P80560 (PTPR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Receptor-type tyrosine-protein phosphatase N2
Short name=R-PTP-N2
EC=3.1.3.48

Alternative name(s):
PTP IA-2beta
Protein tyrosine phosphatase-NP
Short name=PTP-NP

Gene names

Name:

Ptprn2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1001 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in development of nervous system and pancreatic endocrine cells.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subcellular location	Cytoplasmic vesicle › secretory vesicle membrane; Single-pass type I membrane protein By similarity.
Tissue specificity	Pancreas and brain.
Developmental stage	Expressed in early stages of pancreatic development. First expressed in day 8.5 embryos (E8.5) in the dorsal part of the midgut endoderm and by E9.5, in the pancreatic rudiment specifically in early endocrine progenitor cells. At later stages expressed in insulin- or glucagon-producing cells. During neural development, the type 2 PTP-NP is expressed in early stages of neurogenesis, and the type 1 weakly in the later stages.
Post-translational modification	Appears to undergo multiple proteolytic cleavage at consecutive basic residues By similarity.
Involvement in disease	Autoantigen in insulin-dependent diabetes mellitus (IDDM).
Disruption phenotype	Mild glucose intolerance and impaired glucose-stimulated insulin secretion. Ref.3
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Disease	Diabetes mellitus
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Hydrolase Protein phosphatase Receptor
PTM	Acetylation Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of GTPase activity Inferred from electronic annotation. Source: Compara peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW secretory granule Inferred from direct assay PubMed 15788565. Source: MGI terminal bouton Inferred from electronic annotation. Source: Compara transport vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P80560-1)

Also known as: Type 1-PTP-NP;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P80560-2)

Also known as: Type 2-PTP-NP;

