Reviewed, UniProtKB/Swiss-Prot P80461 (GSHRP_TOBAC)

Names and origin

Protein names

Recommended name:
    Glutathione reductase, chloroplastic
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:

GOR

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	557 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the chloroplast.
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subcellular location	Plastid › chloroplast.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Redox-active center Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro glutathione metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 66	›66	Chloroplast Ref.2
	Chain	67 – 557	491	Glutathione reductase, chloroplastic		PRO_0000030284
Regions
	Nucleotide binding	118 – 127	10	FAD By similarity
Sites
	Active site	521	1	Proton acceptor By similarity
	Binding site	283	1	NADP By similarity
	Binding site	289	1	NADP By similarity
Amino acid modifications
	Disulfide bond	127 ↔ 132		Redox-active By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P80461-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 79813D1C6AA1D784	FASTA	557	60,035
	10 20 30 40 50 60 ATSLSTPKLS TTLSSPTLHS LLYKHKFSLL SLSNPIKPLH FNFLTHSRST PSSLSCTRRR 70 80 90 100 110 120 FTAPRAESSN GADAPRHYDF DLFTIGAGSG GVRASRFASN FGASVAVCEL PFSTISSDST 130 140 150 160 170 180 GGVGGTCVLR GCVPKKLLVY ASKYSHEFEE SCGFGWNYDV EPRFDWSTLI ANKNAELQRL 190 200 210 220 230 240 TGIYKNILKN AGVTLIEGRG KVVDPHTVDV DGKLYSAKNI LISVGGRPFI PDIPGSEYAI 250 260 270 280 290 300 DSDAALDLPT KPNKIAIVGG GYIALEFAGI FNGLKSEVHV FIRQKKVLRG FDEEIRDFVG 310 320 330 340 350 360 EQMSLRGIEF HTEESPQAIV KSADGSLSLK TSRGTVEGFS HIMFATGRRP NTKNLGLETV 370 380 390 400 410 420 GVKMTKNGAI EVDEYSRTSV PSIWAVGDVT DRINLTPVAL MEGGALAKTI FAHEPTKPDY 430 440 450 460 470 480 RNVPAAVFSQ PPIGQVGLME EQAIKEFGDV DVYTANFRPL KATISGLPDR VFMKLIVCAK 490 500 510 520 530 540 TSKVLGLHMC GDDAPEIVQG FAIAVKAGLT KADFDATVGI HPTSAEEFVT MRTPTRKVRS 550 SPSEGKAEHD IKAAAGV

References

[1]	"Cloning and characterisation of glutathione reductase cDNAs and identification of two genes encoding the tobacco enzyme." Creissen G.P., Mullineaux P.M. Planta 197:422-425(1995) [PubMed: 8547821] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Willows R.D., Kannangara G.C., Svendsen I. Submitted (JUN-1995) to UniProtKB Cited for: PROTEIN SEQUENCE OF 67-74. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X76293 mRNA. Translation: CAA53925.1.
PIR	S38908.
3D structure databases
HSSP	HSSP built from PDB template 1FEB based on UniProtKB P39040.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.8.1.7. 298.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR006324. Glut_reduct_pln. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01424. gluta_reduc_2. 1 hit.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHRP_TOBAC

Accession

Primary (citable) accession number: P80461

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)