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P80560

- PTPR2_MOUSE

UniProt

P80560 - PTPR2_MOUSE

Protein

Receptor-type tyrosine-protein phosphatase N2

Gene

Ptprn2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Implicated in development of nervous system and pancreatic endocrine cells.

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei413 – 4142CleavageBy similarity
    Binding sitei899 – 8991SubstrateBy similarity
    Active sitei931 – 9311Phosphocysteine intermediatePROSITE-ProRule annotation
    Binding sitei976 – 9761SubstrateBy similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. negative regulation of GTPase activity Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase, Receptor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Receptor-type tyrosine-protein phosphatase N2 (EC:3.1.3.48)
    Short name:
    R-PTP-N2
    Alternative name(s):
    PTP IA-2beta
    Protein tyrosine phosphatase-NP
    Short name:
    PTP-NP
    Gene namesi
    Name:Ptprn2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:107418. Ptprn2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW
    3. receptor complex Source: MGI
    4. secretory granule Source: MGI
    5. terminal bouton Source: Ensembl
    6. transport vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Autoantigen in insulin-dependent diabetes mellitus (IDDM).

    Disruption phenotypei

    Mild glucose intolerance and impaired glucose-stimulated insulin secretion.1 Publication

    Keywords - Diseasei

    Diabetes mellitus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 1001974Receptor-type tyrosine-protein phosphatase N2PRO_0000025456Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi550 – 5501N-linked (GlcNAc...)Sequence Analysis
    Modified residuei956 – 9561N6-acetyllysineBy similarity

    Post-translational modificationi

    Appears to undergo multiple proteolytic cleavage at consecutive basic residues.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein

    Proteomic databases

    MaxQBiP80560.
    PaxDbiP80560.
    PRIDEiP80560.

    PTM databases

    PhosphoSiteiP80560.

    Expressioni

    Tissue specificityi

    Pancreas and brain.

    Developmental stagei

    Expressed in early stages of pancreatic development. First expressed in day 8.5 embryos (E8.5) in the dorsal part of the midgut endoderm and by E9.5, in the pancreatic rudiment specifically in early endocrine progenitor cells. At later stages expressed in insulin- or glucagon-producing cells. During neural development, the type 2 PTP-NP is expressed in early stages of neurogenesis, and the type 1 weakly in the later stages.

    Gene expression databases

    BgeeiP80560.
    GenevestigatoriP80560.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PtprnQ606734EBI-8538944,EBI-8328895

    Protein-protein interaction databases

    IntActiP80560. 2 interactions.
    MINTiMINT-8310377.
    STRINGi10090.ENSMUSP00000064046.

    Structurei

    3D structure databases

    ProteinModelPortaliP80560.
    SMRiP80560. Positions 497-583, 710-996.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 600573ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini622 – 1001380CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei601 – 62121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini731 – 991261Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni931 – 9377Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5599.
    GeneTreeiENSGT00750000117312.
    HOGENOMiHOG000243992.
    HOVERGENiHBG105788.
    InParanoidiQ1RLJ1.
    KOiK07817.
    OMAiYRYEVSP.
    OrthoDBiEOG7K9K30.
    TreeFamiTF351976.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR021613. Receptor_IA-2_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view]
    PfamiPF11548. Receptor_IA-2. 1 hit.
    PF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: P80560-1) [UniParc]FASTAAdd to Basket

