Reviewed, UniProtKB/Swiss-Prot P80506 (G3P1_ANASP)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 1
      Short name=GAPDH 1
    EC=1.2.1.12

Gene names

Name:	gap1
Ordered Locus Names:	all2566

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 343	342	Glyceraldehyde-3-phosphate dehydrogenase 1		PRO_0000145623
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	154 – 156	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	214 – 215	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	155	1	Nucleophile By similarity
	Binding site	35	1	NAD By similarity
	Binding site	79	1	NAD; via carbonyl oxygen By similarity
	Binding site	185	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	237	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	319	1	NAD By similarity
	Site	182	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80506-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 0DA536A1C2728777	FASTA	343	36,752
	10 20 30 40 50 60 MAKLKVGING FGRIGRLVLR AGINNPNIEF VGINDLVPPD NLAYLLKYDS THGRLRSQVE 70 80 90 100 110 120 TKDDGIVIDG HFIPCVSVRN PAELPWGKLG ADYVVESTGL FTDSEGASKH LQAGARRVII 130 140 150 160 170 180 SAPTKDPDRV RTLLVGVNHD LFDPSKDLIV SNASCTTNCL APIAKVINDN FGLTEGLMTT 190 200 210 220 230 240 VHAMTATQPT VDGPSKKDWR GGRGAAQNII PSSTGAAKAV ALVLPELKGK LTGMAFRVPT 250 260 270 280 290 300 PDVSVVDLTF KTAKATSYKE ICAAMKQASE GSLAGILGYT DEEVVSTDFQ GDTHSSIFDA 310 320 330 340 GAGIELNSNF FKVVAWYDNE WGYSNRVVDL MLSMVQKEQL AAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	Valverde F., Serrano A. Submitted (NOV-1995) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-36.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB74265.1.
PIR	AG2126.
RefSeq	NP_486606.1.
3D structure databases
HSSP	HSSP built from PDB template 1DC5 based on UniProtKB P06977.
ModBase	Search...
Genome annotation databases
GeneID	1106163.
GenomeReviews	Gene locus all2566 in contig BA000019_GR.
KEGG	ana:all2566.
NMPDR	fig|103690.1.peg.2873.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P80506.
Enzyme and pathway databases
BioCyc	NSP103690:ALL2566-MON.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

G3P1_ANASP

Accession

Primary (citable) accession number: P80506

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 4, 2008
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)