Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glyceraldehyde-3-phosphate dehydrogenase 1

Gene

gap1

Organism
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.By similarity

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Curated
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (gap1), Glyceraldehyde-3-phosphate dehydrogenase 3 (gap3)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (all4008), Pyruvate kinase (all2564)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351NADBy similarity
Binding sitei79 – 791NAD; via carbonyl oxygenBy similarity
Binding sitei121 – 1211NADBy similarity
Active sitei155 – 1551NucleophileBy similarity
Sitei182 – 1821Activates thiol group during catalysisBy similarity
Binding sitei185 – 1851Glyceraldehyde 3-phosphateBy similarity
Binding sitei237 – 2371Glyceraldehyde 3-phosphateBy similarity
Binding sitei319 – 3191NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 142NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 11 Publication (EC:1.2.1.12By similarity)
Short name:
GAPDH 11 Publication
Alternative name(s):
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase1 Publication
Gene namesi
Name:gap1
Ordered Locus Names:all2566
OrganismiNostoc sp. (strain PCC 7120 / UTEX 2576)
Taxonomic identifieri103690 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000002483 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 343342Glyceraldehyde-3-phosphate dehydrogenase 1PRO_0000145623Add
BLAST

Proteomic databases

PRIDEiP80506.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi103690.all2566.

Structurei

3D structure databases

ProteinModelPortaliP80506.
SMRiP80506. Positions 3-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni154 – 1563Glyceraldehyde 3-phosphate bindingBy similarity
Regioni214 – 2152Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C17. Bacteria.
COG0057. LUCA.
HOGENOMiHOG000071678.
KOiK00134.
OMAiKWGEVGA.
OrthoDBiEOG66TG3S.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLKVGING FGRIGRLVLR AGINNPNIEF VGINDLVPPD NLAYLLKYDS
60 70 80 90 100
THGRLRSQVE TKDDGIVIDG HFIPCVSVRN PAELPWGKLG ADYVVESTGL
110 120 130 140 150
FTDSEGASKH LQAGARRVII SAPTKDPDRV RTLLVGVNHD LFDPSKDLIV
160 170 180 190 200
SNASCTTNCL APIAKVINDN FGLTEGLMTT VHAMTATQPT VDGPSKKDWR
210 220 230 240 250
GGRGAAQNII PSSTGAAKAV ALVLPELKGK LTGMAFRVPT PDVSVVDLTF
260 270 280 290 300
KTAKATSYKE ICAAMKQASE GSLAGILGYT DEEVVSTDFQ GDTHSSIFDA
310 320 330 340
GAGIELNSNF FKVVAWYDNE WGYSNRVVDL MLSMVQKEQL AAV
Length:343
Mass (Da):36,752
Last modified:January 23, 2007 - v3
Checksum:i0DA536A1C2728777
GO

Mass spectrometryi

Molecular mass is 36850 Da from positions 1 - 343. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB74265.1.
AJ251773 Genomic DNA. Translation: CAC41000.1.
PIRiAG2126.
RefSeqiWP_010996722.1. NC_003272.1.

Genome annotation databases

EnsemblBacteriaiBAB74265; BAB74265; BAB74265.
KEGGiana:all2566.
PATRICi22775340. VBINosSp37423_3135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000019 Genomic DNA. Translation: BAB74265.1.
AJ251773 Genomic DNA. Translation: CAC41000.1.
PIRiAG2126.
RefSeqiWP_010996722.1. NC_003272.1.

3D structure databases

ProteinModelPortaliP80506.
SMRiP80506. Positions 3-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi103690.all2566.

Proteomic databases

PRIDEiP80506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB74265; BAB74265; BAB74265.
KEGGiana:all2566.
PATRICi22775340. VBINosSp37423_3135.

Phylogenomic databases

eggNOGiENOG4105C17. Bacteria.
COG0057. LUCA.
HOGENOMiHOG000071678.
KOiK00134.
OMAiKWGEVGA.
OrthoDBiEOG66TG3S.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7120 / UTEX 2576.
  2. "Simultaneous occurrence of two different glyceraldehyde-3-phosphate dehydrogenases in heterocystous N(2)-fixing cyanobacteria."
    Valverde F., Peleato M.L., Fillat M.F., Gomez-Moreno C., Losada M., Serrano A.
    Biochem. Biophys. Res. Commun. 283:356-363(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-36, NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 9-322, FUNCTION, MASS SPECTROMETRY, SUBUNIT.
    Strain: PCC 7120 / UTEX 2576.

Entry informationi

Entry nameiG3P1_NOSS1
AccessioniPrimary (citable) accession number: P80506
Secondary accession number(s): Q93M83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: December 9, 2015
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

No significant Gap1 activity is detected in cells under different growth conditions. Gap3 is certainly the main GAPDH present in cells.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.