    Also known as: Type 1-PTP-NP

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGPPLPLLLL LLLPPPLPRA LPAPASARGR QLPGRLGCLF EDGLCGSLET     50
    CVNDGVFGRC QKVPVMDTYR YEVPPGALLH LKVTLQKLSR TGFTWQDDYT 100
    QRVIAQELAN LPKAYLWHGE ASGPARSLQQ NADNEKWFSL EREVALAKTL 150
    RRYLPYLELL SQTPTANAHS RIDHETRPAK GEDSSPENIL TYVAHTSALT 200
    YPPATRAKYP DNLLRPFSRL QPDELSPKVD GDIDKQKLIA ALGAYTAQRL 250
    PGENDPEPRY LVHGSARAPR PFSATALSQR WPPPPGDAKD SPSMDDDTLL 300
    QSLLKDLQQN SEVDRLGPLK EEKADSVAGA IQSDPAEGSQ ESHGRGAEGQ 350
    PREQTDAPET MLQDHRLSEV DDPVYKEVNR LSFQLGDLLK DYGSPLLPEG 400
    PLLEKSSREE IKKSEQPEEV LSSEEETAGV EHVRSRTYSK DLFERKPNSE 450
    PQPRRLEDQF QNRAPELWED EESLKLAAQG PPSGGLQLEV QPSEEQQGYI 500
    LTGNNPLSPE KGKQLMDQVA HILRVPSSFF ADIKVLGPAV TFKVSANIQN 550
    MTTADVIKAA ADNKDQLEKA TGLTILQSGI RPKGKLKLLP HQEEQEDSTK 600
    FILLTFLSIA CILGVLLASS LAYCLRHNSH YKLKDKLSGL GADPSADATE 650
    AYQELCRQRM AIRPQDRSEG PHTSRINSVS SQFSDGPMPS PSARSSTSSW 700
    SEEPVQSNMD ISTGHMILAY MEDHLKNKNR LEKEWEALCA YQAEPNSSLV 750
    AQREENAPKN RSLAVLTYDH SRILLKSQNS HGSSDYINAS PIMDHDPRNP 800
    AYIATQGPLP ATVADFWQMV WESGCAVIVM LTPLSENGVR QCHHYWPDEG 850
    SNLYHVYEVN LVSEHIWCQD FLVRSFYLKN LQTNETRTVT QFHFLSWYDQ 900
    GVPSSTRSLL DFRRKVNKCY RGRSCPIIVH CSDGAGRSGT YVLIDMVLNK 950
    MAKGAKEIDI AATLEHLRDQ RPGMVQTKEQ FEFALTAVAE EVNAILKALP 1000
    Q 1001
    Length:1,001
    Mass (Da):111,497
    Last modified:July 27, 2011 - v2
    Checksum:i42B590A13CA89CB8
    GO
    Isoform 2 (identifier: P80560-2)

    Also known as: Type 2-PTP-NP

    Sequence is not available
    Length:
    Mass (Da):

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti121 – 1211A → T in AAB06945. (PubMed:8681804)Curated
    Sequence conflicti266 – 2661A → S in AAB06945. (PubMed:8681804)Curated
    Sequence conflicti369 – 3691E → D in AAB06945. (PubMed:8681804)Curated
    Sequence conflicti370 – 3712Missing in AAB39996. (PubMed:8637868)Curated
    Sequence conflicti395 – 3951P → H in AAB06945. (PubMed:8681804)Curated
    Sequence conflicti411 – 4111I → M in AAB39996. (PubMed:8637868)Curated
    Sequence conflicti414 – 4141S → L in AAB39996. (PubMed:8637868)Curated
    Sequence conflicti586 – 5861L → H in AAB39996. (PubMed:8637868)Curated
    Sequence conflicti662 – 6621I → V in AAB06945. (PubMed:8681804)Curated
    Sequence conflicti662 – 6621I → V in AAB39996. (PubMed:8637868)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57345 mRNA. Translation: AAB06945.1.
    U82439 mRNA. Translation: AAB39996.1.
    BN000315
    , BN000293, BN000294, BN000295, BN000296, BN000297, BN000298, BN000299, BN000300, BN000301, BN000302, BN000303, BN000304, BN000305, BN000306, BN000307, BN000308, BN000309, BN000310, BN000311, BN000312, BN000313, BN000314 Genomic DNA. Translation: CAG23871.1.
    CCDSiCCDS36576.1. [P80560-1]
    RefSeqiNP_035345.2. NM_011215.2. [P80560-1]
    UniGeneiMm.206054.

