Actin-related protein 4 - Saccharomyces cerevisiae (Baker's yeast)

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Reviewed, UniProtKB/Swiss-Prot P80428 (ARP4_YEAST)

Last modified June 16, 2009. Version 78. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Actin-related protein 4
Alternative name(s):
    Actin-like protein ARP4
      Short name=Actin-like protein 4

Gene names

Name:	ARP4
Synonyms:	ACT3
Ordered Locus Names:	YJL081C
ORF Names:	J1012

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Chromatin interaction component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. ARP4 recognizes H2AS128ph (gamma-H2A) and is required for NuA4 complex integrity. Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the INO80 complex which remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to gamma-H2A as a response to DNA damage. Ref.5 Ref.7 Ref.8 Ref.10 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17
Subunit structure	Component of the NuA4 histone acetyltransferase complex composed of at least ACT1, ARP4, EAF3, EAF5, EAF6, EAF7, EPL1, ESA1, SWC4, TRA1, VID21, YAF9 and YNG2. Component of the chromatin-remodeling INO80 complex, at least composed of ARP4, ARP5, ARP8, RVB1, RVB2, TAF14, NHP10, IES1, IES3, IES4, IES6, ACT1, IES2, IES5 and INO80. Component of the SWR1 chromatin remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Interacts with histones H4 (HHF1 and HHF2), H3 (HHT1 and HHT2) and H2A (HTA1 and HTA2). Ref.5
Subcellular location	Nucleus. Ref.6
Miscellaneous	Present with 1070 molecules/cell in log phase SD medium. Ref.12
Sequence similarities	Belongs to the actin family. ARP4 subfamily.

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Ontologies

Keywords
Biological process	DNA damage DNA repair Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Molecular function	Activator Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA repair Ref.10 Ref.14 Inferred from direct assay. Source: SGD chromatin remodeling Ref.17 Inferred from direct assay. Source: SGD histone acetylation Ref.5 Inferred from direct assay. Source: SGD kinetochore assembly Inferred from mutant phenotype. Source: SGD regulation of transcription from RNA polymerase II promoter Ref.5 Inferred from direct assay. Source: SGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Ino80 complex Ref.7 Inferred from physical interaction. Source: SGD NuA4 histone acetyltransferase complex Ref.5 Inferred from physical interaction. Source: SGD Swr1 complex Ref.13 Ref.17 Inferred from direct assay. Source: SGD
Molecular function	chromatin binding Inferred from direct assay. Source: SGD protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
ECM4	P36156	1	EBI-2939,EBI-2042717
ESA1	Q08649	1	EBI-2939,EBI-6648

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 489

489

Actin-related protein 4

PRO_0000089103

Amino acid modifications

Modified residue

338

Phosphothreonine Ref.18

Modified residue

349

Phosphoserine Ref.18

Modified residue

353

Phosphoserine Ref.18

Experimental info

Mutagenesis

S → A: Lethal; when associated with D-161. Formamide-, hydroxyurea and UV-hypersensitivity, suppressor of TY phenotype; when associated with A-159. Ref.15

Mutagenesis

155

C → Y: No histone acetyltransferase activity at 37 degrees celcius. Ref.5

Mutagenesis

159

D → A: Formamide-, hydroxyurea and UV-hypersensitivity, suppressor of TY phenotype; when associated with S-23. Ref.15

Mutagenesis

161

G → D: Formamide-, hydroxyurea and UV-hypersensitivity, destabilization of ARP4, suppressor of TY phenotype and elevated levels of MSN2/MSN4-regulated genes. Lethal; when associated with A-23. Ref.15

Mutagenesis

187

G → R: Defect in promoter association and change in chromatin structure. Ref.8

Mutagenesis

455

G → S: No histone acetyltransferase activity at 37 degrees celcius. Defect in promoter association and change in chromatin structure. Ref.5 Ref.8

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Sequences

Sequence

Length

Mass (Da)

Tools

P80428-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 6DE1C8938C6C3DBB
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FASTA

