Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

30S ribosomal protein S15

Gene

rpsO

Organism
Thermus thermophilus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.
Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
30S ribosomal protein S15UniRule annotation
Gene namesi
Name:rpsOUniRule annotation
Synonyms:rps15UniRule annotation
OrganismiThermus thermophilus
Taxonomic identifieri274 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 898830S ribosomal protein S15PRO_0000115572Add
BLAST

Interactioni

Subunit structurei

Part of the 30S ribosomal subunit. Forms a bridge to the 50S subunit in the 70S ribosome, contacting the 23S rRNA.UniRule annotation

Protein-protein interaction databases

DIPiDIP-6174N.

Structurei

Secondary structure

1
89
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1511Combined sources
Turni19 – 224Combined sources
Helixi26 – 4520Combined sources
Turni46 – 483Combined sources
Helixi51 – 7323Combined sources
Helixi75 – 817Combined sources
Turni82 – 865Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AB3NMR-A2-89[»]
1DK1X-ray2.80A2-87[»]
1F7YX-ray2.80A1-89[»]
1KUQX-ray2.84A1-87[»]
1TWTmodel-R2-89[»]
2FKXNMR-A2-89[»]
4V49X-ray8.70O2-89[»]
4V8XX-ray3.35AO/CO1-89[»]
ProteinModelPortaliP80378.
SMRiP80378. Positions 2-89.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80378.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S15P family.UniRule annotation

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
HAMAPiMF_01343_B. Ribosomal_S15_B.
InterProiIPR000589. Ribosomal_S15.
IPR005290. Ribosomal_S15_bac-type.
IPR009068. S15_NS1_RNA-bd.
[Graphical view]
PfamiPF00312. Ribosomal_S15. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
TIGRFAMsiTIGR00952. S15_bact. 1 hit.
PROSITEiPS00362. RIBOSOMAL_S15. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80378-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPITKEEKQK VIQEFARFPG DTGSTEVQVA LLTLRINRLS EHLKVHKKDH
60 70 80
HSHRGLLMMV GQRRRLLRYL QREDPERYRA LIEKLGIRG
Length:89
Mass (Da):10,554
Last modified:January 23, 2007 - v3
Checksum:i9DB7E1C533DBF340
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37726 Genomic DNA. Translation: CAA85782.1.
Z84207 Genomic DNA. Translation: CAB06340.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z37726 Genomic DNA. Translation: CAA85782.1.
Z84207 Genomic DNA. Translation: CAB06340.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AB3NMR-A2-89[»]
1DK1X-ray2.80A2-87[»]
1F7YX-ray2.80A1-89[»]
1KUQX-ray2.84A1-87[»]
1TWTmodel-R2-89[»]
2FKXNMR-A2-89[»]
4V49X-ray8.70O2-89[»]
4V8XX-ray3.35AO/CO1-89[»]
ProteinModelPortaliP80378.
SMRiP80378. Positions 2-89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6174N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP80378.

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
HAMAPiMF_01343_B. Ribosomal_S15_B.
InterProiIPR000589. Ribosomal_S15.
IPR005290. Ribosomal_S15_bac-type.
IPR009068. S15_NS1_RNA-bd.
[Graphical view]
PfamiPF00312. Ribosomal_S15. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
TIGRFAMsiTIGR00952. S15_bact. 1 hit.
PROSITEiPS00362. RIBOSOMAL_S15. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ribosomal protein S15 from Thermus thermophilus -- cloning, sequencing, overexpression of the gene and RNA-binding properties of the protein."
    Serganov A., Rak A., Garber M.B., Reinbolt J., Ehresmann B., Ehresmann C., Grunberg-Manago M., Portier C.
    Eur. J. Biochem. 246:291-300(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: VK1.
  2. "Polynucleotide phosphorylase from Thermus thermophilus: cloning, sequencing and expression of the gene and biochemical properties of the enzyme."
    Serganov A.A., Garber M.B., Portier C.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: VK1.
  3. "Solution structure of the ribosomal RNA binding protein S15 from Thermus thermophilus."
    Berglund H., Rak A., Serganov A., Garber M.B., Haerd T.
    Nat. Struct. Biol. 4:20-23(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  4. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    Strain: VK1.
  5. Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF S15 AND RRNA.
    Strain: VK1.

Entry informationi

Entry nameiRS15_THETH
AccessioniPrimary (citable) accession number: P80378
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: April 29, 2015
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.