Reviewed, UniProtKB/Swiss-Prot P80574 (AROF_STRCO)

Names and origin

Protein names

Recommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase
    EC=2.5.1.54
Alternative name(s):
    Phospho-2-keto-3-deoxyheptonate aldolase
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
    DAHP synthetase

Gene names

Name:	aroH
Ordered Locus Names:	SCO2115
ORF Names:	SC6E10.09c

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the class-II DAHP synthetase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 450	449	Phospho-2-dehydro-3-deoxyheptonate aldolase		PRO_0000140854
Experimental info
	Sequence conflict	15 – 22	8	WRDLPAAQ → DDPLQAPS AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P80574-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 074668C290250874	FASTA	450	49,870
	10 20 30 40 50 60 MTVNAKTSPS AGNTWRDLPA AQQPEYPDTE ALRAVIADLE SYPPLVFAGE CDQLRARMAA 70 80 90 100 110 120 VAKGEAFLLQ GGDCAEAFDA VSADHIRNKL KTLLQMGAVL TYAASVPVVK VGRIAGQYSK 130 140 150 160 170 180 PRSKPTETRD GVTLPTYRGD SVNGFDFTEA ARIPDPERLK RMYHASASTL NLVRAFTTGG 190 200 210 220 230 240 YADLRQVHAW NQDFVKSSPS GQRYEQLARE IDNALNFMRA CGTDPAEFQT VEFFSSHEAL 250 260 270 280 290 300 LLDYESALTR VDSRTGQLYD VSGHMVWIGE RTRQLDHAHI EFASRIRNPI GIKLGPSTTA 310 320 330 340 350 360 EEALQYIERL DPEREPGRLT FIVRMGADKI RDKLPELVEK VTASGATVAW ITDPMHGNTY 370 380 390 400 410 420 EAASGHKTRR FDDVLDEVKG FFEVHKSLGT HPGGIHVELT GDDVTECVGG GDEIFVDDLH 430 440 450 QRYETACDPR LNRSQSLDLA FLVAEMYRDQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[2]	"Evidence for a novel class of microbial 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase in Streptomyces coelicolor A3(2), Streptomyces rimosus and Neurospora crassa." Walker G.E., Dunbar B., Hunter I.S., Nimmo H.G., Coggins J.R. Microbiology 142:1973-1982(1996) [PubMed: 8760910] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-22, CHARACTERIZATION. Strain: A3(2).

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL939111 Genomic DNA. Translation: CAB51963.1.
PIR	T35496.
RefSeq	NP_626372.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1097549.
GenomeReviews	Gene locus SCO2115 in contig AL645882_GR.
KEGG	sco:SCO2115.
NMPDR	fig|100226.1.peg.2081.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P80574.
OMA	YDTSAHF.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO2115-MON.
BRENDA	2.5.1.54. 1084.
Family and domain databases
InterPro	IPR002480. DAHP_synth_2. [Graphical view]
PANTHER	PTHR21337. DAHP_synth_2. 1 hit.
Pfam	PF01474. DAHP_synth_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01358. DAHP_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROF_STRCO

Accession

Primary (citable) accession number: P80574
Secondary accession number(s): Q9S2M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)