membrane; Single-pass type II membrane protein Probable.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
   Cellular componentMembrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandATP-binding
Calcium
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

hydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 454454Apyrase
PRO_0000019904

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2821Helical; Signal-anchor for type II membrane protein; Potential
Topological domain29 – 454426Extracellular Potential
Nucleotide binding48 – 5811ATP-binding Probable
Nucleotide binding194 – 20411ATP-binding Probable
Compositional bias324 – 3296Poly-Gly

Sites

Active site1701Proton acceptor By similarity

Amino acid modifications

Glycosylation1511N-linked (GlcNAc...) Potential
Glycosylation2621N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P80595 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 9D9EFE431DA2F52F

FASTA45450,041
        10         20         30         40         50         60 
MLNQNSHFIF IILAIFLVLP LSLLSKNVNA QIPLRRHLLS HESEHYAVIF DAGSTGSRVH 

        70         80         90        100        110        120 
VFRFDEKLGL LPIGNNIEYF MATEPGLSSY AEDPKAAANS LEPLLDGAEG VVPQELQSET 

       130        140        150        160        170        180 
PLELGATAGL RMLKGDAAEK ILQAVRNLVK NQSTFHSKDQ WVTILDGTQE GSYMWAAINY 

       190        200        210        220        230        240 
LLGNLGKDYK STTATIDLGG GSVQMAYAIS NEQFAKAPQN EDGEPYVQQK HLMSKDYNLY 

       250        260        270        280        290        300 
VHSYLNYGQL AGRAEIFKAS RNESNPCALE GCDGYYSYGG VDYKVKAPKK GSSWKRCRRL 

       310        320        330        340        350        360 
TRHALKINAK CNIEECTFNG VWNGGGGDGQ KNIHASSFFY DIGAQVGIVD TKFPSALAKP 

       370        380        390        400        410        420 
IQYLNAAKVA CQTNVADIKS IFPKTQDRNI PYLCMDLIYE YTLLVDGFGL NPHKEITVIH 

       430        440        450 
DVQYKNYLVG AAWPLGCAID LVSSTTNKIR VASS 

« Hide

References

[1]"Purification and cloning of a soluble ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum)."
Handa M., Guidotti G.
Biochem. Biophys. Res. Commun. 218:916-923(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 59-160; 236-253 AND 332-345.
Tissue: Tuber.
[2]"Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni. Immunological cross-reactivities with potato apyrase and Toxoplasma gondii nucleoside triphosphate hydrolase."
Vasconcelos E.G., Ferreira S.T., de Carvalho T.M.U., de Souza W., Kettlun A.M., Mancilla M., Valenzuela M.A., Verjovski-Almeida S.
J. Biol. Chem. 271:22139-22145(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 42-54; 68-95 AND 236-253.
Strain: cv. Desiree.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U58597 mRNA. Translation: AAB02720.1.
PIRJC4616.
UniGeneStu.216.

3D structure databases

ProteinModelPortalP80595.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16862.

Family and domain databases

InterProIPR000407. GDA1_CD39_NTPase.
[Graphical view]
PANTHERPTHR11782. PTHR11782. 1 hit.
PfamPF01150. GDA1_CD39. 1 hit.
[Graphical view]
PROSITEPS01238. GDA1_CD39_NTPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAPY_SOLTU
AccessionPrimary (citable) accession number: P80595
Secondary accession number(s): Q43164
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families