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P80563 (PGTL_PELAC) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyrogallol hydroxytransferase large subunit

EC=1.97.1.2
Alternative name(s):
Transhydroxylase subunit alpha
Gene names
Name:athL
OrganismPelobacter acidigallici
Taxonomic identifier35816 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesPelobacteraceaePelobacter

Protein attributes

Sequence length875 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Isomerisation of pyrogallol to phloroglucin.

Catalytic activity

1,2,3,5-tetrahydroxybenzene + 1,2,3-trihydroxybenzene = 1,3,5-trihydroxybenzene + 1,2,3,5-tetrahydroxybenzene.

Cofactor

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit. Ref.4

Subunit structure

Heterodimer of a large and a small subunit. Ref.4

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 875874Pyrogallol hydroxytransferase large subunit
PRO_0000063242

Sites

Metal binding1751Molybdenum

Secondary structure

.......................................................................................................................................................................................... 875
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P80563 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: B5D7DE5FAEF53A61

FASTA87599,261
        10         20         30         40         50         60 
MGEVVRLTNS STGGPVFVYV KDGKIIRMTP MDFDDAVDAP SWKIEARGKT FTPPRKTSIA 

        70         80         90        100        110        120 
PYTAGFKSMI YSDLRIPYPM KRKSFDPNGE RNPQLRGAGL SKQDPWSDYE RISWDEATDI 

       130        140        150        160        170        180 
VVAEINRIKH AYGPSAILST PSSHHMWGNV GYRHSTYFRF MNMMGFTYAD HNPDSWEGWH 

       190        200        210        220        230        240 
WGGMHMWGFS WRLGNPEQYD LLEDGLKHAE MIVFWSSDPE TNSGIYAGFE SNIRRQWLKD 

       250        260        270        280        290        300 
LGVDFVFIDP HMNHTARLVA DKWFSPKIGT DHALSFAIAY TWLKEDSYDK EYVAANAHGF 

       310        320        330        340        350        360 
EEWADYVLGK TDGTPKTCEW AEEESGVPAC EIRALARQWA KKNTYLAAGG LGGWGGACRA 

       370        380        390        400        410        420 
SHGIEWARGM IALATMQGMG KPGSNMWSTT QGVPLDYEFY FPGYAEGGIS GDCENSAAGF 

       430        440        450        460        470        480 
KFAWRMFDGK TTFPSPSNLN TSAGQHIPRL KIPECIMGGK FQWSGKGFAG GDISHQLHQY 

       490        500        510        520        530        540 
EYPAPGYSKI KMFWKYGGPH LGTMTATNRY AKMYTHDSLE FVVSQSIWFE GEVPFADIIL 

       550        560        570        580        590        600 
PACTNFERWD ISEFANCSGY IPDNYQLCNH RVISLQAKCI EPVGESMSDY EIYRLFAKKL 

       610        620        630        640        650        660 
NIEEMFSEGK DELAWCEQYF NATDMPKYMT WDEFFKKGYF VVPDNPNRKK TVALRWFAEG 

       670        680        690        700        710        720 
REKDTPDWGP RLNNQVCRKG LQTTTGKVEF IATSLKNFEE QGYIDEHRPS MHTYVPAWES 

       730        740        750        760        770        780 
QKHSPLAVKY PLGMLSPHPR FSMHTMGDGK NSYMNYIKDH RVEVDGYKYW IMRVNSIDAE 

       790        800        810        820        830        840 
ARGIKNGDLI RAYNDRGSVI LAAQVTECLQ PGTVHSYESC AVYDPLGTAG KSADRGGCIN 

       850        860        870 
ILTPDRYISK YACGMANNTA LVEIEKWDGD KYEIY 

« Hide

References

[1]Retey J.
Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"One molecule of molybdopterin guanine dinucleotide is associated with each subunit of the heterodimeric Mo-Fe-S protein transhydroxylase of Pelobacter acidigallici as determined by SDS/PAGE and mass spectrometry."
Reichenbecher W., Ruediger A., Kroneck P.M.H., Schink B.
Eur. J. Biochem. 237:406-413(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-17.
Strain: DSM 2377 / Braunschweig.
[3]"Towards the reaction mechanism of pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici."
Reichenbecher W., Schink B.
Biochim. Biophys. Acta 1430:245-253(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Crystal structure of pyrogallol-phloroglucinol transhydroxylase, an Mo enzyme capable of intermolecular hydroxyl transfer between phenols."
Messerschmidt A., Niessen H., Abt D., Einsle O., Schink B., Kroneck P.M.
Proc. Natl. Acad. Sci. U.S.A. 101:11571-11576(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-874 IN COMPLEXES WITH MO-BIS-MGD; SUBSTRATE OR INHIBITOR AND THE SMALL SUBUNIT, COFACTOR, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ243850 Genomic DNA. Translation: CAB50913.1.
PIRS65430.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1TI2X-ray2.35A/C/E/G/I/K2-874[»]
1TI4X-ray2.20A/C/E/G/I/K2-874[»]
1TI6X-ray2.00A/C/E/G/I/K2-874[»]
1VLDX-ray2.35M/O/Q/S/U/W2-874[»]
1VLEX-ray2.20M/O/Q/S/U/W2-874[»]
1VLFX-ray2.00M/O/Q/S/U/W2-874[»]
ProteinModelPortalP80563.
SMRP80563. Positions 1-875.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB5.A.3.7.1. the prokaryotic molybdopterin-containing oxidoreductase (pmo) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
[Graphical view]
PfamPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP80563.

Entry information

Entry namePGTL_PELAC
AccessionPrimary (citable) accession number: P80563
Secondary accession number(s): Q9S354
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: October 16, 2013
This is version 67 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references