489

54,832

        10         20         30         40         50         60 
MSNAALQVYG GDEVSAVVID PGSYTTNIGY SGSDFPQSIL PSVYGKYTAD EGNKKIFSEQ 

        70         80         90        100        110        120 
SIGIPRKDYE LKPIIENGLV IDWDTAQEQW QWALQNELYL NSNSGIPALL TEPVWNSTEN 

       130        140        150        160        170        180 
RKKSLEVLLE GMQFEACYLA PTSTCVSFAA GRPNCLVVDI GHDTCSVSPI VDGMTLSKST 

       190        200        210        220        230        240 
RRNFIAGKFI NHLIKKALEP KEIIPLFAIK QRKPEFIKKT FDYEVDKSLY DYANNRGFFQ 

       250        260        270        280        290        300 
ECKETLCHIC PTKTLEETKT ELSSTAKRSI ESPWNEEIVF DNETRYGFAE ELFLPKEDDI 

       310        320        330        340        350        360 
PANWPRSNSG VVKTWRNDYV PLKRTKPSGV NKSDKKVTPT EEKEQEAVSK STSPAANSAD 

       370        380        390        400        410        420 
TPNETGKRPL EEEKPPKENN ELIGLADLVY SSIMSSDVDL RATLAHNVVL TGGTSSIPGL 

       430        440        450        460        470        480 
SDRLMTELNK ILPSLKFRIL TTGHTIERQY QSWLGGSILT SLGTFHQLWV GKKEYEEVGV 


