P80358 (ASTG_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine N-succinyltransferase subunit beta
EC=2.3.1.109

Alternative name(s):
AOST
Short name=AST
ARUAII

Gene names

Name:	aruG
Ordered Locus Names:	PA0897

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Succinyl-CoA + L-arginine = CoA + N(2)-succinyl-L-arginine.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 1/5.
Subunit structure	Heterotetramer of two alpha and two beta subunits.
Sequence similarities	Belongs to the succinylarginine dihydrolase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	arginine catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	arginine N-succinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Arginine N-succinyltransferase subunit beta		PRO_0000064717

Sequences

	Sequence		Length	Mass (Da)
	P80358 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: B87B6626166DD49A	FASTA	340	37,245
	10 20 30 40 50 60 MIVRPVTSAD LPALIELARS TGTGLTTLPA NEQRLQHRVS WAEKAFRGEA ERGDADYLFV 70 80 90 100 110 120 LEDDAGKVVG ISAIAGAVGL REPWYNYRVG LTVSASQELN IHREIPTLFL ANDLTGNSEL 130 140 150 160 170 180 CSLFLHADHR SGLNGKLLSR ARFLFIAEFR HLFGDKLIAE MRGMSDEEGR SPFWESLGRH 190 200 210 220 230 240 FFKMEFSQAD YLTGVGNKAF IAELMPKFPL YTCFLSEEAR GVIGRVHPNT EPALAMLKAE 250 260 270 280 290 300 GFSYQGYVDI FDAGPAIEAE TDKIRAIAES QNLVLAVGTP GDDAEPYLIH NRKREDCRIT 310 320 330 340 AAPARAAAGT LVVDPLTAKR LRLSAGASVR AVPLSAQKRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa." Itoh Y. J. Bacteriol. 179:7280-7290(1997) [PubMed: 9393691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Purification and properties of a succinyltransferase from Pseudomonas aeruginosa specific for both arginine and ornithine." Tricot C., Vander Wauven C., Wattiez R., Falmagne P., Stalon V. Eur. J. Biochem. 224:853-861(1994) [PubMed: 7523119] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF011922 Genomic DNA. Translation: AAC46011.1. AE004091 Genomic DNA. Translation: AAG04286.1.
PIR	B83533.
RefSeq	NP_249588.1. NC_002516.2.
3D structure databases
ProteinModelPortal	P80358.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883082.
GenomeReviews	Gene locus PA0897 in contig AE004091_GR.
KEGG	pae:PA0897.
PATRIC	19836080. VBIPseAer58763_0932.
Organism-specific databases
PseudoCAP	PA0897.
Phylogenomic databases
HOGENOM	HBG377601.
OMA	AGPAIEC.
ProtClustDB	CLSK866200.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-11525. PAER208964:PA0897-MONOMER.
BRENDA	2.3.1.109. 5087.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR007041. Arg_N-succinylTrfase_Ast/AOST. IPR017650. Arginine_N-succinylTrfase_AstA. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
KO	K00673.
Pfam	PF04958. AstA. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR03243. Arg_catab_AOST. 1 hit. TIGR03244. Arg_catab_AstA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTG_PSEAE

Accession

Primary (citable) accession number: P80358

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 15, 1999
Last modified:	January 25, 2012
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program