Reviewed, UniProtKB/Swiss-Prot P80576 (AROF_NEUCR)

Names and origin

Protein names

Recommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase
    EC=2.5.1.54
Alternative name(s):
    Phospho-2-keto-3-deoxyheptonate aldolase
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
    DAHP synthetase

Gene names

Name:	aro-8
Synonyms:	aroh
ORF Names:	NCU02785

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Subunit structure	Homodimer By similarity.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the class-II DAHP synthetase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Phospho-2-dehydro-3-deoxyheptonate aldolase		PRO_0000140853
Experimental info
	Sequence conflict	251	1	R → T AA sequence Ref.2
	Sequence conflict	255	1	H → A AA sequence Ref.2
	Sequence conflict	258	1	V → Q AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P80576-1 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: F909473F599FD9BF	FASTA	481	54,067
	10 20 30 40 50 60 MSQQTTPNAP GWAPDSWRSK PIKQCPEYPD KAALEKATNE LKTLPPIVLP NEIIRLREHL 70 80 90 100 110 120 RDVAQGKAFL LQGGDCAELF SYCQQDVIES KIKLLLQMSL VLLWGADKPV VRIGRMAGQY 130 140 150 160 170 180 AKPRSSPVET INGKEVPSFR GDILNGFHPD ERELDPNRLV RAYQYSSATL NYIRGAIGSG 190 200 210 220 230 240 IADLHGPLDW GLGHVRDPAL KSKYQETVDR IQEMLRFMHT IGADQNEKLS TVELFTSHEG 250 260 270 280 290 300 LLLEYEEPLT RLLNHPSVRS YPPDSTTPPK KEYYNTSAHF LWIGDRTRQI DHAHVEYFRG 310 320 330 340 350 360 IANPIGVKIG PSTPTSDLLP MLRTLNPNRE PGKVTLITRY GADKVASLLP AHIRTVESSE 370 380 390 400 410 420 YARTVVWQCD PMHGNTQSVS GGIKTRKFSD IFSELQQTLR IHKEEKSYLG GMHLELTGDA 430 440 450 460 470 480 VTECLGGGAG LDEDDLSTNY TSFCDPRLNE KQALELAFLV ADHYRQERKE KEAERRKSSV V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"Evidence for a novel class of microbial 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase in Streptomyces coelicolor A3(2), Streptomyces rimosus and Neurospora crassa." Walker G.E., Dunbar B., Hunter I.S., Nimmo H.G., Coggins J.R. Microbiology 142:1973-1982(1996) [PubMed: 8760910] [Abstract] Cited for: PROTEIN SEQUENCE OF 43-72 AND 204-258. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AABX02000007 Genomic DNA. Translation: EAA34705.1.
RefSeq	XP_963941.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P80576.
Genome annotation databases
GeneID	3880090.
KEGG	ncr:NCU02785.
NMPDR	fig|5141.1.peg.7906.
Enzyme and pathway databases
BRENDA	2.5.1.54. 266.
Family and domain databases
InterPro	IPR002480. DAHP_synth_2. [Graphical view]
PANTHER	PTHR21337. DAHP_synth_2. 1 hit.
Pfam	PF01474. DAHP_synth_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROF_NEUCR

Accession

Primary (citable) accession number: P80576
Secondary accession number(s): Q7SD77

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 31, 2004
Last modified:	September 22, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)