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P80461 (GSHRP_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione reductase, chloroplastic

Short name=GR
Short name=GRase
EC=1.8.1.7
Gene names
Name:GOR
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length557 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Maintains high levels of reduced glutathione in the chloroplast.

Catalytic activity

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactor

Binds 1 FAD per subunit By similarity.

Subcellular location

Plastidchloroplast.

Miscellaneous

The active site is a redox-active disulfide bond.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide‹1 – 66›66Chloroplast Ref.2
Chain67 – 557491Glutathione reductase, chloroplastic
PRO_0000030284

Regions

Nucleotide binding118 – 12710FAD By similarity

Sites

Active site5211Proton acceptor By similarity
Binding site2831NADP By similarity
Binding site2891NADP By similarity

Amino acid modifications

Disulfide bond127 ↔ 132Redox-active By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P80461 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 79813D1C6AA1D784

FASTA55760,035
        10         20         30         40         50         60 
ATSLSTPKLS TTLSSPTLHS LLYKHKFSLL SLSNPIKPLH FNFLTHSRST PSSLSCTRRR 

        70         80         90        100        110        120 
FTAPRAESSN GADAPRHYDF DLFTIGAGSG GVRASRFASN FGASVAVCEL PFSTISSDST 

       130        140        150        160        170        180 
GGVGGTCVLR GCVPKKLLVY ASKYSHEFEE SCGFGWNYDV EPRFDWSTLI ANKNAELQRL 

       190        200        210        220        230        240 
TGIYKNILKN AGVTLIEGRG KVVDPHTVDV DGKLYSAKNI LISVGGRPFI PDIPGSEYAI 

       250        260        270        280        290        300 
DSDAALDLPT KPNKIAIVGG GYIALEFAGI FNGLKSEVHV FIRQKKVLRG FDEEIRDFVG 

       310        320        330        340        350        360 
EQMSLRGIEF HTEESPQAIV KSADGSLSLK TSRGTVEGFS HIMFATGRRP NTKNLGLETV 

       370        380        390        400        410        420 
GVKMTKNGAI EVDEYSRTSV PSIWAVGDVT DRINLTPVAL MEGGALAKTI FAHEPTKPDY 

       430        440        450        460        470        480 
RNVPAAVFSQ PPIGQVGLME EQAIKEFGDV DVYTANFRPL KATISGLPDR VFMKLIVCAK 

       490        500        510        520        530        540 
TSKVLGLHMC GDDAPEIVQG FAIAVKAGLT KADFDATVGI HPTSAEEFVT MRTPTRKVRS 

       550 
SPSEGKAEHD IKAAAGV 

« Hide

References

[1]"Cloning and characterisation of glutathione reductase cDNAs and identification of two genes encoding the tobacco enzyme."
Creissen G.P., Mullineaux P.M.
Planta 197:422-425(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Willows R.D., Kannangara G.C., Svendsen I.
Submitted (JUN-1995) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 67-74.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X76293 mRNA. Translation: CAA53925.1.
PIRS38908.

3D structure databases

ProteinModelPortalP80461.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006324. Glut-diS_reduct.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. SSF55424. 1 hit.
TIGRFAMsTIGR01424. gluta_reduc_2. 1 hit.
PROSITEPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHRP_TOBAC
AccessionPrimary (citable) accession number: P80461
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families