Reviewed, UniProtKB/Swiss-Prot P80522 (PORB_METBF)

Names and origin

Protein names

Recommended name:
    Pyruvate synthase subunit porB
    EC=1.2.7.1
Alternative name(s):
    Pyruvate oxidoreductase beta chain
      Short name=POR
    Pyruvic-ferredoxin oxidoreductase subunit beta

Gene names

Name:	porB
Ordered Locus Names:	Mbar_A0999

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Pyruvate + CoA + 2 oxidized ferredoxin = acetyl-CoA + CO(2) + 2 reduced ferredoxin + 2 H(+).
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain.
Miscellaneous	It also catalyzes the oxidation of 2-oxobutyrate.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	pyruvate synthase activity Inferred from electronic annotation. Source: EC thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 296	295	Pyruvate synthase subunit porB		PRO_0000097126

Sequences

	Sequence		Length	Mass (Da)
	P80522-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C040A6216787AC14	FASTA	296	32,056
	10 20 30 40 50 60 MSKTAPKTYI TSGHSGCAGC CDAFAAKFTL MGAGPNTIVI NPTGCLEVMS TPFPYSSWQV 70 80 90 100 110 120 PWIHSLFENA GAVASGVEAA LKALGKKDDV KVVSIGGDGS TMDIGLGALS GAFERGHDFT 130 140 150 160 170 180 YVCMDNEAYM NTGVQRSSGT PFDASTTTTP AGKVSFGNPR PKKNMPAIMA AHGSPYVATT 190 200 210 220 230 240 SIGFPRDMIR KVKKATEIVG PTYIHAQAPC PTGWGFDTSK TLEIAKLAVE TCLWPMYEME 250 260 270 280 290 NGEITQVRKV KNPRPVEEYL RAQKRFKHLF TMEGGEEEIK KIQAIADWNI KHFELQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Catalytic properties, molecular composition and sequence alignments of pyruvate: ferredoxin oxidoreductase from the methanogenic archaeon Methanosarcina barkeri (strain Fusaro)." Bock A.-K., Kunow J., Glasemacher J., Schoenheit P. Eur. J. Biochem. 237:35-44(1996) [PubMed: 8620891] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-35.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000099 Genomic DNA. Translation: AAZ69967.1.
PIR	S65417.
RefSeq	YP_304547.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3626882.
GenomeReviews	Gene locus Mbar_A0999 in contig CP000099_GR.
KEGG	mba:Mbar_A0999.
NMPDR	fig|269797.3.peg.1010.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P80522.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0999-MON.
Family and domain databases
InterPro	IPR011766. TPP_enzyme_bd_C. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PORB_METBF

Accession

Primary (citable) accession number: P80522
Secondary accession number(s): Q46DS5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 45 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)