P80489 (FRHA_METBF) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 77.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Coenzyme F420 hydrogenase subunit alpha EC=1.12.98.1 Alternative name(s): 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha Short name=FRH | ||||
| Gene names |
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| Organism | Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 269797 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina |
Protein attributes
| Sequence length | 456 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen. |
| Catalytic activity | H2 + coenzyme F420 = reduced coenzyme F420. |
| Cofactor | Nickel. Iron-sulfur. FAD. |
| Subunit structure | Pentamer of two alpha chains, two beta chains and a gamma chain. |
| Subcellular location | |
| Sequence similarities | Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Ligand | FAD Metal-binding Nickel |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Cellular component | plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC ferredoxin hydrogenase activityInferred from electronic annotation. Source: InterPro flavin adenine dinucleotide bindingInferred from electronic annotation. Source: InterPro iron-sulfur cluster bindingInferred from electronic annotation. Source: InterPro nickel cation bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.3 | ||||||
| Chain | 2 – 456 | 455 | Coenzyme F420 hydrogenase subunit alpha | PRO_0000199721 | |||||
Sites | |||||||||
| Metal binding | 63 | 1 | Nickel Potential | ||||||
| Metal binding | 66 | 1 | Nickel Potential | ||||||
| Metal binding | 432 | 1 | Nickel Potential | ||||||
| Metal binding | 435 | 1 | Nickel Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Two F420-reducing hydrogenases in Methanosarcina barkeri." Vaupel M., Thauer R.K. Arch. Microbiol. 169:201-205(1998) [PubMed: 9477253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804. |
| [3] | "Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro." Michel R., Massanz C., Kostka S., Richter M., Fiebig K. Eur. J. Biochem. 233:727-735(1995) [PubMed: 8521835] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Y13763 Genomic DNA. Translation: CAA74090.1. CP000099 Genomic DNA. Translation: AAZ69434.1. |
| PIR | S63483. |
| RefSeq | YP_304014.1. NC_007355.1. |
3D structure databases | |
| ProteinModelPortal | P80489. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P80489. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 3624328. |
| GenomeReviews | Gene locus Mbar_A0452 in contig CP000099_GR. |
| KEGG | mba:Mbar_A0452. |
| NMPDR | fig|269797.3.peg.630. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | arNOG04841. |
| HOGENOM | HBG287963. |
| OMA | AREMEYT. |
| PhylomeDB | P80489. |
| ProtClustDB | CLSK876673. |
Enzyme and pathway databases | |
| BioCyc | MBAR269797:MBAR_A0452-MONOMER. MetaCyc:MONOMER-12647. |
| BRENDA | 1.12.98.1. 3250. |
Family and domain databases | |
| InterPro | IPR017682. Coenz_F420_hydrogenase_asu. IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view] |
| KO | K00440. |
| Pfam | PF00374. NiFeSe_Hases. 2 hits. [Graphical view] |
| TIGRFAMs | TIGR03295. FrhA. 1 hit. |
| PROSITE | PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | FRHA_METBF | ||||||||
| Accession | Primary (citable) accession number: P80489 Secondary accession number(s): O33165, Q46FA8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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