ERLLNDRFR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An essential gene of Saccharomyces cerevisiae coding for an actin-related protein." Harata M., Karwan A., Wintersberger U. Proc. Natl. Acad. Sci. U.S.A. 91:8258-8262(1994) [PubMed: 8058791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erratum Harata M., Karwan A., Wintersberger U. Proc. Natl. Acad. Sci. U.S.A. 91:10757-10757(1994)
[3]	"Sequence analysis of a 33.1 kb fragment from the left arm of Saccharomyces cerevisiae chromosome X, including putative proteins with leucine zippers, a fungal Zn(II)2-Cys6 binuclear cluster domain and a putative alpha 2-SCB-alpha 2 binding site." Miosga T., Schaaff-Gerstenschlaeger I., Chalwatzis N., Baur A., Boles E., Fournier C., Schmitt S., Velten C., Wilhelm N., Zimmermann F.K. Yeast 11:681-689(1995) [PubMed: 7483841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	"Multiple links between the NuA4 histone acetyltransferase complex and epigenetic control of transcription." Galarneau L., Nourani A., Boudreault A.A., Zhang Y., Heliot L., Allard S., Savard J., Lane W.S., Stillman D.J., Cote J. Mol. Cell 5:927-937(2000) [PubMed: 10911987] [Abstract] Cited for: PROTEIN SEQUENCE OF 55-66; 202-210; 220-243; 269-296; 317-323; 378-430 AND 448-482, IDENTIFICATION IN THE NUA4 COMPLEX, INTERACTION WITH HISTONES H2A; H3 AND H4, MUTAGENESIS OF CYS-155 AND GLY-455, FUNCTION.
[6]	"The actin-related protein Act3p of Saccharomyces cerevisiae is located in the nucleus." Weber V., Harata M., Hauser H., Wintersberger U. Mol. Biol. Cell 6:1263-1270(1995) [PubMed: 8573785] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"A chromatin remodelling complex involved in transcription and DNA processing." Shen X., Mizuguchi G., Hamiche A., Wu C. Nature 406:541-544(2000) [PubMed: 10952318] [Abstract] Cited for: IDENTIFICATION IN THE INO80 COMPLEX, FUNCTION OF THE INO80 COMPLEX, MASS SPECTROMETRY.
[8]	"Correlation between chromatin association and transcriptional regulation for the Act3p/Arp4 nuclear actin-related protein of Saccharomyces cerevisiae." Harata M., Zhang Y., Stillman D.J., Matsui D., Oma Y., Nishimori K., Mochizuki R. Nucleic Acids Res. 30:1743-1750(2002) [PubMed: 11937627] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF GLY-187 AND GLY-455.
[9]	"Who's who among the Saccharomyces cerevisiae actin-related proteins? A classification and nomenclature proposal for a large family." Poch O., Winsor B. Yeast 13:1053-1058(1997) [PubMed: 9290209] [Abstract] Cited for: NOMENCLATURE.
[10]	"Acetylation of histone H4 by Esa1 is required for DNA double-strand break repair." Bird A.W., Yu D.Y., Pray-Grant M.G., Qiu Q., Harmon K.E., Megee P.C., Grant P.A., Smith M.M., Christman M.F. Nature 419:411-415(2002) [PubMed: 12353039] [Abstract] Cited for: FUNCTION.
[11]	"Involvement of actin-related proteins in ATP-dependent chromatin remodeling." Shen X., Ranallo R., Choi E., Wu C. Mol. Cell 12:147-155(2003) [PubMed: 12887900] [Abstract] Cited for: IDENTIFICATION IN THE INO80 COMPLEX, MASS SPECTROMETRY.
[12]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[13]	"A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1." Krogan N.J., Keogh M.-C., Datta N., Sawa C., Ryan O.W., Ding H., Haw R.A., Pootoolal J., Tong A., Canadien V., Richards D.P., Wu X., Emili A., Hughes T.R., Buratowski S., Greenblatt J.F. Mol. Cell 12:1565-1576(2003) [PubMed: 14690608] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE SWR1 COMPLEX, MASS SPECTROMETRY.
[14]	"Binding of chromatin-modifying activities to phosphorylated histone H2A at DNA damage sites." Downs J.A., Allard S., Jobin-Robitaille O., Javaheri A., Auger A., Bouchard N., Kron S.J., Jackson S.P., Cote J. Mol. Cell 16:979-990(2004) [PubMed: 15610740] [Abstract] Cited for: FUNCTION.
[15]	"The nuclear actin-related protein Act3p/Arp4p of Saccharomyces cerevisiae is involved in transcription regulation of stress genes." Goerzer I., Schueller C., Heidenreich E., Krupanska L., Kuchler K., Wintersberger U. Mol. Microbiol. 50:1155-1171(2003) [PubMed: 14622406] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF SER-23; ASP-159 AND GLY-161.
[16]	"A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin." Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J. PLoS Biol. 2:587-599(2004) [PubMed: 15045029] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE SWR1 COMPLEX, MASS SPECTROMETRY.
[17]	"ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex." Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C. Science 303:343-348(2004) [PubMed: 14645854] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[18]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-338; SER-349 AND SER-353, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X75317 Genomic DNA. Translation: CAA53066.1. X83502 Genomic DNA. Translation: CAA58489.1. Z49356 Genomic DNA. Translation: CAA89374.1.
PIR	S47608.
RefSeq	NP_012454.1.
3D structure databases
HSSP	HSSP built from PDB template 1NMD based on UniProtKB P02577.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2361N.
IntAct	P80428. 37 interactions.
Proteomic databases
PeptideAtlas	P80428.
Genome annotation databases
Ensembl	YJL081C. Saccharomyces cerevisiae. [Contig view]
GeneID	853364.
GenomeReviews	Gene locus YJL081C in contig Y13136_GR.
KEGG	sce:YJL081C.
NMPDR	fig\|4932.3.peg.3423.
Organism-specific databases
CYGD	YJL081c.
SGD	S000003617. ARP4.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P80428.
OMA	P80428. GTFHQMW.
Gene expression databases
GermOnline	YJL081C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004000. Actin-like. IPR004001. Actin_CS. [Graphical view]
PANTHER	PTHR11937. Actin_like. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS00406. ACTINS_1. False negative. PS00432. ACTINS_2. False negative. PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973792.

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Entry information

Entry name

ARP4_YEAST

Accession

Primary (citable) accession number: P80428

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents