Reviewed, UniProtKB/Swiss-Prot P80447 (G3P2_JACOR)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, liver
      Short name=GAPDH
    EC=1.2.1.12

Gene names

Name:	GAPDH
Synonyms:	GAPC, GAPD

Organism

Jaculus orientalis (Greater Egyptian jerboa)

Taxonomic identifier

48868 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Dipodidae › Dipodinae › Jaculus

Protein attributes

Sequence length	34 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Interacts with EIF1AD By similarity. Homotetramer.
Subcellular location	Cytoplasm.
Post-translational modification	The hibernating adult isoform is ADP-ribosylated. This accounts for 2-3 fold lower specific activity in the hibernating isoform.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	ADP-ribosylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›34	›34	Glyceraldehyde-3-phosphate dehydrogenase, liver		PRO_0000145488
Regions
	Nucleotide binding	10 – 11	2	NAD By similarity
Sites
	Binding site	32	1	NAD By similarity
Natural variations
	Natural variant	6	1	N → D in hibernating adult liver isoform.
Experimental info
	Non-terminal residue	34	1

Sequences

	Sequence		Length	Mass (Da)
	P80447-1 [UniParc]. Last modified July 5, 2004. Version 3. Checksum: F53F42C52A7C041A	FASTA	34	3,588
	10 20 30 VKVGVNGFGR IGRLVTRAAF NSGKVDIVAI NDPF

References

[1]	"Occurrence of a differential expression of the glyceraldehyde-3-phosphate dehydrogenase gene in muscle and liver from euthermic and induced hibernating jerboa (Jaculus orientalis)." Soukri A., Valverde F., Hafid N., Elkebbaj M.S., Serrano A. Gene 181:139-145(1996) [PubMed: 8973322] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"Evidence for a posttranslational covalent modification of liver glyceraldehyde-3-phosphate dehydrogenase in hibernating jerboa (Jaculus orientalis)." Soukri A., Hafid N., Valverde F., Elkebbaj M.S., Serrano A. Biochim. Biophys. Acta 1292:177-187(1996) [PubMed: 8547342] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-24. Tissue: Liver.

Cross-references

3D structure databases
SMR	P80447. Positions 1-34.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P80447.
Enzyme and pathway databases
BRENDA	1.2.1.12. 269396.
Family and domain databases
InterPro	IPR020828. GlycerAld_3-P_DH_NAD(P)_bd. IPR000173. GlycerAld_3-P_DH_subfam. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF00044. Gp_dh_N. 1 hit. [Graphical view]
PROSITE	PS00071. GAPDH. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P2_JACOR

Accession

Primary (citable) accession number: P80447

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)