Reviewed, UniProtKB/Swiss-Prot P80534 (G3P1_JACOR)

Names and origin

Protein names	Recommended name:     Glyceraldehyde-3-phosphate dehydrogenase, muscle       Short name=GAPDH     EC=1.2.1.12
Organism	Jaculus orientalis (Greater Egyptian jerboa)
Taxonomic identifier	48868 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Dipodidae › Dipodinae › Jaculus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Post-translational modification	Reversible S-nitrosylation of Cys-180 inhibits enzymatic activity and increases endogenous ADP-ribosylation, which inhibits the enzyme in a non-reversible manner. The latter modification is more likely to be a pathophysiological event associated with inhibition of gluconeogenesis By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	ADP-ribosylation S-nitrosylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 363	362	Glyceraldehyde-3-phosphate dehydrogenase, muscle		PRO_0000145487
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	179 – 181	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	239 – 240	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	180	1	Nucleophile By similarity
	Binding site	63	1	NAD By similarity
	Binding site	108	1	NAD; via carbonyl oxygen By similarity
	Binding site	210	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	262	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	344	1	NAD By similarity
	Site	207	1	Activates thiol group during catalysis By similarity
Amino acid modifications
	Modified residue	180	1	ADP-ribosylcysteine; by autocatalysis; in irreversibly inhibited form By similarity
	Modified residue	180	1	S-nitrosocysteine; in reversibly inhibited form By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80534-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 6CB549D9A1163C3F	FASTA	363	39,413
	10 20 30 40 50 60 MVKVGVNGFG RIGRLVTRAA FNSGKVDIVA INDPFIDLNY MVYMFKYDST HGKFKVDIVA 70 80 90 100 110 120 INDPFIDLNY MVYMFKYDST HGKFKGTVKA ENGKLVINGH AITIFQERDP SKIKWGDAGA 130 140 150 160 170 180 EYVVESTGVF TTMEKAGAHL KGGAKRVIIS APSRDAPMFV MGVNHEKYDK SLKIVSNASC 190 200 210 220 230 240 TTNCLAPLAK VIHDNFGIVE GLMTTVHAIT ATQKTVDGPS AKLWRDGAGA AQNIIPASTG 250 260 270 280 290 300 AAKAVGKVIP ELNGKLTGMA FRVPTANVSV VDLTCRLEKP AKYDDIKRVV KQACDGPLKG 310 320 330 340 350 360 MLGYTEHQVV SSDFNGDSHS STFDAGAGIA LNDHFVKLVS WYDNEFGYSN RVVDLMVHMA SKE

References

[1]	Soukri A., Serrano A. Submitted (JAN-1996) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-54. Tissue: Skeletal muscle.
[2]	"Occurrence of a differential expression of the glyceraldehyde-3-phosphate dehydrogenase gene in muscle and liver from euthermic and induced hibernating jerboa (Jaculus orientalis)." Soukri A., Valverde F., Hafid N., Elkebbaj M.S., Serrano A. Gene 181:139-145(1996) [PubMed: 8973322] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-363. Tissue: Skeletal muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X87226 mRNA. Translation: CAA60678.1.
PIR	JC5370.
3D structure databases
HSSP	HSSP built from PDB template 3GPD based on UniProtKB P00354.
SMR	P80534. Positions 31-363.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P80534.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P1_JACOR

Accession

Primary (citable) accession number: P80534
Secondary accession number(s): Q64418

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)