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Protein

Glutamate dehydrogenase

Gene

gdhA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1051

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi220 – 226NADSequence analysis7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD, NADP

Enzyme and pathway databases

BRENDAi1.4.1.3. 5243.
SABIO-RKP80319.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate dehydrogenase (EC:1.4.1.3)
Short name:
GDH
Gene namesi
Name:gdhA
Synonyms:gdh
Ordered Locus Names:PF1602
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001827571 – 420Glutamate dehydrogenaseAdd BLAST420

Proteomic databases

PRIDEiP80319.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi186497.PF1602.

Structurei

Secondary structure

1420
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 17Combined sources12
Helixi18 – 20Combined sources3
Helixi25 – 31Combined sources7
Beta strandi35 – 45Combined sources11
Beta strandi51 – 62Combined sources12
Beta strandi66 – 69Combined sources4
Beta strandi72 – 74Combined sources3
Helixi80 – 96Combined sources17
Beta strandi102 – 109Combined sources8
Helixi112 – 114Combined sources3
Helixi117 – 131Combined sources15
Helixi132 – 134Combined sources3
Turni137 – 139Combined sources3
Helixi150 – 164Combined sources15
Helixi170 – 173Combined sources4
Helixi179 – 181Combined sources3
Turni185 – 189Combined sources5
Helixi190 – 205Combined sources16
Beta strandi215 – 219Combined sources5
Helixi223 – 234Combined sources12
Beta strandi239 – 244Combined sources6
Beta strandi249 – 256Combined sources8
Helixi258 – 268Combined sources11
Beta strandi269 – 271Combined sources3
Beta strandi277 – 280Combined sources4
Helixi282 – 287Combined sources6
Beta strandi291 – 295Combined sources5
Helixi306 – 309Combined sources4
Beta strandi313 – 316Combined sources4
Beta strandi319 – 321Combined sources3
Helixi325 – 333Combined sources9
Beta strandi337 – 339Combined sources3
Helixi341 – 344Combined sources4
Helixi347 – 361Combined sources15
Helixi367 – 391Combined sources25
Helixi396 – 414Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GTMX-ray2.20A/B/C2-420[»]
ProteinModelPortaliP80319.
SMRiP80319.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80319.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01352. Archaea.
COG0334. LUCA.
HOGENOMiHOG000243801.
KOiK00261.
OMAiMENVITE.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P80319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEQDPYEIV IKQLERAAQY MEISEEALEF LKRPQRIVEV TIPVEMDDGS
60 70 80 90 100
VKVFTGFRVQ HNWARGPTKG GIRWHPEETL STVKALAAWM TWKTAVMDLP
110 120 130 140 150
YGGGKGGIIV DPKKLSDREK ERLARGYIRA IYDVISPYED IPAPDVYTNP
160 170 180 190 200
QIMAWMMDEY ETISRRKTPA FGIITGKPLS IGGSLGRIEA TARGASYTIR
210 220 230 240 250
EAAKVLGWDT LKGKTIAIQG YGNAGYYLAK IMSEDFGMKV VAVSDSKGGI
260 270 280 290 300
YNPDGLNADE VLKWKNEHGS VKDFPGATNI TNEELLELEV DVLAPAAIEE
310 320 330 340 350
VITKKNADNI KAKIVAEVAN GPVTPEADEI LFEKGILQIP DFLCNAGGVT
360 370 380 390 400
VSYFEWVQNI TGYYWTIEEV RERLDKKMTK AFYDVYNIAK EKNIHMRDAA
410 420
YVVAVQRVYQ AMLDRGWVKH
Length:420
Mass (Da):47,114
Last modified:February 1, 1996 - v2
Checksum:i673DB20F8764A93C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88 – 89AW → WA AA sequence (PubMed:8060497).Curated2
Sequence conflicti366T → K AA sequence (PubMed:8060497).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97860 Genomic DNA. Translation: AAA83390.1.
AE009950 Genomic DNA. Translation: AAL81726.1.
PIRiT46971. JN0854.
RefSeqiWP_011012748.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81726; AAL81726; PF1602.
GeneIDi1469478.
KEGGipfu:PF1602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97860 Genomic DNA. Translation: AAA83390.1.
AE009950 Genomic DNA. Translation: AAL81726.1.
PIRiT46971. JN0854.
RefSeqiWP_011012748.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GTMX-ray2.20A/B/C2-420[»]
ProteinModelPortaliP80319.
SMRiP80319.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1602.

Proteomic databases

PRIDEiP80319.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81726; AAL81726; PF1602.
GeneIDi1469478.
KEGGipfu:PF1602.

Phylogenomic databases

eggNOGiarCOG01352. Archaea.
COG0334. LUCA.
HOGENOMiHOG000243801.
KOiK00261.
OMAiMENVITE.

Enzyme and pathway databases

BRENDAi1.4.1.3. 5243.
SABIO-RKP80319.

Miscellaneous databases

EvolutionaryTraceiP80319.

Family and domain databases

CDDicd01076. NAD_bind_1_Glu_DH. 1 hit.
Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR033524. Glu/Leu/Phe/Val_DH_AS.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
IPR033922. NAD_bind_Glu_DH.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHE3_PYRFU
AccessioniPrimary (citable) accession number: P80319
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.