P80489 (FRHA_METBF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coenzyme F420 hydrogenase subunit alpha
EC=1.12.98.1

Alternative name(s):
8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha
Short name=FRH

Gene names

Name:	frhA
Ordered Locus Names:	Mbar_A0452

Organism

Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP]

Taxonomic identifier

269797 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.
Catalytic activity	H2 + coenzyme F420 = reduced coenzyme F420.
Cofactor	Nickel. Iron-sulfur. FAD.
Subunit structure	Pentamer of two alpha chains, two beta chains and a gamma chain.
Subcellular location	Cell membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	FAD Metal-binding Nickel
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coenzyme F420 hydrogenase activity Inferred from electronic annotation. Source: EC ferredoxin hydrogenase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro iron-sulfur cluster binding Inferred from electronic annotation. Source: InterPro nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3
	Chain	2 – 456	455	Coenzyme F420 hydrogenase subunit alpha		PRO_0000199721
Sites
	Metal binding	63	1	Nickel Potential
	Metal binding	66	1	Nickel Potential
	Metal binding	432	1	Nickel Potential
	Metal binding	435	1	Nickel Potential

Sequences

	Sequence		Length	Mass (Da)
	P80489 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: EEFBD0E04511D667	FASTA	456	50,769
	10 20 30 40 50 60 MTKVVEISPT TRLEGHSKLT LKVDDQGIVE RGDWLSITPV RGIEKLAIGK TMEQVPKIAS 70 80 90 100 110 120 RVCGICPIAH TLASTEAMEA SIGCEIPTDA KLLRTILHAA NRIHSIALHN ILILPDFYIP 130 140 150 160 170 180 GTEKKFNLFA NEQPARSVMA RIVRIREIAQ TIGAIAGGEA IHPSNPRIGG MYYNVSPRAK 190 200 210 220 230 240 QKMADLAKEG LVLVHEQMEF MFDVIRNMQN REFVEVAGKQ IPLPKKLGYH NQGVMATASM 250 260 270 280 290 300 YGSSSLDDNP TWDFTRWKET RPWDWYMGEV TIDLEDPSYP IGGTTKIGTK ANPQMEACTG 310 320 330 340 350 360 VPTYDGQPVE VGPRARLATF KNFDEKGTFA QHIARQMEYP DCCYTILRCL DNLNTSGKVL 370 380 390 400 410 420 ADHIPQGDGS MGWAANEAPR GSNIHLARVK DGKVLWYDML VPTTWNFPTC SRALTGAPWQ 430 440 450 IAEMVVRAYD PCVSCATHMI VVNEEEKIVT QKLMQW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two F420-reducing hydrogenases in Methanosarcina barkeri." Vaupel M., Thauer R.K. Arch. Microbiol. 169:201-205(1998) [PubMed: 9477253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Fusaro / DSM 804.
[3]	"Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro." Michel R., Massanz C., Kostka S., Richter M., Fiebig K. Eur. J. Biochem. 233:727-735(1995) [PubMed: 8521835] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y13763 Genomic DNA. Translation: CAA74090.1. CP000099 Genomic DNA. Translation: AAZ69434.1.
PIR	S63483.
RefSeq	YP_304014.1. NC_007355.1.
3D structure databases
ProteinModelPortal	P80489.
ModBase	Search...
Protein-protein interaction databases
STRING	P80489.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3624328.
GenomeReviews	Gene locus Mbar_A0452 in contig CP000099_GR.
KEGG	mba:Mbar_A0452.
NMPDR	fig|269797.3.peg.630.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04841.
HOGENOM	HBG287963.
OMA	AREMEYT.
PhylomeDB	P80489.
ProtClustDB	CLSK876673.
Enzyme and pathway databases
BioCyc	MBAR269797:MBAR_A0452-MONOMER. MetaCyc:MONOMER-12647.
BRENDA	1.12.98.1. 3250.
Family and domain databases
InterPro	IPR017682. Coenz_F420_hydrogenase_asu. IPR001501. Ni-dep_hyd_lsu. IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
KO	K00440.
Pfam	PF00374. NiFeSe_Hases. 2 hits. [Graphical view]
TIGRFAMs	TIGR03295. FrhA. 1 hit.
PROSITE	PS00507. NI_HGENASE_L_1. 1 hit. PS00508. NI_HGENASE_L_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRHA_METBF

Accession

Primary (citable) accession number: P80489
Secondary accession number(s): O33165, Q46FA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program