P80541 (MDH_STEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:

mdh

Organism

Stenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia)

Taxonomic identifier

40324 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Stenotrophomonas › Stenotrophomonas maltophilia group

Protein attributes

Sequence length	110 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›110	›110	Malate dehydrogenase HAMAP-Rule MF_01517		PRO_0000113404
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
Sites
	Binding site	94	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	107	1	NAD By similarity
Experimental info
	Non-terminal residue	110	1

Sequences

	Sequence		Length	Mass (Da)
	P80541 [UniParc]. Last modified June 7, 2004. Version 2. Checksum: 123924337D853F79	FASTA	110	11,619
	10 20 30 40 50 60 MKAPVRVAVT GAAGQIGYAL LFRIASGEML GKDQPVILQL LELPVDKAQA ALKGVMMELE 70 80 90 100 110 DCAFPLLAGM VGTDDAEVAF KDADIALLVG ARPRGPGMER KDLLLENAKI

References

[1]	"Gene cloning, overexpression and biochemical characterization of the peptide amidase from Stenotrophomonas maltophilia." Neumann S., Kula M.-R. Appl. Microbiol. Biotechnol. 58:772-780(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 9181.
[2]	"Structural studies of malate dehydrogenases (MDHs): MDHs in Brevundimonas species are the first reported MDHs in Proteobacteria which resemble lactate dehydrogenases in primary structure." Charnock C. J. Bacteriol. 179:4066-4070(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-22. Strain: ICPB 2648-67.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ414049 Genomic DNA. Translation: CAC93613.1.
3D structure databases
ProteinModelPortal	P80541.
SMR	P80541. Positions 2-110.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_01517. Malate_dehydrog_2.
InterPro	IPR001236. Lactate/malate_DH_N. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23382. PTHR23382. 1 hit.
Pfam	PF00056. Ldh_1_N. 1 hit. [Graphical view]
PROSITE	PS00068. MDH. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_STEMA

Accession

Primary (citable) accession number: P80541
Secondary accession number(s): Q8RJW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	June 7, 2004
Last modified:	April 3, 2013
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program