P80521 (PORA_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate synthase subunit porA
EC=1.2.7.1

Alternative name(s):
Pyruvate oxidoreductase alpha chain
Short name=POR
Pyruvic-ferredoxin oxidoreductase subunit alpha

Gene names

Name:	porA
Ordered Locus Names:	Mbar_A1000

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Pyruvate + CoA + 2 oxidized ferredoxin = acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+.
Subunit structure	Heterotetramer of one alpha, one beta, one delta and one gamma chain.
Miscellaneous	It also catalyzes the oxidation of 2-oxobutyrate.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Molecular function	pyruvate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	Pyruvate synthase subunit porA		PRO_0000097125

Sequences

	Sequence		Length	Mass (Da)
	P80521 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: D7DA8CA3AF2143B6	FASTA	402	44,503
	10 20 30 40 50 60 MIDPAYRKKM VVVEGSYAVA HSAKVCRPNV ISAYPITPQT HIVEHLSQFM ADGEIPNCEY 70 80 90 100 110 120 VNVEAEFSAI SALIGASAVG ARTYSATTSQ GLLLMHEALF NTSGMRLPVV MTVANRAVSA 130 140 150 160 170 180 PINIWNDHQD AIAQRDTGWM QLYVEDVQEA CDTLPQLYKI AEDNEIMVPG MVCMDGFILS 190 200 210 220 230 240 HVYEPVVLLE QDLTDNFLPP FQPEDILDPE DPKTFGAFAS PDTYEEFRYL HEQAMQKALP 250 260 270 280 290 300 KIEATAKEFE EVYGRYHGGL IDGYMLDDAE IVVMAMGSIL GTVKDVVDKY RAKGEKIGVL 310 320 330 340 350 360 KVRSFRPFPK EQICKAVKNA HAVVVLDKNI SIGTNEGALF TETKSCLYNS KVRVPVIGYT 370 380 390 400 IGHGGRDIPV ESIAKVIEET KKVAKSGITI ESQFMDLKEE LL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.
[2]	"Catalytic properties, molecular composition and sequence alignments of pyruvate: ferredoxin oxidoreductase from the methanogenic archaeon Methanosarcina barkeri (strain Fusaro)." Bock A.-K., Kunow J., Glasemacher J., Schoenheit P. Eur. J. Biochem. 237:35-44(1996) [PubMed: 8620891] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-38.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000099 Genomic DNA. Translation: AAZ69968.1.
PIR	S65416.
RefSeq	YP_304548.1. NC_007355.1.
3D structure databases
ProteinModelPortal	P80521.
ModBase	Search...
Protein-protein interaction databases
STRING	P80521.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3626883.
GenomeReviews	Gene locus Mbar_A1000 in contig CP000099_GR.
KEGG	mba:Mbar_A1000.
NMPDR	fig|269797.3.peg.1011.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04346.
HOGENOM	HBG313202.
OMA	FTETKSC.
ProtClustDB	CLSK811631.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A1000-MONOMER.
Family and domain databases
InterPro	IPR002880. Pyrv_Fd/Flavodoxin_OxRdtase_N. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005476. Transketolase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K00169.
Pfam	PF01855. POR_N. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PORA_METBF

Accession

Primary (citable) accession number: P80521
Secondary accession number(s): Q46DS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 10, 2006
Last modified:	November 16, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program