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P80491 (FRHG_METBF) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Coenzyme F420 hydrogenase subunit gamma

EC=1.12.98.1
Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
Short name=FRH
Gene names
Name:frhG
Ordered Locus Names:Mbar_A0450
OrganismMethanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]
Taxonomic identifier269797 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.

Catalytic activity

H2 + oxidized coenzyme F420 = reduced coenzyme F420.

Cofactor

Nickel.

Iron-sulfur.

FAD.

Subunit structure

Pentamer of two alpha chains, two beta chains and a gamma chain.

Sequence similarities

Belongs to the FrhG family.

Contains 2 4Fe-4S ferredoxin-type domains.

Sequence caution

The sequence AAZ69432.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.3
Chain2 – 259258Coenzyme F420 hydrogenase subunit gamma
PRO_0000159231

Regions

Domain180 – 208294Fe-4S ferredoxin-type 1
Domain209 – 238304Fe-4S ferredoxin-type 2

Sites

Metal binding1891Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1921Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1951Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1991Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2181Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2211Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2241Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2281Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P80491 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 46C4AE265B6146BF

FASTA25928,241
        10         20         30         40         50         60 
MTNKIKIGHV HMSGCTGCLV SLADNNLGLI KILDDYADLV YCLTLADVRH IPEMDVALVE 

        70         80         90        100        110        120 
GSVCLQDHES VEDIKETRKK SKIVVALGSC ACYGNITRFS RGGQHNQPQH ESYLPIGDLI 

       130        140        150        160        170        180 
DVDVYIPGCP PSPELIRNVA VMAYLLLEGN EEQKELAGKY LKPLMDLAKR GTSGCFCDLM 

       190        200        210        220        230        240 
YDVINQGLCM GCGTCAASCP VHAITLEFGK PQGERDLCIK CGSCYGACPR SFFNLDVIPE 

       250 
FENINEIIAN ALKEGESDD 

« Hide

References

« Hide 'large scale' references
[1]"Two F420-reducing hydrogenases in Methanosarcina barkeri."
Vaupel M., Thauer R.K.
Arch. Microbiol. 169:201-205(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fusaro / DSM 804.
[3]"Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro."
Michel R., Massanz C., Kostka S., Richter M., Fiebig K.
Eur. J. Biochem. 233:727-735(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-20.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y13763 Genomic DNA. Translation: CAA74092.1.
CP000099 Genomic DNA. Translation: AAZ69432.1. Different initiation.
PIRS63485.
RefSeqYP_304012.1. NC_007355.1.

3D structure databases

ProteinModelPortalP80491.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269797.Mbar_A0450.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ69432; AAZ69432; Mbar_A0450.
GeneID3624326.
KEGGmba:Mbar_A0450.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1941.
HOGENOMHOG000236993.
KOK00443.
ProtClustDBCLSK876042.

Enzyme and pathway databases

BioCycMBAR269797:GHUW-461-MONOMER.
MetaCyc:MONOMER-12649.
BRENDA1.12.98.1. 3250.

Family and domain databases

Gene3D3.40.50.700. 1 hit.
InterProIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR017681. Coenz_F420_hydrogenase_gsu.
IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
[Graphical view]
PfamPF13187. Fer4_9. 1 hit.
PF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsTIGR03294. FrhG. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFRHG_METBF
AccessionPrimary (citable) accession number: P80491
Secondary accession number(s): O33167, Q46FB0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 86 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families