P80476 (HCY6_ANDAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hemocyanin AA6 chain
Organism	Androctonus australis (Sahara scorpion)
Taxonomic identifier	6858 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Androctonus

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hemocyanins are copper-containing oxygen carriers occurring freely dissolved in the hemolymph of many mollusks and arthropods.
Subunit structure	Scorpion hemocyanin is a 24-chain polymer with 8 different chains identified, assembled in hexameric substructures.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Hemolymph.
Post-translational modification	Three disulfide bonds are present.
Miscellaneous	The two copper ions bound each have 3 nitrogen ligands (presumably contributed by His residues) and share a bridging ligand (possibly contributed by a Tyr residue) in addition to binding oxygen.
Sequence similarities	Belongs to the tyrosinase family. Hemocyanin subfamily.
Mass spectrometry	Molecular mass is 71890±7 Da from positions 1 - 626. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Oxygen transport Transport
Cellular component	Secreted
Ligand	Copper Metal-binding
PTM	Disulfide bond Phosphoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	oxidation-reduction process Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	chloride ion binding Inferred from sequence or structural similarity. Source: UniProtKB copper ion binding Inferred from sequence or structural similarity. Source: UniProtKB oxidoreductase activity Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 626	626	Hemocyanin AA6 chain		PRO_0000204253
Sites
	Metal binding	170	1	Copper 1 Probable
	Metal binding	174	1	Copper 1 Probable
	Metal binding	201	1	Copper 1 Probable
	Metal binding	321	1	Copper 2 Probable
	Metal binding	325	1	Copper 2 Probable
	Metal binding	361	1	Copper 2 Probable
Amino acid modifications
	Modified residue	374	1	Phosphoserine Probable

Sequences

	Sequence		Length	Mass (Da)
	P80476 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E788136AE3DEF0D2	FASTA	626	71,786
	10 20 30 40 50 60 TVADKQARLM PLFKHLTALT REKLPLDQRD ERLKGVGILP RGTLFSCFHA RHLAEATELY 70 80 90 100 110 120 VALYGAKDFN DFIHLCEQAR QIVNEGMFVY AVSVAVLHRE DCKGITVPPI QEVFPDRFVP 130 140 150 160 170 180 AETINRANKE ASNHPDQQSI VVEAEETGNI LDPEYKLSYF REDIGINAHH WHWHIVYPAT 190 200 210 220 230 240 WNPTVMGKEK DRKGELFFYM HQQMCARYDS ERLSNGLQRM IPFHNFDEPL EGYAPHLTSL 250 260 270 280 290 300 VSGLQYASRP EGYSIHDLSD VDVQDMVRWR ERILDAINMH YIVDKDNNKI PLDIEHGTDI 310 320 330 340 350 360 LGDIIESSDE SKNVEYYGSL HNWGHVMMAN ITDPDHRFQE NPGVMSDTST SLRDPIFYRW 370 380 390 400 410 420 HRFIDNIFQE HKKSFHPYTK EELSFPGVEV VGVSINSKTA NVITTLIKES LLELSHGINF 430 440 450 460 470 480 GTDQSVKVKY HHLDHEPFTY NIVVENNSGA EKHSTVRIFL APKYDELNNK LEPDEQRRLF 490 500 510 520 530 540 IELDKFFYTL TPGKNTIVRN HQDSSVTISK VRTFDQLGAG EGVSEDSTEY CSCGWPEHML 550 560 570 580 590 600 IPRGSHKGME FELFVMLTDH DEDTVAGLSE NAVCSDAVSY CGARDDRYPD KKAMGFPFDR 610 620 KIEARTAAEF LTPNMGLTDI KIKFHG

References

[1]	"Complete amino acid sequence of the Aa6 subunit of the scorpion Androctonus australis hemocyanin determined by Edman degradation and mass spectrometry." Buzy A., Gagnon J., Lamy J., Thibault P., Forest E., Hudry-Clergeon G. Eur. J. Biochem. 233:93-101(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Strain: Hector. Tissue: Hemolymph.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

S67964.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IXV	electron microscopy	6.80	A/C/D/E/F/G/H/I/J/K/L/M	1-626	[»]
3IXW	electron microscopy	8.00	A/C/D/E/F/G/H/I/J/K/L/M	1-626	[»]

ProteinModelPortal

P80476.

SMR

P80476. Positions 1-625.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.1280.10. 1 hit.
1.20.1370.10. 1 hit.
2.60.40.1520. 1 hit.

InterPro

IPR013788. Hemocyanin/hexamerin.
IPR000896. Hemocyanin/hexamerin_mid_dom.
IPR005203. Hemocyanin_C.
IPR005204. Hemocyanin_N.
IPR014756. Ig_E-set.
IPR002227. Tyrosinase.
IPR008922. Unchr_di-copper_centre.
[Graphical view]

PANTHER

PTHR11511. PTHR11511. 1 hit.

Pfam

PF03723. Hemocyanin_C. 1 hit.
PF00372. Hemocyanin_M. 1 hit.
PF03722. Hemocyanin_N. 1 hit.
[Graphical view]

PRINTS

PR00187. HAEMOCYANIN.

SUPFAM

SSF48056. Di-copper_centre. 1 hit.
SSF48050. Hemocyanin_N. 1 hit.
SSF81296. Ig_E-set. 1 hit.

PROSITE

PS00209. HEMOCYANIN_1. 1 hit.
PS00210. HEMOCYANIN_2. 1 hit.
PS00498. TYROSINASE_2. 1 hit.
[Graphical view]

ProtoNet

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Other

EvolutionaryTrace

P80476.

Entry information

Entry name

HCY6_ANDAU

Accession

Primary (citable) accession number: P80476

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)