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Protein

Coenzyme F420 hydrogenase subunit gamma

Gene

frhG

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.

Catalytic activityi

H2 + oxidized coenzyme F420 = reduced coenzyme F420.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi189 – 1891Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi192 – 1921Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi195 – 1951Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi199 – 1991Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi218 – 2181Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi221 – 2211Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi224 – 2241Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi228 – 2281Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. coenzyme F420 hydrogenase activity Source: UniProtKB-EC
  3. flavin adenine dinucleotide binding Source: InterPro
  4. nickel cation binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-461-MONOMER.
MetaCyc:MONOMER-12649.
BRENDAi1.12.98.1. 3250.

Names & Taxonomyi

Protein namesi
Recommended name:
Coenzyme F420 hydrogenase subunit gamma (EC:1.12.98.1)
Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
Short name:
FRH
Gene namesi
Name:frhG
Ordered Locus Names:Mbar_A0450
OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
Taxonomic identifieri269797 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000008156: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 259258Coenzyme F420 hydrogenase subunit gammaPRO_0000159231Add
BLAST

Interactioni

Subunit structurei

Pentamer of two alpha chains, two beta chains and a gamma chain.

Protein-protein interaction databases

STRINGi269797.Mbar_A0450.

Structurei

3D structure databases

ProteinModelPortaliP80491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini180 – 208294Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini209 – 238304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the FrhG family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1941.
HOGENOMiHOG000236993.
KOiK00443.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017681. Coenz_F420_hydrogenase_gsu.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
[Graphical view]
PfamiPF13187. Fer4_9. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03294. FrhG. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80491-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNKIKIGHV HMSGCTGCLV SLADNNLGLI KILDDYADLV YCLTLADVRH
60 70 80 90 100
IPEMDVALVE GSVCLQDHES VEDIKETRKK SKIVVALGSC ACYGNITRFS
110 120 130 140 150
RGGQHNQPQH ESYLPIGDLI DVDVYIPGCP PSPELIRNVA VMAYLLLEGN
160 170 180 190 200
EEQKELAGKY LKPLMDLAKR GTSGCFCDLM YDVINQGLCM GCGTCAASCP
210 220 230 240 250
VHAITLEFGK PQGERDLCIK CGSCYGACPR SFFNLDVIPE FENINEIIAN

ALKEGESDD
Length:259
Mass (Da):28,241
Last modified:January 23, 2007 - v3
Checksum:i46C4AE265B6146BF
GO

Sequence cautioni

The sequence AAZ69432.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13763 Genomic DNA. Translation: CAA74092.1.
CP000099 Genomic DNA. Translation: AAZ69432.1. Different initiation.
PIRiS63485.
RefSeqiYP_304012.1. NC_007355.1.

Genome annotation databases

EnsemblBacteriaiAAZ69432; AAZ69432; Mbar_A0450.
GeneIDi3624326.
KEGGimba:Mbar_A0450.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13763 Genomic DNA. Translation: CAA74092.1.
CP000099 Genomic DNA. Translation: AAZ69432.1. Different initiation.
PIRiS63485.
RefSeqiYP_304012.1. NC_007355.1.

3D structure databases

ProteinModelPortaliP80491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269797.Mbar_A0450.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ69432; AAZ69432; Mbar_A0450.
GeneIDi3624326.
KEGGimba:Mbar_A0450.

Phylogenomic databases

eggNOGiCOG1941.
HOGENOMiHOG000236993.
KOiK00443.

Enzyme and pathway databases

BioCyciMBAR269797:GHUW-461-MONOMER.
MetaCyc:MONOMER-12649.
BRENDAi1.12.98.1. 3250.

Family and domain databases

Gene3Di3.40.50.700. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017681. Coenz_F420_hydrogenase_gsu.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
[Graphical view]
PfamiPF13187. Fer4_9. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03294. FrhG. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Two F420-reducing hydrogenases in Methanosarcina barkeri."
    Vaupel M., Thauer R.K.
    Arch. Microbiol. 169:201-205(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
    Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
    J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fusaro / DSM 804.
  3. "Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro."
    Michel R., Massanz C., Kostka S., Richter M., Fiebig K.
    Eur. J. Biochem. 233:727-735(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-20.

Entry informationi

Entry nameiFRHG_METBF
AccessioniPrimary (citable) accession number: P80491
Secondary accession number(s): O33167, Q46FB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.