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P80560 (PTPR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Receptor-type tyrosine-protein phosphatase N2

Short name=R-PTP-N2
EC=3.1.3.48
Alternative name(s):
PTP IA-2beta
Protein tyrosine phosphatase-NP
Short name=PTP-NP
Gene names
Name:Ptprn2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1001 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in development of nervous system and pancreatic endocrine cells.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location

Cytoplasmic vesiclesecretory vesicle membrane; Single-pass type I membrane protein By similarity.

Tissue specificity

Pancreas and brain.

Developmental stage

Expressed in early stages of pancreatic development. First expressed in day 8.5 embryos (E8.5) in the dorsal part of the midgut endoderm and by E9.5, in the pancreatic rudiment specifically in early endocrine progenitor cells. At later stages expressed in insulin- or glucagon-producing cells. During neural development, the type 2 PTP-NP is expressed in early stages of neurogenesis, and the type 1 weakly in the later stages.

Post-translational modification

Appears to undergo multiple proteolytic cleavage at consecutive basic residues By similarity.

Involvement in disease

Autoantigen in insulin-dependent diabetes mellitus (IDDM).

Disruption phenotype

Mild glucose intolerance and impaired glucose-stimulated insulin secretion. Ref.3

Sequence similarities

Belongs to the protein-tyrosine phosphatase family.

Contains 1 tyrosine-protein phosphatase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PtprnQ606734EBI-8538944,EBI-8328895

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P80560-1)

Also known as: Type 1-PTP-NP;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P80560-2)

Also known as: Type 2-PTP-NP;

The sequence of this isoform is not available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 1001974Receptor-type tyrosine-protein phosphatase N2
PRO_0000025456

Regions

Topological domain28 – 600573Extracellular Potential
Transmembrane601 – 62121Helical; Potential
Topological domain622 – 1001380Cytoplasmic Potential
Domain731 – 991261Tyrosine-protein phosphatase
Region931 – 9377Substrate binding By similarity

Sites

Active site9311Phosphocysteine intermediate Potential
Binding site8991Substrate By similarity
Binding site9761Substrate By similarity
Site413 – 4142Cleavage By similarity

Amino acid modifications

Modified residue9561N6-acetyllysine By similarity
Glycosylation5501N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict1211A → T in AAB06945. Ref.1
Sequence conflict2661A → S in AAB06945. Ref.1
Sequence conflict3691E → D in AAB06945. Ref.1
Sequence conflict370 – 3712Missing in AAB39996. Ref.2
Sequence conflict3951P → H in AAB06945. Ref.1
Sequence conflict4111I → M in AAB39996. Ref.2
Sequence conflict4141S → L in AAB39996. Ref.2
Sequence conflict5861L → H in AAB39996. Ref.2
Sequence conflict6621I → V in AAB06945. Ref.1
Sequence conflict6621I → V in AAB39996. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Type 1-PTP-NP) [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 42B590A13CA89CB8

FASTA1,001111,497
        10         20         30         40         50         60 
MGPPLPLLLL LLLPPPLPRA LPAPASARGR QLPGRLGCLF EDGLCGSLET CVNDGVFGRC 

        70         80         90        100        110        120 
QKVPVMDTYR YEVPPGALLH LKVTLQKLSR TGFTWQDDYT QRVIAQELAN LPKAYLWHGE 

       130        140        150        160        170        180 
ASGPARSLQQ NADNEKWFSL EREVALAKTL RRYLPYLELL SQTPTANAHS RIDHETRPAK 

       190        200        210        220        230        240 
GEDSSPENIL TYVAHTSALT YPPATRAKYP DNLLRPFSRL QPDELSPKVD GDIDKQKLIA 

       250        260        270        280        290        300 
ALGAYTAQRL PGENDPEPRY LVHGSARAPR PFSATALSQR WPPPPGDAKD SPSMDDDTLL 

       310        320        330        340        350        360 
QSLLKDLQQN SEVDRLGPLK EEKADSVAGA IQSDPAEGSQ ESHGRGAEGQ PREQTDAPET 

       370        380        390        400        410        420 
MLQDHRLSEV DDPVYKEVNR LSFQLGDLLK DYGSPLLPEG PLLEKSSREE IKKSEQPEEV 

       430        440        450        460        470        480 
LSSEEETAGV EHVRSRTYSK DLFERKPNSE PQPRRLEDQF QNRAPELWED EESLKLAAQG 

       490        500        510        520        530        540 
PPSGGLQLEV QPSEEQQGYI LTGNNPLSPE KGKQLMDQVA HILRVPSSFF ADIKVLGPAV 

