Reviewed, UniProtKB/Swiss-Prot P80545 (BLAC_SERFO)

Names and origin

Protein names	Recommended name:     Beta-lactamase     EC=3.5.2.6 Alternative name(s):     Penicillinase Cleaved into the following 2 chains:     1- Recommended name:             Beta-lactamase form I     2- Recommended name:             Beta-lactamase form II
Organism	Serratia fonticola
Taxonomic identifier	47917 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Enzyme regulation	Inhibited by clavulanic acid, sulbactam and tazobactam.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Beta-lactamase form I		PRO_0000017010
	Chain	6 – 267	262	Beta-lactamase form II		PRO_0000017011
Regions
	Region	213 – 215	3	Substrate binding By similarity
Sites
	Active site	49	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80545-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: D70C1FDEE63F09C3	FASTA	267	28,755
	10 20 30 40 50 60 QPANAKANIQ QQLSELEKNS GGRLGVALID TADNSQILYR GDERFPMCST SKVMAVSALL 70 80 90 100 110 120 KQSETDKNLL AKRMEIKQSD LVNYNPIAEK HLDTGMTLAE FSAATIQYSD NTAMNKILEH 130 140 150 160 170 180 LGGPAKVTEF ARTIGDKTFR LDRTEPTLNT AIPGDKRDTT SPQAMAISLQ NLTLGKALAE 190 200 210 220 230 240 PQRAQLVEWM KGNTTGGASI RAGLPTTWVV GDKTGSGDYG TTNDIAVIWP ANHAPLVLVT 250 260 YFTQPQQNAE ARKDVLAAAA KIVTAGL

References

[1]

"Characterization and amino acid sequence analysis of a new oxyimino cephalosporin-hydrolyzing class A beta-lactamase from Serratia fonticola CUV." Peduzzi J., Farzaneh S., Reynaud A., Barthelemy M., Labia R. Biochim. Biophys. Acta 1341:58-70(1997) [PubMed: 9300809] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: CUV.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1BZA based on UniProtKB Q47066.
SMR	P80545. Positions 8-267.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 141797.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_SERFO

Accession

Primary (citable) accession number: P80545

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)