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P80339

- RL32_THET8

UniProt

P80339 - RL32_THET8

Protein

50S ribosomal protein L32

Gene

rpmF

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Found on the solvent side of the large subunit.

    GO - Molecular functioni

    1. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. translation Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-439-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L32
    Gene namesi
    Name:rpmF
    Synonyms:rpl32
    Ordered Locus Names:TTHA0418
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. large ribosomal subunit Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 605950S ribosomal protein L32PRO_0000172427Add
    BLAST

    Interactioni

    Subunit structurei

    Part of the 50S ribosomal subunit.

    Protein-protein interaction databases

    STRINGi300852.TTHA0418.

    Structurei

    Secondary structure

    1
    60
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi13 – 197
    Helixi20 – 223
    Beta strandi31 – 333
    Turni34 – 363
    Beta strandi38 – 403
    Beta strandi42 – 454
    Turni47 – 493
    Beta strandi52 – 543
    Beta strandi55 – 573

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YL3X-ray5.5051-60[»]
    2HGJX-ray5.0041-60[»]
    2HGQX-ray5.5041-60[»]
    2HGUX-ray4.5141-60[»]
    2J01X-ray2.8052-60[»]
    2J03X-ray2.8052-60[»]
    2V47X-ray3.5052-60[»]
    2V49X-ray3.5052-60[»]
    2WDIX-ray3.3051-60[»]
    2WDJX-ray3.3051-60[»]
    2WDLX-ray3.5051-60[»]
    2WDNX-ray3.5051-60[»]
    2WH2X-ray3.4551-60[»]
    2WH4X-ray3.4551-60[»]
    2WRJX-ray3.6051-60[»]
    2WRLX-ray3.6051-60[»]
    2WROX-ray3.6051-60[»]
    2WRRX-ray3.6051-60[»]
    2X9SX-ray3.1051-60[»]
    2X9UX-ray3.1051-60[»]
    2XG0X-ray3.2051-60[»]
    2XG2X-ray3.2051-60[»]
    2XQEX-ray3.1051-60[»]
    2XTGelectron microscopy7.8051-60[»]
    2XUXelectron microscopy7.6051-60[»]
    2Y0VX-ray3.1051-60[»]
    2Y0XX-ray3.1051-60[»]
    2Y0ZX-ray3.1051-60[»]
    2Y11X-ray3.1051-60[»]
    2Y13X-ray3.1051-60[»]
    2Y15X-ray3.1051-60[»]
    2Y17X-ray3.1051-60[»]
    2Y19X-ray3.1051-60[»]
    3FINelectron microscopy6.4052-60[»]
    3HUXX-ray3.1051-60[»]
    3HUZX-ray3.1051-60[»]
    3I8FX-ray3.1051-60[»]
    3I8IX-ray3.1051-60[»]
    3I9CX-ray3.5051-60[»]
    3I9EX-ray3.5051-60[»]
    3KIRX-ray3.3051-60[»]
    3KITX-ray3.3051-60[»]
    3KIWX-ray3.6051-60[»]
    3KIYX-ray3.6051-60[»]
    3KNIX-ray3.0051-60[»]
    3KNKX-ray3.0051-60[»]
    3KNMX-ray3.4551-60[»]
    3KNOX-ray3.4551-60[»]
    3OH5X-ray3.0051-60[»]
    3OH7X-ray3.0051-60[»]
    3OHJX-ray3.0051-60[»]
    3OHKX-ray3.0051-60[»]
    3OHZX-ray3.0051-60[»]
    3OI1X-ray3.0051-60[»]
    3OI3X-ray3.1051-60[»]
    3OI5X-ray3.1051-60[»]
    3TVEX-ray3.1052-60[»]
    3TVHX-ray3.1052-60[»]
    3UXQX-ray3.2051-60[»]
    3UXRX-ray3.2051-60[»]
    3UYEX-ray3.0051-60[»]
    3UYGX-ray3.0051-60[»]
    3UZ1X-ray3.3051-60[»]
    3UZ2X-ray3.3051-60[»]
    3UZ8X-ray3.0051-60[»]
    3UZ9X-ray3.0051-60[»]
    3UZFX-ray3.3051-60[»]
    3UZHX-ray3.3051-60[»]
    3UZKX-ray3.3051-60[»]
    3UZNX-ray3.3051-60[»]
    3V23X-ray3.0051-60[»]
    3V25X-ray3.0051-60[»]
    3V27X-ray3.1051-60[»]
    3V29X-ray3.1051-60[»]
    3V2DX-ray2.7051-60[»]
    3V2FX-ray2.7051-60[»]
    3V6WX-ray3.9051-60[»]
    3V6XX-ray3.9051-60[»]
    3ZN9X-ray3.1051-60[»]
    3ZNEX-ray3.1051-60[»]
    3ZVPX-ray3.8051-60[»]
    4ABSX-ray3.1051-60[»]
    4B8GX-ray3.7051-60[»]
    4B8IX-ray3.7051-60[»]
    4BTDX-ray2.9552-60[»]
    4BYCX-ray3.3551-60[»]
    4BYEX-ray3.3551-60[»]
    4DHAX-ray3.2051-60[»]
    4DHCX-ray3.2051-60[»]
    4EJBX-ray3.5251-60[»]
    4EJCX-ray3.5251-60[»]
    4G5LX-ray3.3051-60[»]
    4G5NX-ray3.3051-60[»]
    4G5UX-ray3.1051-60[»]
    4G5WX-ray3.1051-60[»]
    4JUXX-ray2.8651-60[»]
    4K0MX-ray3.3052-60[»]
    4K0QX-ray3.3052-60[»]
    4NVVX-ray3.0051-60[»]
    4NVXX-ray3.0051-60[»]
    4NVZX-ray3.1051-60[»]
    4NW1X-ray3.1051-60[»]
    ProteinModelPortaliP80339.
    SMRiP80339. Positions 2-60.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP80339.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L32P family.Curated