The sequence of this isoform is not available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 1001	974	Receptor-type tyrosine-protein phosphatase N2		PRO_0000025456
Regions
	Topological domain	28 – 600	573	Extracellular Potential
	Transmembrane	601 – 621	21	Helical; Potential
	Topological domain	622 – 1001	380	Cytoplasmic Potential
	Domain	731 – 991	261	Tyrosine-protein phosphatase
	Region	931 – 937	7	Substrate binding By similarity
Sites
	Active site	931	1	Phosphocysteine intermediate Potential
	Binding site	899	1	Substrate By similarity
	Binding site	976	1	Substrate By similarity
	Site	413 – 414	2	Cleavage By similarity
Amino acid modifications
	Modified residue	678	1	Phosphoserine Ref.4
	Modified residue	956	1	N6-acetyllysine By similarity
	Glycosylation	550	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	121	1	A → T in AAB06945. Ref.1
	Sequence conflict	266	1	A → S in AAB06945. Ref.1
	Sequence conflict	369	1	E → D in AAB06945. Ref.1
	Sequence conflict	370 – 371	2	Missing in AAB39996. Ref.2
	Sequence conflict	395	1	P → H in AAB06945. Ref.1
	Sequence conflict	411	1	I → M in AAB39996. Ref.2
	Sequence conflict	414	1	S → L in AAB39996. Ref.2
	Sequence conflict	586	1	L → H in AAB39996. Ref.2
	Sequence conflict	662	1	I → V in AAB06945. Ref.1
	Sequence conflict	662	1	I → V in AAB39996. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Type 1-PTP-NP) [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 42B590A13CA89CB8	FASTA	1,001	111,497
	10 20 30 40 50 60 MGPPLPLLLL LLLPPPLPRA LPAPASARGR QLPGRLGCLF EDGLCGSLET CVNDGVFGRC 70 80 90 100 110 120 QKVPVMDTYR YEVPPGALLH LKVTLQKLSR TGFTWQDDYT QRVIAQELAN LPKAYLWHGE 130 140 150 160 170 180 ASGPARSLQQ NADNEKWFSL EREVALAKTL RRYLPYLELL SQTPTANAHS RIDHETRPAK 190 200 210 220 230 240 GEDSSPENIL TYVAHTSALT YPPATRAKYP DNLLRPFSRL QPDELSPKVD GDIDKQKLIA 250 260 270 280 290 300 ALGAYTAQRL PGENDPEPRY LVHGSARAPR PFSATALSQR WPPPPGDAKD SPSMDDDTLL 310 320 330 340 350 360 QSLLKDLQQN SEVDRLGPLK EEKADSVAGA IQSDPAEGSQ ESHGRGAEGQ PREQTDAPET 370 380 390 400 410 420 MLQDHRLSEV DDPVYKEVNR LSFQLGDLLK DYGSPLLPEG PLLEKSSREE IKKSEQPEEV 430 440 450 460 470 480 LSSEEETAGV EHVRSRTYSK DLFERKPNSE PQPRRLEDQF QNRAPELWED EESLKLAAQG 490 500 510 520 530 540 PPSGGLQLEV QPSEEQQGYI LTGNNPLSPE KGKQLMDQVA HILRVPSSFF ADIKVLGPAV 550 560 570 580 590 600 TFKVSANIQN MTTADVIKAA ADNKDQLEKA TGLTILQSGI RPKGKLKLLP HQEEQEDSTK 610 620 630 640 650 660 FILLTFLSIA CILGVLLASS LAYCLRHNSH YKLKDKLSGL GADPSADATE AYQELCRQRM 670 680 690 700 710 720 AIRPQDRSEG PHTSRINSVS SQFSDGPMPS PSARSSTSSW SEEPVQSNMD ISTGHMILAY 730 740 750 760 770 780 MEDHLKNKNR LEKEWEALCA YQAEPNSSLV AQREENAPKN RSLAVLTYDH SRILLKSQNS 790 800 810 820 830 840 HGSSDYINAS PIMDHDPRNP AYIATQGPLP ATVADFWQMV WESGCAVIVM LTPLSENGVR 850 860 870 880 890 900 QCHHYWPDEG SNLYHVYEVN LVSEHIWCQD FLVRSFYLKN LQTNETRTVT QFHFLSWYDQ 910 920 930 940 950 960 GVPSSTRSLL DFRRKVNKCY RGRSCPIIVH CSDGAGRSGT YVLIDMVLNK MAKGAKEIDI 970 980 990 1000 AATLEHLRDQ RPGMVQTKEQ FEFALTAVAE EVNAILKALP Q
	Isoform 2 (Type 2-PTP-NP) (Sequence not available).		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PTP-NP, a new member of the receptor protein tyrosine phosphatase family, implicated in development of nervous system and pancreatic endocrine cells." Chiang M.-K., Flanagan J.G. Development 122:2239-2250(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ICR. Tissue: Brain.
[2]	"Identification of a second transmembrane protein tyrosine phosphatase, IA-2beta, as an autoantigen in insulin-dependent diabetes mellitus: precursor of the 37-kDa tryptic fragment." Lu J., Li Q., Xie H., Chen Z.-J., Borovitskaya A.E., Maclaren N.K., Notkins A.L., Lan M.S. Proc. Natl. Acad. Sci. U.S.A. 93:2307-2311(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 282-1001. Tissue: Neonatal brain.
[3]	"Targeted disruption of the IA-2beta gene causes glucose intolerance and impairs insulin secretion but does not prevent the development of diabetes in NOD mice." Kubosaki A., Gross S., Miura J., Saeki K., Zhu M., Nakamura S., Hendriks W., Notkins A.L. Diabetes 53:1684-1691(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, DISRUPTION PHENOTYPE. Strain: C57BL/6.
[4]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-678, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U57345 mRNA. Translation: AAB06945.1. U82439 mRNA. Translation: AAB39996.1. BN000315 , BN000293, BN000294, BN000295, BN000296, BN000297, BN000298, BN000299, BN000300, BN000301, BN000302, BN000303, BN000304, BN000305, BN000306, BN000307, BN000308, BN000309, BN000310, BN000311, BN000312, BN000313, BN000314 Genomic DNA. Translation: CAG23871.1.
IPI	IPI00118191.
RefSeq	NP_035345.2. NM_011215.2.
UniGene	Mm.206054.
3D structure databases
ProteinModelPortal	P80560.
SMR	P80560. Positions 497-583, 710-996.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000064046.
PTM databases
PhosphoSite	P80560.
Proteomic databases
PaxDb	P80560.
PRIDE	P80560.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000070733; ENSMUSP00000064046; ENSMUSG00000056553.
GeneID	19276.
KEGG	mmu:19276.
Organism-specific databases
CTD	5799.
MGI	MGI:107418. Ptprn2.
Phylogenomic databases
eggNOG	COG5599.
GeneTree	ENSGT00690000102184.
HOGENOM	HOG000243992.
HOVERGEN	HBG105788.
InParanoid	Q1RLJ1.
KO	K07817.
OMA	YVAHTSA.
OrthoDB	EOG4J6RQ4.
Gene expression databases
ArrayExpress	P80560.
Bgee	P80560.
Genevestigator	P80560.
GermOnline	ENSMUSG00000056553. Mus musculus.
Family and domain databases
InterPro	IPR021613. Receptor_IA-2. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. IPR000242. Tyr_Pase_rcpt/non-rcpt. [Graphical view]
Pfam	PF11548. Receptor_IA-2. 1 hit. PF00102. Y_phosphatase. 1 hit. [Graphical view]
PRINTS	PR00700. PRTYPHPHTASE.
SMART	SM00194. PTPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50055. TYR_PHOSPHATASE_PTP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	296180.
SOURCE	Search...

Entry information

Entry name

PTPR2_MOUSE

Accession

Primary (citable) accession number: P80560
Secondary accession number(s): O09134, P70328, Q1RLJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program