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Protein

Arginine N-succinyltransferase subunit alpha

Gene

astA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of succinyl-CoA to arginine to produce N(2)-succinylarginine. Also acts on L-ornithine.

Catalytic activityi

Succinyl-CoA + L-arginine = CoA + N(2)-succinyl-L-arginine.

Pathwayi: L-arginine degradation via AST pathway

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Arginine N-succinyltransferase subunit beta (aruG), Arginine N-succinyltransferase subunit alpha (astA)
  2. N-succinylarginine dihydrolase (astB)
  3. Succinylornithine transaminase/acetylornithine aminotransferase (aruC)
  4. N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
  5. Succinylglutamate desuccinylase (astE)
This subpathway is part of the pathway L-arginine degradation via AST pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

  • arginine catabolic process Source: PseudoCAP
  • arginine catabolic process to succinate Source: UniProtKB-UniPathway

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processArginine metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11524.
UniPathwayiUPA00185; UER00279.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine N-succinyltransferase subunit alpha (EC:2.3.1.109)
Short name:
ARUAI
Alternative name(s):
AOST
Short name:
AST
Gene namesi
Name:astA
Synonyms:aruF
Ordered Locus Names:PA0896
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0896.

Pathology & Biotechi

Chemistry databases

DrugBankiDB01942. Formic Acid.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000647141 – 338Arginine N-succinyltransferase subunit alphaAdd BLAST338

Proteomic databases

PaxDbiP80357.
PRIDEiP80357.

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta subunits.

Protein-protein interaction databases

STRINGi208964.PA0896.

Structurei

Secondary structure

1338
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi9 – 11Combined sources3
Helixi12 – 21Combined sources10
Helixi33 – 48Combined sources16
Beta strandi58 – 65Combined sources8
Turni66 – 68Combined sources3
Beta strandi71 – 80Combined sources10
Beta strandi83 – 85Combined sources3
Beta strandi89 – 99Combined sources11
Turni100 – 103Combined sources4
Beta strandi104 – 114Combined sources11
Turni116 – 119Combined sources4
Beta strandi120 – 128Combined sources9
Helixi130 – 132Combined sources3
Helixi136 – 151Combined sources16
Helixi153 – 155Combined sources3
Beta strandi158 – 163Combined sources6
Helixi175 – 179Combined sources5
Helixi181 – 183Combined sources3
Helixi189 – 198Combined sources10
Helixi215 – 217Combined sources3
Helixi220 – 225Combined sources6
Helixi231 – 233Combined sources3
Helixi234 – 243Combined sources10
Beta strandi246 – 251Combined sources6
Turni253 – 255Combined sources3
Beta strandi258 – 263Combined sources6
Helixi264 – 266Combined sources3
Helixi268 – 271Combined sources4
Beta strandi274 – 280Combined sources7
Beta strandi288 – 293Combined sources6
Beta strandi302 – 307Combined sources6
Beta strandi315 – 317Combined sources3
Helixi319 – 325Combined sources7
Beta strandi332 – 337Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YLEX-ray1.70A1-338[»]
ProteinModelPortaliP80357.
SMRiP80357.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80357.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108T2Z. Bacteria.
COG3138. LUCA.
HOGENOMiHOG000258231.
KOiK00673.
OMAiETDHYID.
PhylomeDBiP80357.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR017651. Arg/Orn_succinylTfrase_asu.
IPR007041. Arg_succinylTrfase_AstA/AruG.
PfamiView protein in Pfam
PF04958. AstA. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR03245. arg_AOST_alph. 1 hit.
TIGR03243. arg_catab_AOST. 1 hit.

Sequencei

Sequence statusi: Complete.

P80357-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVMRPAQAA DLPQVQRLAA DSPVGVTSLP DDAERLRDKI LASEASFAAE
60 70 80 90 100
VSYNGEESYF FVLEDSASGE LVGCSAIVAS AGFSEPFYSF RNETFVHASR
110 120 130 140 150
SLSIHNKIHV LSLCHDLTGN SLLTSFYVQR DLVQSVYAEL NSRGRLLFMA
160 170 180 190 200
SHPERFADAV VVEIVGYSDE QGESPFWNAV GRNFFDLNYI EAEKLSGLKS
210 220 230 240 250
RTFLAELMPH YPIYVPLLPD AAQESMGQVH PRAQITFDIL MREGFETDNY
260 270 280 290 300
IDIFDGGPTL HARTSGIRSI AQSRVVPVKI GEAPKSGRPY LVTNGQLQDF
310 320 330
RAVVLDLDWA PGKPVALSVE AAEALGVGEG ASVRLVAV
Length:338
Mass (Da):36,931
Last modified:July 15, 1999 - v2
Checksum:i7152BB5EC220F719
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011922 Genomic DNA. Translation: AAC46010.1.
AE004091 Genomic DNA. Translation: AAG04285.1.
PIRiA83533.
RefSeqiNP_249587.1. NC_002516.2.
WP_003085950.1. NZ_ASJY01000130.1.

Genome annotation databases

EnsemblBacteriaiAAG04285; AAG04285; PA0896.
GeneIDi879437.
KEGGipae:PA0896.
PATRICi19836078. VBIPseAer58763_0931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011922 Genomic DNA. Translation: AAC46010.1.
AE004091 Genomic DNA. Translation: AAG04285.1.
PIRiA83533.
RefSeqiNP_249587.1. NC_002516.2.
WP_003085950.1. NZ_ASJY01000130.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YLEX-ray1.70A1-338[»]
ProteinModelPortaliP80357.
SMRiP80357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0896.

Chemistry databases

DrugBankiDB01942. Formic Acid.

Proteomic databases

PaxDbiP80357.
PRIDEiP80357.

Protocols and materials databases

DNASUi879437.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04285; AAG04285; PA0896.
GeneIDi879437.
KEGGipae:PA0896.
PATRICi19836078. VBIPseAer58763_0931.

Organism-specific databases

PseudoCAPiPA0896.

Phylogenomic databases

eggNOGiENOG4108T2Z. Bacteria.
COG3138. LUCA.
HOGENOMiHOG000258231.
KOiK00673.
OMAiETDHYID.
PhylomeDBiP80357.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00279.
BioCyciMetaCyc:MONOMER-11524.

Miscellaneous databases

EvolutionaryTraceiP80357.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR017651. Arg/Orn_succinylTfrase_asu.
IPR007041. Arg_succinylTrfase_AstA/AruG.
PfamiView protein in Pfam
PF04958. AstA. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR03245. arg_AOST_alph. 1 hit.
TIGR03243. arg_catab_AOST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASTA_PSEAE
AccessioniPrimary (citable) accession number: P80357
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1999
Last modified: March 15, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.