    Phylogenomic databases

    eggNOGiCOG0333.
    HOGENOMiHOG000040268.
    KOiK02911.
    OMAiRTHFKLA.
    OrthoDBiEOG6VMTT4.
    PhylomeDBiP80339.

    Family and domain databases

    HAMAPiMF_00340. Ribosomal_L32.
    InterProiIPR002677. Ribosomal_L32p.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view]
    PfamiPF01783. Ribosomal_L32p. 1 hit.
    [Graphical view]
    SUPFAMiSSF57829. SSF57829. 1 hit.
    TIGRFAMsiTIGR01031. rpmF_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P80339-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKHPVPKKK TSKARRDARR SHHALTPPTL VPCPECKAMK PPHTVCPECG   50
    YYAGRKVLEV 60
    Length:60
    Mass (Da):6,705
    Last modified:January 23, 2007 - v5
    Checksum:i3B972CB90FF2DE59
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171D → A AA sequence (PubMed:11154066)Curated
    Sequence conflicti32 – 332PC → VP AA sequence 1 PublicationCurated
    Sequence conflicti36 – 361C → I AA sequence 1 PublicationCurated
    Sequence conflicti41 – 411P → H AA sequence 1 PublicationCurated

    Mass spectrometryi

    Molecular mass is 6575 Da from positions 2 - 60. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008226 Genomic DNA. Translation: BAD70241.1.
    RefSeqiYP_143684.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD70241; BAD70241; BAD70241.
    GeneIDi3168304.
    KEGGittj:TTHA0418.
    PATRICi23955807. VBITheThe93045_0418.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008226 Genomic DNA. Translation: BAD70241.1 .
    RefSeqi YP_143684.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YL3 X-ray 5.50 5 1-60 [» ]
    2HGJ X-ray 5.00 4 1-60 [» ]
    2HGQ X-ray 5.50 4 1-60 [» ]
    2HGU X-ray 4.51 4 1-60 [» ]
    2J01 X-ray 2.80 5 2-60 [» ]
    2J03 X-ray 2.80 5 2-60 [» ]
    2V47 X-ray 3.50 5 2-60 [» ]
    2V49 X-ray 3.50 5 2-60 [» ]
    2WDI X-ray 3.30 5 1-60 [» ]
    2WDJ X-ray 3.30 5 1-60 [» ]
    2WDL X-ray 3.50 5 1-60 [» ]
    2WDN X-ray 3.50 5 1-60 [» ]
    2WH2 X-ray 3.45 5 1-60 [» ]
    2WH4 X-ray 3.45 5 1-60 [» ]
    2WRJ X-ray 3.60 5 1-60 [» ]
    2WRL X-ray 3.60 5 1-60 [» ]
    2WRO X-ray 3.60 5 1-60 [» ]
    2WRR X-ray 3.60 5 1-60 [» ]
    2X9S X-ray 3.10 5 1-60 [» ]
    2X9U X-ray 3.10 5 1-60 [» ]
    2XG0 X-ray 3.20 5 1-60 [» ]
    2XG2 X-ray 3.20 5 1-60 [» ]
    2XQE X-ray 3.10 5 1-60 [» ]
    2XTG electron microscopy 7.80 5 1-60 [» ]
    2XUX electron microscopy 7.60 5 1-60 [» ]
    2Y0V X-ray 3.10 5 1-60 [» ]
    2Y0X X-ray 3.10 5 1-60 [» ]
    2Y0Z X-ray 3.10 5 1-60 [» ]
    2Y11 X-ray 3.10 5 1-60 [» ]
    2Y13 X-ray 3.10 5 1-60 [» ]
    2Y15 X-ray 3.10 5 1-60 [» ]