    Genome annotation databases

    EnsembliENSMUST00000070733; ENSMUSP00000064046; ENSMUSG00000056553. [P80560-1]
    GeneIDi19276.
    KEGGimmu:19276.
    UCSCiuc007phx.2. mouse. [P80560-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57345 mRNA. Translation: AAB06945.1 .
    U82439 mRNA. Translation: AAB39996.1 .
    BN000315
    , BN000293 , BN000294 , BN000295 , BN000296 , BN000297 , BN000298 , BN000299 , BN000300 , BN000301 , BN000302 , BN000303 , BN000304 , BN000305 , BN000306 , BN000307 , BN000308 , BN000309 , BN000310 , BN000311 , BN000312 , BN000313 , BN000314 Genomic DNA. Translation: CAG23871.1 .
    CCDSi CCDS36576.1. [P80560-1 ]
    RefSeqi NP_035345.2. NM_011215.2. [P80560-1 ]
    UniGenei Mm.206054.

    3D structure databases

    ProteinModelPortali P80560.
    SMRi P80560. Positions 497-583, 710-996.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P80560. 2 interactions.
    MINTi MINT-8310377.
    STRINGi 10090.ENSMUSP00000064046.

    PTM databases

    PhosphoSitei P80560.

    Proteomic databases

    MaxQBi P80560.
    PaxDbi P80560.
    PRIDEi P80560.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000070733 ; ENSMUSP00000064046 ; ENSMUSG00000056553 . [P80560-1 ]
    GeneIDi 19276.
    KEGGi mmu:19276.
    UCSCi uc007phx.2. mouse. [P80560-1 ]

    Organism-specific databases

    CTDi 5799.
    MGIi MGI:107418. Ptprn2.

    Phylogenomic databases

    eggNOGi COG5599.
    GeneTreei ENSGT00750000117312.
    HOGENOMi HOG000243992.
    HOVERGENi HBG105788.
    InParanoidi Q1RLJ1.
    KOi K07817.
    OMAi YRYEVSP.
    OrthoDBi EOG7K9K30.
    TreeFami TF351976.

    Miscellaneous databases

    NextBioi 296180.
    PROi P80560.
    SOURCEi Search...

    Gene expression databases

    Bgeei P80560.
    Genevestigatori P80560.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR021613. Receptor_IA-2_dom.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view ]
    Pfami PF11548. Receptor_IA-2. 1 hit.
    PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PTP-NP, a new member of the receptor protein tyrosine phosphatase family, implicated in development of nervous system and pancreatic endocrine cells."
      Chiang M.-K., Flanagan J.G.
      Development 122:2239-2250(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
      Tissue: Brain.
    2. "Identification of a second transmembrane protein tyrosine phosphatase, IA-2beta, as an autoantigen in insulin-dependent diabetes mellitus: precursor of the 37-kDa tryptic fragment."
      Lu J., Li Q., Xie H., Chen Z.-J., Borovitskaya A.E., Maclaren N.K., Notkins A.L., Lan M.S.
      Proc. Natl. Acad. Sci. U.S.A. 93:2307-2311(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 282-1001.
      Tissue: Neonatal brain.
    3. "Targeted disruption of the IA-2beta gene causes glucose intolerance and impairs insulin secretion but does not prevent the development of diabetes in NOD mice."
      Kubosaki A., Gross S., Miura J., Saeki K., Zhu M., Nakamura S., Hendriks W., Notkins A.L.
      Diabetes 53:1684-1691(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, DISRUPTION PHENOTYPE.
      Strain: C57BL/6.

    Entry informationi

    Entry nameiPTPR2_MOUSE
    AccessioniPrimary (citable) accession number: P80560
    Secondary accession number(s): O09134, P70328, Q1RLJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3