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Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

MTHFS

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-CHO-H4PteGlu) to yield 5,10-methenyltetrahydrofolate. It can also use 5-formyltetrahydropteroic acid (5-CHO-H4Pte) and 5-formyltetrahydropteroylhistidine (5-CHO-H4PteHis) as substrate.1 Publication

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.

Cofactori

Mg2+By similarity

Kineticsi

Kcat is 2 sec(-1) for cyclo-ligase activity with 5-CHO-H4Pte. Kcat is 3 sec(-1) for cyclo-ligase activity with 5-CHO-H4PteHis. Kcat is 5 sec(-1) for cyclo-ligase activity with 5-CHO-H4PteGlu and 5-CHO-H4PteGlu5.
  1. KM=0.2 µM for 5-CHO-H4PteGlu51 Publication
  2. KM=8 µM for 5-CHO-H4PteGlu1 Publication
  3. KM=11 µM for 5-CHO-H4PteHis1 Publication
  4. KM=21 µM for 5-CHO-H4Pte1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei14ATPBy similarity1
    Binding sitei56Substrate; via carbonyl oxygenBy similarity1
    Binding sitei61SubstrateBy similarity1
    Metal bindingi154MagnesiumBy similarity1
    Metal bindingi189MagnesiumBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi10 – 14ATPBy similarity5
    Nucleotide bindingi145 – 153ATPBy similarity9

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionLigase
    LigandATP-binding, Folate-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
    Alternative name(s):
    5,10-methenyl-tetrahydrofolate synthetase
    Short name:
    MTHFS
    Short name:
    Methenyl-THF synthetase
    Gene namesi
    Name:MTHFS
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    Proteomesi
    • UP000001811 Componenti: Unplaced

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002002771 – 2015-formyltetrahydrofolate cyclo-ligaseAdd BLAST201

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei1N-acetylalanine1 Publication1

    Keywords - PTMi

    Acetylation

    PTM databases

    iPTMnetiP80405

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP80405
    SMRiP80405
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni148 – 152Substrate bindingBy similarity5

    Sequence similaritiesi

    Phylogenomic databases

    HOVERGENiHBG052525
    InParanoidiP80405

    Family and domain databases

    Gene3Di3.40.50.10420, 1 hit
    InterProiView protein in InterPro
    IPR002698 FTHF_cligase
    IPR024185 FTHF_cligase-like_sf
    IPR037171 NagB/RpiA_transferase-like
    PANTHERiPTHR23407:SF1 PTHR23407:SF1, 1 hit
    PfamiView protein in Pfam
    PF01812 5-FTHF_cyc-lig, 1 hit
    PIRSFiPIRSF006806 FTHF_cligase, 1 hit
    SUPFAMiSSF100950 SSF100950, 1 hit
    TIGRFAMsiTIGR02727 MTHFS_bact, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P80405-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    AAAAAVSGAK RSLRAELKQR LRAISAEERL RCQRLLTQKV IAHRQYQKSQ
    60 70 80 90 100
    RISIFLSMPD EIETEEIIKD IFQQGKVCFI PRYRLQSNHM DMVKLASADE
    110 120 130 140 150
    ISSLPKTSWN IHQPSESDTR EEALATGGLD LIFMPGLGFD RNGNRLGRGR
    160 170 180 190 200
    GYYDTYLQRC LQQQGAKPYT IALAFREQIC PQVPVDDTDV SVDEVLYVDA

    A
    Length:201
    Mass (Da):22,733
    Last modified:November 1, 1995 - v1
    Checksum:i6FD20B8D9C31EC46
    GO

    Sequence databases

    PIRiA53688

    Similar proteinsi

    Entry informationi

    Entry nameiMTHFS_RABIT
    AccessioniPrimary (citable) accession number: P80405
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: April 25, 2018
    This is version 88 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health