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P80491

- FRHG_METBF

UniProt

P80491 - FRHG_METBF

Protein

Coenzyme F420 hydrogenase subunit gamma

Gene

frhG

Organism
Methanosarcina barkeri (strain Fusaro / DSM 804)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.

    Catalytic activityi

    H2 + oxidized coenzyme F420 = reduced coenzyme F420.

    Cofactori

    Nickel.
    Iron-sulfur.
    FAD.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi189 – 1891Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi192 – 1921Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi195 – 1951Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi199 – 1991Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi218 – 2181Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi221 – 2211Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi224 – 2241Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi228 – 2281Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. coenzyme F420 hydrogenase activity Source: UniProtKB-EC
    3. flavin adenine dinucleotide binding Source: InterPro
    4. nickel cation binding Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciMBAR269797:GHUW-461-MONOMER.
    MetaCyc:MONOMER-12649.
    BRENDAi1.12.98.1. 3250.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Coenzyme F420 hydrogenase subunit gamma (EC:1.12.98.1)
    Alternative name(s):
    8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma
    Short name:
    FRH
    Gene namesi
    Name:frhG
    Ordered Locus Names:Mbar_A0450
    OrganismiMethanosarcina barkeri (strain Fusaro / DSM 804)
    Taxonomic identifieri269797 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000008156: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 259258Coenzyme F420 hydrogenase subunit gammaPRO_0000159231Add
    BLAST

    Interactioni

    Subunit structurei

    Pentamer of two alpha chains, two beta chains and a gamma chain.

    Protein-protein interaction databases

    STRINGi269797.Mbar_A0450.

    Structurei

    3D structure databases

    ProteinModelPortaliP80491.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini180 – 208294Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini209 – 238304Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the FrhG family.Curated
    Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1941.
    HOGENOMiHOG000236993.
    KOiK00443.

    Family and domain databases

    Gene3Di3.40.50.700. 1 hit.
    InterProiIPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR017681. Coenz_F420_hydrogenase_gsu.
    IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    [Graphical view]
    PfamiPF13187. Fer4_9. 1 hit.
    PF01058. Oxidored_q6. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03294. FrhG. 1 hit.
    PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P80491-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTNKIKIGHV HMSGCTGCLV SLADNNLGLI KILDDYADLV YCLTLADVRH    50
    IPEMDVALVE GSVCLQDHES VEDIKETRKK SKIVVALGSC ACYGNITRFS 100
    RGGQHNQPQH ESYLPIGDLI DVDVYIPGCP PSPELIRNVA VMAYLLLEGN 150
    EEQKELAGKY LKPLMDLAKR GTSGCFCDLM YDVINQGLCM GCGTCAASCP 200
    VHAITLEFGK PQGERDLCIK CGSCYGACPR SFFNLDVIPE FENINEIIAN 250
    ALKEGESDD 259
    Length:259
    Mass (Da):28,241
    Last modified:January 23, 2007 - v3
    Checksum:i46C4AE265B6146BF
    GO

    Sequence cautioni

    The sequence AAZ69432.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13763 Genomic DNA. Translation: CAA74092.1.
    CP000099 Genomic DNA. Translation: AAZ69432.1. Different initiation.
    PIRiS63485.
    RefSeqiYP_304012.1. NC_007355.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ69432; AAZ69432; Mbar_A0450.
    GeneIDi3624326.
    KEGGimba:Mbar_A0450.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y13763 Genomic DNA. Translation: CAA74092.1 .
    CP000099 Genomic DNA. Translation: AAZ69432.1 . Different initiation.
    PIRi S63485.
    RefSeqi YP_304012.1. NC_007355.1.

    3D structure databases

    ProteinModelPortali P80491.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269797.Mbar_A0450.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ69432 ; AAZ69432 ; Mbar_A0450 .
    GeneIDi 3624326.
    KEGGi mba:Mbar_A0450.

    Phylogenomic databases

    eggNOGi COG1941.
    HOGENOMi HOG000236993.
    KOi K00443.

    Enzyme and pathway databases

    BioCyci MBAR269797:GHUW-461-MONOMER.
    MetaCyc:MONOMER-12649.
    BRENDAi 1.12.98.1. 3250.

    Family and domain databases

    Gene3Di 3.40.50.700. 1 hit.
    InterProi IPR017896. 4Fe4S_Fe-S-bd.
    IPR017900. 4Fe4S_Fe_S_CS.
    IPR017681. Coenz_F420_hydrogenase_gsu.
    IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    [Graphical view ]
    Pfami PF13187. Fer4_9. 1 hit.
    PF01058. Oxidored_q6. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR03294. FrhG. 1 hit.
    PROSITEi PS00198. 4FE4S_FER_1. 2 hits.
    PS51379. 4FE4S_FER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two F420-reducing hydrogenases in Methanosarcina barkeri."
      Vaupel M., Thauer R.K.
      Arch. Microbiol. 169:201-205(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes."
      Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.
      J. Bacteriol. 188:7922-7931(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Fusaro / DSM 804.
    3. "Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro."
      Michel R., Massanz C., Kostka S., Richter M., Fiebig K.
      Eur. J. Biochem. 233:727-735(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-20.

    Entry informationi

    Entry nameiFRHG_METBF
    AccessioniPrimary (citable) accession number: P80491
    Secondary accession number(s): O33167, Q46FB0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 88 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3