Reviewed, UniProtKB/Swiss-Prot P80480 (DHSB_RECAM)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] iron-sulfur subunit
    EC=1.3.5.1
Alternative name(s):
    Iron-sulfur subunit of complex II
      Short name=Ip

Gene names

Name:	SDH2
Synonyms:	SDHB

Encoded on

Mitochondrion

Organism

Reclinomonas americana

Taxonomic identifier

48483 [NCBI]

Taxonomic lineage

Eukaryota › Jakobida › Histionidae › Reclinomonas

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: a flavoprotein (FP), an iron-sulfur protein (IP), and a cytochrome b composed of a large and a small subunit By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Succinate dehydrogenase [ubiquinone] iron-sulfur subunit		PRO_0000158697
Regions
	Domain	11 – 100	90	2Fe-2S ferredoxin-type
	Domain	141 – 171	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	61	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	66	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	69	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	81	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	151	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	154	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	157	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	161	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	208	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	214	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	218	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Binding site	166	1	Ubiquinone; shared with DHSD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80480-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 781C6FF0ED55375B	FASTA	239	27,828
	10 20 30 40 50 60 MNTKKEKIML FKVYRWNPDK KEKPHISTYS VDLNSCGPMV LDALIKIKNE QDSTLTFRRS 70 80 90 100 110 120 CREGVCGSCA MNIDGTNTLA CIKSIDTNKK EMKIYPLPHM HIIKDLVPDL SNFYAQYKSI 130 140 150 160 170 180 EPWMKTTEKK LDKEFYQSRN DREKLDGLYE CVLCACCSTS CPSYWWNSDK YLGPAVLLQA 190 200 210 220 230 YRWIVDSRDQ GTRERLQYLE DPFKLYRCHT ILNCTKTCPK HLNPAQAIAK IKQNITLLT

References

[1]

"Genes encoding the same three subunits of respiratory complex II are present in the mitochondrial DNA of two phylogenetically distant eukaryotes." Burger G., Lang B.F., Reith M., Gray M.W. Proc. Natl. Acad. Sci. U.S.A. 93:2328-2332(1996) [PubMed: 8637872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 50394.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF007261 Genomic DNA. Translation: AAD11913.1.
PIR	S78180.
RefSeq	NP_044798.1.
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P07014.
ModBase	Search...
Genome annotation databases
GeneID	801067.
Enzyme and pathway databases
BRENDA	1.3.5.1. 307247.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DH/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSB_RECAM

Accession

Primary (citable) accession number: P80480

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)