    2Y17 X-ray 3.10 5 1-60 [» ]
    2Y19 X-ray 3.10 5 1-60 [» ]
    3FIN electron microscopy 6.40 5 2-60 [» ]
    3HUX X-ray 3.10 5 1-60 [» ]
    3HUZ X-ray 3.10 5 1-60 [» ]
    3I8F X-ray 3.10 5 1-60 [» ]
    3I8I X-ray 3.10 5 1-60 [» ]
    3I9C X-ray 3.50 5 1-60 [» ]
    3I9E X-ray 3.50 5 1-60 [» ]
    3KIR X-ray 3.30 5 1-60 [» ]
    3KIT X-ray 3.30 5 1-60 [» ]
    3KIW X-ray 3.60 5 1-60 [» ]
    3KIY X-ray 3.60 5 1-60 [» ]
    3KNI X-ray 3.00 5 1-60 [» ]
    3KNK X-ray 3.00 5 1-60 [» ]
    3KNM X-ray 3.45 5 1-60 [» ]
    3KNO X-ray 3.45 5 1-60 [» ]
    3OH5 X-ray 3.00 5 1-60 [» ]
    3OH7 X-ray 3.00 5 1-60 [» ]
    3OHJ X-ray 3.00 5 1-60 [» ]
    3OHK X-ray 3.00 5 1-60 [» ]
    3OHZ X-ray 3.00 5 1-60 [» ]
    3OI1 X-ray 3.00 5 1-60 [» ]
    3OI3 X-ray 3.10 5 1-60 [» ]
    3OI5 X-ray 3.10 5 1-60 [» ]
    3TVE X-ray 3.10 5 2-60 [» ]
    3TVH X-ray 3.10 5 2-60 [» ]
    3UXQ X-ray 3.20 5 1-60 [» ]
    3UXR X-ray 3.20 5 1-60 [» ]
    3UYE X-ray 3.00 5 1-60 [» ]
    3UYG X-ray 3.00 5 1-60 [» ]
    3UZ1 X-ray 3.30 5 1-60 [» ]
    3UZ2 X-ray 3.30 5 1-60 [» ]
    3UZ8 X-ray 3.00 5 1-60 [» ]
    3UZ9 X-ray 3.00 5 1-60 [» ]
    3UZF X-ray 3.30 5 1-60 [» ]
    3UZH X-ray 3.30 5 1-60 [» ]
    3UZK X-ray 3.30 5 1-60 [» ]
    3UZN X-ray 3.30 5 1-60 [» ]
    3V23 X-ray 3.00 5 1-60 [» ]
    3V25 X-ray 3.00 5 1-60 [» ]
    3V27 X-ray 3.10 5 1-60 [» ]
    3V29 X-ray 3.10 5 1-60 [» ]
    3V2D X-ray 2.70 5 1-60 [» ]
    3V2F X-ray 2.70 5 1-60 [» ]
    3V6W X-ray 3.90 5 1-60 [» ]
    3V6X X-ray 3.90 5 1-60 [» ]
    3ZN9 X-ray 3.10 5 1-60 [» ]
    3ZNE X-ray 3.10 5 1-60 [» ]
    3ZVP X-ray 3.80 5 1-60 [» ]
    4ABS X-ray 3.10 5 1-60 [» ]
    4B8G X-ray 3.70 5 1-60 [» ]
    4B8I X-ray 3.70 5 1-60 [» ]
    4BTD X-ray 2.95 5 2-60 [» ]
    4BYC X-ray 3.35 5 1-60 [» ]
    4BYE X-ray 3.35 5 1-60 [» ]
    4DHA X-ray 3.20 5 1-60 [» ]
    4DHC X-ray 3.20 5 1-60 [» ]
    4EJB X-ray 3.52 5 1-60 [» ]
    4EJC X-ray 3.52 5 1-60 [» ]
    4G5L X-ray 3.30 5 1-60 [» ]
    4G5N X-ray 3.30 5 1-60 [» ]
    4G5U X-ray 3.10 5 1-60 [» ]
    4G5W X-ray 3.10 5 1-60 [» ]
    4JUX X-ray 2.86 5 1-60 [» ]
    4K0M X-ray 3.30 5 2-60 [» ]
    4K0Q X-ray 3.30 5 2-60 [» ]
    4NVV X-ray 3.00 5 1-60 [» ]
    4NVX X-ray 3.00 5 1-60 [» ]
    4NVZ X-ray 3.10 5 1-60 [» ]
    4NW1 X-ray 3.10 5 1-60 [» ]
    ProteinModelPortali P80339.
    SMRi P80339. Positions 2-60.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 300852.TTHA0418.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD70241 ; BAD70241 ; BAD70241 .
    GeneIDi 3168304.
    KEGGi ttj:TTHA0418.
    PATRICi 23955807. VBITheThe93045_0418.

