P80513 (MANF_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mesencephalic astrocyte-derived neurotrophic factor

Alternative name(s):
Arginine-rich protein
Protein ARMET

Gene names

Name:	MANF
Synonyms:	ARMET, ARP

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death By similarity.
Subcellular location	Secreted By similarity.
Domain	The N-terminal region may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response By similarity.
Sequence similarities	Belongs to the ARMET family.

Ontologies

Keywords
Biological process	Unfolded protein response
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	response to unfolded protein Inferred from electronic annotation. Source: UniProtKB-KW vasoconstriction of artery involved in ischemic response to lowering of systemic arterial blood pressure Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.2 Ref.3
	Chain	22 – 179	158	Mesencephalic astrocyte-derived neurotrophic factor		PRO_0000221218
Amino acid modifications
	Modified residue	4	1	Phosphothreonine By similarity
	Modified residue	14	1	Phosphoserine By similarity
	Modified residue	73	1	Phosphotyrosine By similarity
	Disulfide bond	27 ↔ 114		By similarity
	Disulfide bond	30 ↔ 103		By similarity
	Disulfide bond	61 ↔ 72		By similarity
	Disulfide bond	148 ↔ 151		By similarity
Experimental info
	Sequence conflict	27	1	C → T AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P80513 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 183F89FE5560A7FC	FASTA	179	20,357
	10 20 30 40 50 60 MWATHGLAVA LALSVLPASR ALRQGDCEVC ISYLGRFYQD LKDRDVTFSP ASIEKELIKF 70 80 90 100 110 120 CREARGKENR LCYYIGATED AATKIINEVS KPLSHHIPVE KICEKLKKKD SQICELKYDK 130 140 150 160 170 QIDLSTVDLK KLRVKELKKI LDDWGETCKG CAEKSDYIRK INELMPKYAP KAASSRTDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.
[2]	"Proteins and their amino acid compositions: uniqueness, variability, and applications." Galat A., Gerbod M.C., Bouet F., Riviere S. Arch. Biochem. Biophys. 330:229-237(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-60. Tissue: Kidney.
[3]	"ARMET is a soluble ER protein induced by the unfolded protein response via ERSE-II element." Mizobuchi N., Hoseki J., Kubota H., Toyokuni S., Nozaki J., Naitoh M., Koizumi A., Nagata K. Cell Struct. Funct. 32:41-50(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-31.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC149246 mRNA. Translation: AAI49247.1.
IPI	IPI00697196.
PIR	S69268.
RefSeq	NP_001094681.1. NM_001101211.2.
UniGene	Bt.10236.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P80513.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000045083; ENSBTAP00000042500; ENSBTAG00000031797.
GeneID	540432.
KEGG	bta:540432.
Organism-specific databases
CTD	7873.
Phylogenomic databases
eggNOG	NOG301491.
GeneTree	ENSGT00390000007160.
HOGENOM	HOG000264245.
InParanoid	P80513.
OMA	ESCKGCA.
OrthoDB	EOG4VT5Z8.
Family and domain databases
Gene3D	1.10.225.10. 1 hit.
InterPro	IPR019345. Armet_prot. IPR011001. Saposin-like. [Graphical view]
Pfam	PF10208. Armet. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20878634.

Entry information

Entry name

MANF_BOVIN

Accession

Primary (citable) accession number: P80513
Secondary accession number(s): A6QPB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 9, 2007
Last modified:	April 3, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program