       550        560        570        580        590        600 
TFKVSANIQN MTTADVIKAA ADNKDQLEKA TGLTILQSGI RPKGKLKLLP HQEEQEDSTK 

       610        620        630        640        650        660 
FILLTFLSIA CILGVLLASS LAYCLRHNSH YKLKDKLSGL GADPSADATE AYQELCRQRM 

       670        680        690        700        710        720 
AIRPQDRSEG PHTSRINSVS SQFSDGPMPS PSARSSTSSW SEEPVQSNMD ISTGHMILAY 

       730        740        750        760        770        780 
MEDHLKNKNR LEKEWEALCA YQAEPNSSLV AQREENAPKN RSLAVLTYDH SRILLKSQNS 

       790        800        810        820        830        840 
HGSSDYINAS PIMDHDPRNP AYIATQGPLP ATVADFWQMV WESGCAVIVM LTPLSENGVR 

       850        860        870        880        890        900 
QCHHYWPDEG SNLYHVYEVN LVSEHIWCQD FLVRSFYLKN LQTNETRTVT QFHFLSWYDQ 

       910        920        930        940        950        960 
GVPSSTRSLL DFRRKVNKCY RGRSCPIIVH CSDGAGRSGT YVLIDMVLNK MAKGAKEIDI 

       970        980        990       1000 
AATLEHLRDQ RPGMVQTKEQ FEFALTAVAE EVNAILKALP Q 

« Hide

Isoform 2 (Type 2-PTP-NP) (Sequence not available).

References

[1]"PTP-NP, a new member of the receptor protein tyrosine phosphatase family, implicated in development of nervous system and pancreatic endocrine cells."
Chiang M.-K., Flanagan J.G.
Development 122:2239-2250(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
Tissue: Brain.
[2]"Identification of a second transmembrane protein tyrosine phosphatase, IA-2beta, as an autoantigen in insulin-dependent diabetes mellitus: precursor of the 37-kDa tryptic fragment."
Lu J., Li Q., Xie H., Chen Z.-J., Borovitskaya A.E., Maclaren N.K., Notkins A.L., Lan M.S.
Proc. Natl. Acad. Sci. U.S.A. 93:2307-2311(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 282-1001.
Tissue: Neonatal brain.
[3]"Targeted disruption of the IA-2beta gene causes glucose intolerance and impairs insulin secretion but does not prevent the development of diabetes in NOD mice."
Kubosaki A., Gross S., Miura J., Saeki K., Zhu M., Nakamura S., Hendriks W., Notkins A.L.
Diabetes 53:1684-1691(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, DISRUPTION PHENOTYPE.
Strain: C57BL/6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U57345 mRNA. Translation: AAB06945.1.
U82439 mRNA. Translation: AAB39996.1.
BN000315 expand/collapse EMBL AC list , BN000293, BN000294, BN000295, BN000296, BN000297, BN000298, BN000299, BN000300, BN000301, BN000302, BN000303, BN000304, BN000305, BN000306, BN000307, BN000308, BN000309, BN000310, BN000311, BN000312, BN000313, BN000314 Genomic DNA. Translation: CAG23871.1.
RefSeqNP_035345.2. NM_011215.2.
UniGeneMm.206054.

3D structure databases

ProteinModelPortalP80560.
SMRP80560. Positions 497-583, 710-996.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP80560. 2 interactions.
MINTMINT-8310377.
STRING10090.ENSMUSP00000064046.

PTM databases

PhosphoSiteP80560.

Proteomic databases

PaxDbP80560.
PRIDEP80560.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070733; ENSMUSP00000064046; ENSMUSG00000056553. [P80560-1]
GeneID19276.
KEGGmmu:19276.
UCSCuc007phx.2. mouse. [P80560-1]

Organism-specific databases

CTD5799.
MGIMGI:107418. Ptprn2.

Phylogenomic databases

eggNOGCOG5599.
GeneTreeENSGT00750000117312.
HOGENOMHOG000243992.
HOVERGENHBG105788.
InParanoidQ1RLJ1.
KOK07817.
OMAYRYEVSP.
OrthoDBEOG7K9K30.
TreeFamTF351976.

Gene expression databases

BgeeP80560.
GenevestigatorP80560.

Family and domain databases

InterProIPR021613. Receptor_IA-2.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamPF11548. Receptor_IA-2. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00700. PRTYPHPHTASE.
SMARTSM00194. PTPc. 1 hit.
[Graphical view]
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio296180.
PROP80560.
SOURCESearch...

Entry information

Entry namePTPR2_MOUSE
AccessionPrimary (citable) accession number: P80560
Secondary accession number(s): O09134, P70328, Q1RLJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot