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Protein

Arginine N-succinyltransferase subunit alpha

Gene

astA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of succinyl-CoA to arginine to produce N(2)-succinylarginine. Also acts on L-ornithine.

Catalytic activityi

Succinyl-CoA + L-arginine = CoA + N(2)-succinyl-L-arginine.

Pathwayi: L-arginine degradation via AST pathway

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate and succinate from L-arginine.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Arginine N-succinyltransferase subunit beta (aruG), Arginine N-succinyltransferase subunit alpha (astA)
  2. N-succinylarginine dihydrolase (astB)
  3. Succinylornithine transaminase/acetylornithine aminotransferase (aruC)
  4. N-succinylglutamate 5-semialdehyde dehydrogenase (astD)
  5. Succinylglutamate desuccinylase (astE)
This subpathway is part of the pathway L-arginine degradation via AST pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate and succinate from L-arginine, the pathway L-arginine degradation via AST pathway and in Amino-acid degradation.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Arginine metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11524.
UniPathwayiUPA00185; UER00279.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine N-succinyltransferase subunit alpha (EC:2.3.1.109)
Short name:
ARUAI
Alternative name(s):
AOST
Short name:
AST
Gene namesi
Name:astA
Synonyms:aruF
Ordered Locus Names:PA0896
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0896.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Arginine N-succinyltransferase subunit alphaPRO_0000064714Add
BLAST

Proteomic databases

PaxDbiP80357.

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta subunits.

Protein-protein interaction databases

STRINGi208964.PA0896.

Structurei

Secondary structure

1
338
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi9 – 113Combined sources
Helixi12 – 2110Combined sources
Helixi33 – 4816Combined sources
Beta strandi58 – 658Combined sources
Turni66 – 683Combined sources
Beta strandi71 – 8010Combined sources
Beta strandi83 – 853Combined sources
Beta strandi89 – 9911Combined sources
Turni100 – 1034Combined sources
Beta strandi104 – 11411Combined sources
Turni116 – 1194Combined sources
Beta strandi120 – 1289Combined sources
Helixi130 – 1323Combined sources
Helixi136 – 15116Combined sources
Helixi153 – 1553Combined sources
Beta strandi158 – 1636Combined sources
Helixi175 – 1795Combined sources
Helixi181 – 1833Combined sources
Helixi189 – 19810Combined sources
Helixi215 – 2173Combined sources
Helixi220 – 2256Combined sources
Helixi231 – 2333Combined sources
Helixi234 – 24310Combined sources
Beta strandi246 – 2516Combined sources
Turni253 – 2553Combined sources
Beta strandi258 – 2636Combined sources
Helixi264 – 2663Combined sources
Helixi268 – 2714Combined sources
Beta strandi274 – 2807Combined sources
Beta strandi288 – 2936Combined sources
Beta strandi302 – 3076Combined sources
Beta strandi315 – 3173Combined sources
Helixi319 – 3257Combined sources
Beta strandi332 – 3376Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YLEX-ray1.70A1-338[»]
ProteinModelPortaliP80357.
SMRiP80357. Positions 1-338.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80357.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108T2Z. Bacteria.
COG3138. LUCA.
HOGENOMiHOG000258231.
KOiK00673.
OMAiETDHYID.
OrthoDBiEOG6XQ3H4.
PhylomeDBiP80357.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR017651. Arg/Orn_succinylTfrase_asu.
IPR007041. Arg_succinylTrfase_AstA/AruG.
[Graphical view]
PfamiPF04958. AstA. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR03245. arg_AOST_alph. 1 hit.
TIGR03243. arg_catab_AOST. 1 hit.

Sequencei

Sequence statusi: Complete.

P80357-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVMRPAQAA DLPQVQRLAA DSPVGVTSLP DDAERLRDKI LASEASFAAE
60 70 80 90 100
VSYNGEESYF FVLEDSASGE LVGCSAIVAS AGFSEPFYSF RNETFVHASR
110 120 130 140 150
SLSIHNKIHV LSLCHDLTGN SLLTSFYVQR DLVQSVYAEL NSRGRLLFMA
160 170 180 190 200
SHPERFADAV VVEIVGYSDE QGESPFWNAV GRNFFDLNYI EAEKLSGLKS
210 220 230 240 250
RTFLAELMPH YPIYVPLLPD AAQESMGQVH PRAQITFDIL MREGFETDNY
260 270 280 290 300
IDIFDGGPTL HARTSGIRSI AQSRVVPVKI GEAPKSGRPY LVTNGQLQDF
310 320 330
RAVVLDLDWA PGKPVALSVE AAEALGVGEG ASVRLVAV
Length:338
Mass (Da):36,931
Last modified:July 15, 1999 - v2
Checksum:i7152BB5EC220F719
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011922 Genomic DNA. Translation: AAC46010.1.
AE004091 Genomic DNA. Translation: AAG04285.1.
PIRiA83533.
RefSeqiNP_249587.1. NC_002516.2.
WP_003085950.1. NZ_ASJY01000130.1.

Genome annotation databases

EnsemblBacteriaiAAG04285; AAG04285; PA0896.
GeneIDi879437.
KEGGipae:PA0896.
PATRICi19836078. VBIPseAer58763_0931.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011922 Genomic DNA. Translation: AAC46010.1.
AE004091 Genomic DNA. Translation: AAG04285.1.
PIRiA83533.
RefSeqiNP_249587.1. NC_002516.2.
WP_003085950.1. NZ_ASJY01000130.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YLEX-ray1.70A1-338[»]
ProteinModelPortaliP80357.
SMRiP80357. Positions 1-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0896.

Proteomic databases

PaxDbiP80357.

Protocols and materials databases

DNASUi879437.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04285; AAG04285; PA0896.
GeneIDi879437.
KEGGipae:PA0896.
PATRICi19836078. VBIPseAer58763_0931.

Organism-specific databases

PseudoCAPiPA0896.

Phylogenomic databases

eggNOGiENOG4108T2Z. Bacteria.
COG3138. LUCA.
HOGENOMiHOG000258231.
KOiK00673.
OMAiETDHYID.
OrthoDBiEOG6XQ3H4.
PhylomeDBiP80357.

Enzyme and pathway databases

UniPathwayiUPA00185; UER00279.
BioCyciMetaCyc:MONOMER-11524.

Miscellaneous databases

EvolutionaryTraceiP80357.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR017651. Arg/Orn_succinylTfrase_asu.
IPR007041. Arg_succinylTrfase_AstA/AruG.
[Graphical view]
PfamiPF04958. AstA. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR03245. arg_AOST_alph. 1 hit.
TIGR03243. arg_catab_AOST. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the aru genes encoding enzymes of the catabolic arginine succinyltransferase pathway in Pseudomonas aeruginosa."
    Itoh Y.
    J. Bacteriol. 179:7280-7290(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "Purification and properties of a succinyltransferase from Pseudomonas aeruginosa specific for both arginine and ornithine."
    Tricot C., Vander Wauven C., Wattiez R., Falmagne P., Stalon V.
    Eur. J. Biochem. 224:853-861(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-11.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiASTA_PSEAE
AccessioniPrimary (citable) accession number: P80357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1999
Last modified: November 11, 2015
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.