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Protein

Malate dehydrogenase

Gene

mdh

Organism
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NADBy similarity
Binding sitei83 – 831SubstrateBy similarity
Binding sitei89 – 891SubstrateBy similarity
Binding sitei96 – 961NADBy similarity
Binding sitei121 – 1211SubstrateBy similarity
Binding sitei152 – 1521SubstrateBy similarity
Active sitei176 – 1761Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 156NADBy similarity
Nucleotide bindingi119 – 1213NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:RPA0192
OrganismiRhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Taxonomic identifieri258594 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeRhodopseudomonas
ProteomesiUP000001426 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Malate dehydrogenasePRO_0000113465Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi258594.RPA0192.

Structurei

3D structure databases

ProteinModelPortaliP80458.
SMRiP80458. Positions 1-313.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiQKVVGMA.
OrthoDBiEOG6091FG.
PhylomeDBiP80458.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

P80458-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARDKIALIG SGQIGGTLAH LVGLKELGDV VLFDIAEGVP QGKALDIAES
60 70 80 90 100
SPVDGFDSKL TGANSYEAIE GARVVIVTAG VPRKPGMSRD DLLSINLKVM
110 120 130 140 150
EQVGAGIKKY APDAFVICIT NPLDAMVWAL QKASGLPAKK VVGMAGVLDS
160 170 180 190 200
ARFRYFLADE FNVSVEDVTA FVLGGHGDTM VPLVKYSTVA GIPLPDLVKM
210 220 230 240 250
GWTSQARLDE IVDRTRNGGA EIVNLLKTGS AFYAPASSAI AMAESYLKDK
260 270 280 290 300
KRVVPVAAHL NGEYGVKDMY VGVPVVIGDK GVERIVEIEL AGKDKEAFDR
310 320
SVAAVQGLVE ACKKIAPDLL GR
Length:322
Mass (Da):33,996
Last modified:February 15, 2004 - v2
Checksum:i8F41556B89B2D187
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 131Q → E AA sequence (Ref. 2) Curated
Sequence conflicti21 – 222LV → MI AA sequence (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572593 Genomic DNA. Translation: CAE25636.1.
RefSeqiNP_945545.1. NC_005296.1.

Genome annotation databases

KEGGirpa:RPA0192.
PATRICi23284495. VBIRhoPal84835_0199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX572593 Genomic DNA. Translation: CAE25636.1.
RefSeqiNP_945545.1. NC_005296.1.

3D structure databases

ProteinModelPortaliP80458.
SMRiP80458. Positions 1-313.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi258594.RPA0192.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGirpa:RPA0192.
PATRICi23284495. VBIRhoPal84835_0199.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiQKVVGMA.
OrthoDBiEOG6091FG.
PhylomeDBiP80458.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-98 / CGA009.
  2. Naterstad K., Synstad B., Sirevag R.
    Submitted (AUG-1996) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 5-25.

Entry informationi

Entry nameiMDH_RHOPA
AccessioniPrimary (citable) accession number: P80458
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 14, 1998
Last sequence update: February 15, 2004
Last modified: March 31, 2015
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.