Reviewed, UniProtKB/Swiss-Prot P80491 (FRHG_METBF)

Names and origin

Protein names

Recommended name:
    Coenzyme F420 hydrogenase subunit gamma
    EC=1.12.98.1
Alternative name(s):
    8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
      Short name=FRH

Gene names

Name:	frhG
Ordered Locus Names:	Mbar_A0450

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.
Catalytic activity	H(2) + coenzyme F420 = reduced coenzyme F420.
Cofactor	Nickel. Iron-sulfur. FAD.
Subunit structure	Pentamer of two alpha chains, two beta chains and a gamma chain.
Sequence similarities	Belongs to the frhG family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 259	258	Coenzyme F420 hydrogenase subunit gamma		PRO_0000159231
Regions
	Domain	180 – 208	29	4Fe-4S ferredoxin-type 1
	Domain	209 – 238	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	189	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	192	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	195	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	199	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	218	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	221	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	224	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	228	1	Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80491-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 46C4AE265B6146BF	FASTA	259	28,241
	10 20 30 40 50 60 MTNKIKIGHV HMSGCTGCLV SLADNNLGLI KILDDYADLV YCLTLADVRH IPEMDVALVE 70 80 90 100 110 120 GSVCLQDHES VEDIKETRKK SKIVVALGSC ACYGNITRFS RGGQHNQPQH ESYLPIGDLI 130 140 150 160 170 180 DVDVYIPGCP PSPELIRNVA VMAYLLLEGN EEQKELAGKY LKPLMDLAKR GTSGCFCDLM 190 200 210 220 230 240 YDVINQGLCM GCGTCAASCP VHAITLEFGK PQGERDLCIK CGSCYGACPR SFFNLDVIPE 250 FENINEIIAN ALKEGESDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two F420-reducing hydrogenases in Methanosarcina barkeri." Vaupel M., Thauer R.K. Arch. Microbiol. 169:201-205(1998) [PubMed: 9477253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro." Michel R., Massanz C., Kostka S., Richter M., Fiebig K. Eur. J. Biochem. 233:727-735(1995) [PubMed: 8521835] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-20.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y13763 Genomic DNA. Translation: CAA74092.1. CP000099 Genomic DNA. Translation: AAZ69432.1. Different initiation.
PIR	S63485.
RefSeq	YP_304012.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3624326.
GenomeReviews	Gene locus Mbar_A0450 in contig CP000099_GR.
KEGG	mba:Mbar_A0450.
NMPDR	fig|269797.3.peg.628.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P80491.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0450-MON. MetaCyc:MON-12649.
Family and domain databases
InterPro	IPR001450. 4Fe4S_Fe_S_bd. IPR017681. Coenz_F4210_hydrogenase_gsu. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. IPR006137. OxRdtase_q6. [Graphical view]
PANTHER	PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF00037. Fer4. 2 hits. PF01058. Oxidored_q6. 1 hit. [Graphical view]
PRINTS	PR00353. 4FE4SFRDOXIN.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRHG_METBF

Accession

Primary (citable) accession number: P80491
Secondary accession number(s): O33167, Q46FB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 51 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)