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P80536

- MDH_BURPS

UniProt

P80536 - MDH_BURPS

Protein

Malate dehydrogenase

Gene

mdh

Organism
Burkholderia pseudomallei (strain K96243)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931SubstrateBy similarity
    Binding sitei99 – 991SubstrateBy similarity
    Binding sitei106 – 1061NADBy similarity
    Binding sitei113 – 1131NADBy similarity
    Binding sitei132 – 1321SubstrateBy similarity
    Binding sitei163 – 1631SubstrateBy similarity
    Active sitei188 – 1881Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 187NADBy similarity
    Nucleotide bindingi130 – 1323NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciBPSE272560:GJNI-5293-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase (EC:1.1.1.37)
    Gene namesi
    Name:mdh
    Ordered Locus Names:BPSS1722
    OrganismiBurkholderia pseudomallei (strain K96243)
    Taxonomic identifieri272560 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiapseudomallei group
    ProteomesiUP000000605: Chromosome 2

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 327326Malate dehydrogenasePRO_0000113354Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi272560.BPSS1722.

    Structurei

    3D structure databases

    ProteinModelPortaliP80536.
    SMRiP80536. Positions 3-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000220953.
    KOiK00024.
    OMAiCALRDEV.
    OrthoDBiEOG6PP9Q2.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P80536-1 [UniParc]FASTAAdd to Basket

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    MAKPAKRVAV TGAAGQIAYS LLFRIANGDL LGKDQPVILQ LLDLPQAQAA    50
    VKGVVMELDD CAFPLLAGVV ITDDPKVAFK DADVALLVGA RPRSKGMERK 100
    DLLSANAEIF TVQGAALNEV ASRDVKVLVV GNPANTNAYI AMKSAPDLPK 150
    KNFTAMLRLD HNRALSQLAA KSGKPVASIE KLAVWGNHSP TMYPDFRFAT 200
    AEGESLLKLI NDDVWNRDTF IPTVGKRGAA IIEARGLSSA ASAANAAIDH 250
    VRDWVLGTNG KWVTMGIPSD GSYGIPEDII YGVPVICENG EYKRVEGLEI 300
    DAFSREKMDG TLAELLEERD GVAHLLK 327
    Length:327
    Mass (Da):35,015
    Last modified:January 23, 2007 - v3
    Checksum:i2E919ABA6D3F67B3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241R → K AA sequence (PubMed:9190829)Curated
    Sequence conflicti29 – 291D → K AA sequence (PubMed:9190829)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571966 Genomic DNA. Translation: CAH39196.1.
    RefSeqiYP_111728.1. NC_006351.1.

    Genome annotation databases

    EnsemblBacteriaiCAH39196; CAH39196; BPSS1722.
    GeneIDi3095126.
    KEGGibps:BPSS1722.
    PATRICi19271718. VBIBurPse99623_6030.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571966 Genomic DNA. Translation: CAH39196.1 .
    RefSeqi YP_111728.1. NC_006351.1.

    3D structure databases

    ProteinModelPortali P80536.
    SMRi P80536. Positions 3-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272560.BPSS1722.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAH39196 ; CAH39196 ; BPSS1722 .
    GeneIDi 3095126.
    KEGGi bps:BPSS1722.
    PATRICi 19271718. VBIBurPse99623_6030.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000220953.
    KOi K00024.
    OMAi CALRDEV.
    OrthoDBi EOG6PP9Q2.

    Enzyme and pathway databases

    BioCyci BPSE272560:GJNI-5293-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01517. Malate_dehydrog_2.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K96243.
    2. "Structural studies of malate dehydrogenases (MDHs): MDHs in Brevundimonas species are the first reported MDHs in Proteobacteria which resemble lactate dehydrogenases in primary structure."
      Charnock C.
      J. Bacteriol. 179:4066-4070(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-30.
      Strain: ATCC 23343.

    Entry informationi

    Entry nameiMDH_BURPS
    AccessioniPrimary (citable) accession number: P80536
    Secondary accession number(s): Q63JJ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3