P80562 (IPYR_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	all3570

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes PPi generated in anabolic reactions. HAMAP MF_00209
Catalytic activity	Diphosphate + H2O = 2 phosphate. Ref.1
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity. In the presence of zinc, manganese, copper, iron or cobalt ions, activity is very low. In the absence of metal ions, there is no activity. Ref.1
Subunit structure	Homohexamer. Ref.1
Subcellular location	Cytoplasm HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.
Biophysicochemical properties	Kinetic parameters: KM=3 µM for PPi Ref.1

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Formylation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137474
Sites
	Metal binding	64	1	Magnesium 1 By similarity
	Metal binding	69	1	Magnesium 1 By similarity
	Metal binding	69	1	Magnesium 2 By similarity
	Metal binding	101	1	Magnesium 1 By similarity
Amino acid modifications
	Modified residue	1	1	N-formylmethionine HAMAP MF_00209
Experimental info
	Sequence conflict	117	1	V → R in CAC81009. Ref.1
	Sequence conflict	148 – 149	2	IL → SV in CAC81009. Ref.1
	Sequence conflict	163 – 165	3	QSI → HPV in CAC81009. Ref.1
	Sequence conflict	169	1	K → T in CAC81009. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P80562 [UniParc]. Last modified February 11, 2002. Version 1. Checksum: 17A027E16C2289D3	FASTA	169	18,961
	10 20 30 40 50 60 MDLSRIPAQP KPGVINILIE IAGGSQNKYE FDKDLEAFAL DRVLYSSVKY PYDYGFVPNT 70 80 90 100 110 120 LADDGDPLDG MVIIDEPTFP GCVIAARPIG FLEMIDGGDR DEKILAVPDK DPRYAHVKSL 130 140 150 160 NDVAPHRLDE IAEFFRSYKN LEKKVTQILG WQDVDQVKAL VDQSIKAYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria." Gomez-Garcia M.R., Losada M., Serrano A. FEBS J. 274:3948-3959(2007) [PubMed: 17635582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, FORMYLATION AT MET-1.
[2]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ252206 Genomic DNA. Translation: CAC81009.2. BA000019 Genomic DNA. Translation: BAB75269.1.
PIR	AC2252.
RefSeq	NP_487610.1. NC_003272.1.
3D structure databases
ProteinModelPortal	P80562.
ModBase	Search...
Protein-protein interaction databases
STRING	P80562.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1107168.
GenomeReviews	Gene locus all3570 in contig BA000019_GR.
KEGG	ana:all3570.
NMPDR	fig|103690.1.peg.3877.
PATRIC	22777570. VBINosSp37423_4240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0221.
HOGENOM	HBG529150.
OMA	PNDFNVI.
PhylomeDB	P80562.
ProtClustDB	CLSK895996.
Enzyme and pathway databases
BioCyc	NSP103690:ALL3570-MONOMER.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_NOSS1

Accession

Primary (citable) accession number: P80562
Secondary accession number(s): Q8YR78

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	February 11, 2002
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program