P80490 (FRHB_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coenzyme F420 hydrogenase subunit beta
EC=1.12.98.1

Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta
Short name=FRH

Gene names

Name:	frhB
Ordered Locus Names:	Mbar_A0449

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.
Catalytic activity	H2 + coenzyme F420 = reduced coenzyme F420.
Cofactor	Nickel. Iron-sulfur. FAD.
Subunit structure	Pentamer of two alpha chains, two beta chains and a gamma chain.
Sequence similarities	Belongs to the frhB family.

Ontologies

Keywords
Ligand	Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Molecular function	coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro iron-sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Coenzyme F420 hydrogenase subunit beta		PRO_0000159225

Sequences

	Sequence		Length	Mass (Da)
	P80490 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: 4061CC460D39EC63	FASTA	291	32,281
	10 20 30 40 50 60 MIEDPYLGKY VTCVSARSTD KEILKKAQDG GIATALMVYA LEEGFIDGTI VAGEGDKPWQ 70 80 90 100 110 120 PKPEVAMTRE EILKARGTRY SISPQISWLK EATRSFGLDK VGVTGVCCQM QAVRKAQLYP 130 140 150 160 170 180 INMRDVPGKV AFTVGLFCME NFPYKSLQSI VEDHANQSLG SVKKMEITKG KFWVYTERGN 190 200 210 220 230 240 VATVPLKATH KYEQPGCHVC LDYVSNLADI STGSVGSPDG WSTVFIRTKV GNEIWSKAVA 250 260 270 280 290 DGMFETKPIE EVKPGLDLLR KLAKEKIEKN QKTVEERKTF GVNKGLRNPY A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two F420-reducing hydrogenases in Methanosarcina barkeri." Vaupel M., Thauer R.K. Arch. Microbiol. 169:201-205(1998) [PubMed: 9477253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.
[3]	"Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro." Michel R., Massanz C., Kostka S., Richter M., Fiebig K. Eur. J. Biochem. 233:727-735(1995) [PubMed: 8521835] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y13763 Genomic DNA. Translation: CAA74093.1. CP000099 Genomic DNA. Translation: AAZ69431.1.
PIR	S63484.
RefSeq	YP_304011.1. NC_007355.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P80490.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3624325.
GenomeReviews	Gene locus Mbar_A0449 in contig CP000099_GR.
KEGG	mba:Mbar_A0449.
NMPDR	fig|269797.3.peg.627.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04838.
HOGENOM	HBG726753.
OMA	AVREYGC.
ProtClustDB	PRK09325.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0449-MONOMER. MetaCyc:MONOMER-12648.
BRENDA	1.12.98.1. 3250.
Family and domain databases
InterPro	IPR007516. Co_F420_Hydgase/DH_bsu_N. IPR017679. Coenz_F420_hydrogenase_bsu. IPR007525. FrhB_FdhB_C. [Graphical view]
KO	K00441.
Pfam	PF04432. FrhB_FdhB_C. 1 hit. PF04422. FrhB_FdhB_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR03289. FrhB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FRHB_METBF

Accession

Primary (citable) accession number: P80490
Secondary accession number(s): O33168, Q46FB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 10, 2006
Last modified:	November 16, 2011
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program