    Phylogenomic databases

    eggNOGi COG0333.
    HOGENOMi HOG000040268.
    KOi K02911.
    OMAi RTHFKLA.
    OrthoDBi EOG6VMTT4.
    PhylomeDBi P80339.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-439-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P80339.

    Family and domain databases

    HAMAPi MF_00340. Ribosomal_L32.
    InterProi IPR002677. Ribosomal_L32p.
    IPR011332. Ribosomal_zn-bd.
    [Graphical view ]
    Pfami PF01783. Ribosomal_L32p. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57829. SSF57829. 1 hit.
    TIGRFAMsi TIGR01031. rpmF_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.
    2. "The isolation and complete amino acid sequence of the ribosomal protein L36 from Thermus thermophilus and its zinc-binding motif."
      Boysen R.I., Lorenz S., Kim J.S., Schroeder W.F.K.J., Erdmann V.A.
      Endocyt. Cell Res. 11:41-58(1995)
      Cited for: PROTEIN SEQUENCE OF 2-41.
    3. "Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
      Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
      Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-17.
    4. "Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
      Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
      Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
    5. Cited for: STRUCTURE OF THE RIBOSOME.

    Entry informationi

    Entry nameiRL32_THET8
    AccessioniPrimary (citable) accession number: P80339
    Secondary accession number(s): O05480, Q5SL75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1994
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 110 of the entry and version 5 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3