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P80319

- DHE3_PYRFU

UniProt

P80319 - DHE3_PYRFU

Protein

Glutamate dehydrogenase

Gene

gdhA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei105 – 1051

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi220 – 2267NADSequence Analysis

    GO - Molecular functioni

    1. glutamate dehydrogenase [NAD(P)+] activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular amino acid metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD, NADP

    Enzyme and pathway databases

    SABIO-RKP80319.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate dehydrogenase (EC:1.4.1.3)
    Short name:
    GDH
    Gene namesi
    Name:gdhA
    Synonyms:gdh
    Ordered Locus Names:PF1602
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 420420Glutamate dehydrogenasePRO_0000182757Add
    BLAST

    Proteomic databases

    PRIDEiP80319.

    Interactioni

    Subunit structurei

    Homohexamer.

    Protein-protein interaction databases

    STRINGi186497.PF1602.

    Structurei

    Secondary structure

    1
    420
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 1712
    Helixi18 – 203
    Helixi25 – 317
    Beta strandi35 – 4511
    Beta strandi51 – 6212
    Beta strandi66 – 694
    Beta strandi72 – 743
    Helixi80 – 9617
    Beta strandi102 – 1098
    Helixi112 – 1143
    Helixi117 – 13115
    Helixi132 – 1343
    Turni137 – 1393
    Helixi150 – 16415
    Helixi170 – 1734
    Helixi179 – 1813
    Turni185 – 1895
    Helixi190 – 20516
    Beta strandi215 – 2195
    Helixi223 – 23412
    Beta strandi239 – 2446
    Beta strandi249 – 2568
    Helixi258 – 26811
    Beta strandi269 – 2713
    Beta strandi277 – 2804
    Helixi282 – 2876
    Beta strandi291 – 2955
    Helixi306 – 3094
    Beta strandi313 – 3164
    Beta strandi319 – 3213
    Helixi325 – 3339
    Beta strandi337 – 3393
    Helixi341 – 3444
    Helixi347 – 36115
    Helixi367 – 39125
    Helixi396 – 41419

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GTMX-ray2.20A/B/C2-420[»]
    ProteinModelPortaliP80319.
    SMRiP80319. Positions 5-420.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP80319.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0334.
    HOGENOMiHOG000243801.
    KOiK00261.
    OMAiFNFDRAK.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P80319-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEQDPYEIV IKQLERAAQY MEISEEALEF LKRPQRIVEV TIPVEMDDGS    50
    VKVFTGFRVQ HNWARGPTKG GIRWHPEETL STVKALAAWM TWKTAVMDLP 100
    YGGGKGGIIV DPKKLSDREK ERLARGYIRA IYDVISPYED IPAPDVYTNP 150
    QIMAWMMDEY ETISRRKTPA FGIITGKPLS IGGSLGRIEA TARGASYTIR 200
    EAAKVLGWDT LKGKTIAIQG YGNAGYYLAK IMSEDFGMKV VAVSDSKGGI 250
    YNPDGLNADE VLKWKNEHGS VKDFPGATNI TNEELLELEV DVLAPAAIEE 300
    VITKKNADNI KAKIVAEVAN GPVTPEADEI LFEKGILQIP DFLCNAGGVT 350
    VSYFEWVQNI TGYYWTIEEV RERLDKKMTK AFYDVYNIAK EKNIHMRDAA 400
    YVVAVQRVYQ AMLDRGWVKH 420
    Length:420
    Mass (Da):47,114
    Last modified:February 1, 1996 - v2
    Checksum:i673DB20F8764A93C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti88 – 892AW → WA AA sequence (PubMed:8060497)Curated
    Sequence conflicti366 – 3661T → K AA sequence (PubMed:8060497)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97860 Genomic DNA. Translation: AAA83390.1.
    AE009950 Genomic DNA. Translation: AAL81726.1.
    PIRiT46971. JN0854.
    RefSeqiNP_579331.1. NC_003413.1.
    WP_011012748.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL81726; AAL81726; PF1602.
    GeneIDi1469478.
    KEGGipfu:PF1602.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97860 Genomic DNA. Translation: AAA83390.1 .
    AE009950 Genomic DNA. Translation: AAL81726.1 .
    PIRi T46971. JN0854.
    RefSeqi NP_579331.1. NC_003413.1.
    WP_011012748.1. NC_003413.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GTM X-ray 2.20 A/B/C 2-420 [» ]
    ProteinModelPortali P80319.
    SMRi P80319. Positions 5-420.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 186497.PF1602.

    Proteomic databases

    PRIDEi P80319.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL81726 ; AAL81726 ; PF1602 .
    GeneIDi 1469478.
    KEGGi pfu:PF1602.

    Phylogenomic databases

    eggNOGi COG0334.
    HOGENOMi HOG000243801.
    KOi K00261.
    OMAi FNFDRAK.

    Enzyme and pathway databases

    SABIO-RK P80319.

    Miscellaneous databases

    EvolutionaryTracei P80319.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The glutamate dehydrogenase-encoding gene of the hyperthermophilic archaeon Pyrococcus furiosus: sequence, transcription and analysis of the deduced amino acid sequence."
      Eggen R.I.L., Geerling A.C.M., Waldkoetter K., Antranikian G., de Vos W.M.
      Gene 132:143-148(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    2. "The amino acid sequence of glutamate dehydrogenase from Pyrococcus furiosus, a hyperthermophilic archaebacterium."
      Maras B., Valiante S., Chiaraluce R., Consalvi V., Politi L., de Rosa M., Bossa F., Scandurra R., Barra D.
      J. Protein Chem. 13:253-259(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    3. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    4. "The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures."
      Yip K.S.P., Stillman T.J., Britton K.L., Artymiuk P.J., Baker P.J., Sedelnikova S.E., Engel P.C., Pasquo A., Chiaraluce R., Consalvi V., Scandurra R., Rice D.W.
      Structure 3:1147-1158(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

    Entry informationi

    Entry nameiDHE3_PYRFU
    AccessioniPrimary (citable) accession number: P80319
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3