Reviewed, UniProtKB/Swiss-Prot P80319 (DHE3_PYRFU)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase
      Short name=GDH
    EC=1.4.1.3

Gene names

Name:	gdhA
Synonyms:	gdh
Ordered Locus Names:	PF1602

Organism

Pyrococcus furiosus [Complete proteome] [HAMAP]

Taxonomic identifier

2261 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NAD(P)(+) = 2-oxoglutarate + NH(3) + NAD(P)H.
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 420

420

Glutamate dehydrogenase

PRO_0000182757

Regions

Nucleotide binding

220 – 226

7

NAD Potential

Sites

Active site

105

1

Experimental info

Sequence conflict

88 – 89

2

AW → WA AA sequence Ref.2

Sequence conflict

366

1

T → K AA sequence Ref.2

Secondary structure

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420

Helix Strand Turn

Details...

Helix

6 – 17

12

Helix

18 – 20

3

Helix

25 – 31

7

Beta strand

35 – 45

11

Beta strand

51 – 62

12

Beta strand

66 – 69

4

Beta strand

72 – 74

3

Helix

80 – 96

17

Beta strand

102 – 109

8

Helix

112 – 114

3

Helix

117 – 131

15

Helix

132 – 134

3

Turn

137 – 139

3

Helix

150 – 164

15

Helix

170 – 173

4

Helix

179 – 181

3

Turn

185 – 189

5

Helix

190 – 205

16

Beta strand

215 – 219

5

Helix

223 – 234

12

Beta strand

239 – 244

6

Beta strand

249 – 256

8

Helix

258 – 268

11

Beta strand

269 – 271

3

Beta strand

277 – 280

4

Helix

282 – 287

6

Beta strand

291 – 295

5

Helix

306 – 309

4

Beta strand

313 – 316

4

Beta strand

319 – 321

3

Helix

325 – 333

9

Beta strand

337 – 339

3

Helix

341 – 344

4

Helix

347 – 361

15

Helix

367 – 391

25

Helix

396 – 414

19

Sequences

	Sequence		Length	Mass (Da)
	P80319-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 673DB20F8764A93C	FASTA	420	47,114
	10 20 30 40 50 60 MVEQDPYEIV IKQLERAAQY MEISEEALEF LKRPQRIVEV TIPVEMDDGS VKVFTGFRVQ 70 80 90 100 110 120 HNWARGPTKG GIRWHPEETL STVKALAAWM TWKTAVMDLP YGGGKGGIIV DPKKLSDREK 130 140 150 160 170 180 ERLARGYIRA IYDVISPYED IPAPDVYTNP QIMAWMMDEY ETISRRKTPA FGIITGKPLS 190 200 210 220 230 240 IGGSLGRIEA TARGASYTIR EAAKVLGWDT LKGKTIAIQG YGNAGYYLAK IMSEDFGMKV 250 260 270 280 290 300 VAVSDSKGGI YNPDGLNADE VLKWKNEHGS VKDFPGATNI TNEELLELEV DVLAPAAIEE 310 320 330 340 350 360 VITKKNADNI KAKIVAEVAN GPVTPEADEI LFEKGILQIP DFLCNAGGVT VSYFEWVQNI 370 380 390 400 410 420 TGYYWTIEEV RERLDKKMTK AFYDVYNIAK EKNIHMRDAA YVVAVQRVYQ AMLDRGWVKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The glutamate dehydrogenase-encoding gene of the hyperthermophilic archaeon Pyrococcus furiosus: sequence, transcription and analysis of the deduced amino acid sequence." Eggen R.I.L., Geerling A.C.M., Waldkoetter K., Antranikian G., de Vos W.M. Gene 132:143-148(1993) [PubMed: 8406037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]	"The amino acid sequence of glutamate dehydrogenase from Pyrococcus furiosus, a hyperthermophilic archaebacterium." Maras B., Valiante S., Chiaraluce R., Consalvi V., Politi L., de Rosa M., Bossa F., Scandurra R., Barra D. J. Protein Chem. 13:253-259(1994) [PubMed: 8060497] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]	"The complete sequence of the Pyrococcus furiosus genome." Weiss R.B., Dunn D.M., Robb F.T., Brown J.R. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[4]	"The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures." Yip K.S.P., Stillman T.J., Britton K.L., Artymiuk P.J., Baker P.J., Sedelnikova S.E., Engel P.C., Pasquo A., Chiaraluce R., Consalvi V., Scandurra R., Rice D.W. Structure 3:1147-1158(1995) [PubMed: 8591026] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M97860 Genomic DNA. Translation: AAA83390.1.
AE010260 Genomic DNA. Translation: AAL81726.1.

PIR

JN0854. T46971.

RefSeq

NP_579331.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GTM	X-ray	2.20	A/B/C	2-420	[»]

ModBase

Search...

Genome annotation databases

GeneID

1469478.

GenomeReviews

Gene locus PF1602 in contig AE009950_GR.

KEGG

pfu:PF1602.

NMPDR

fig|186497.1.peg.1649.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P80319.

Family and domain databases

InterPro

IPR006095. Glu/Leu/Phe/Val_DHase.
IPR006096. Glu/Leu/Phe/Val_DHase_C.
IPR006097. Glu/Leu/Phe/Val_DHase_dimer.
IPR014362. Glu_DHase.
IPR016040. NAD(P)-bd.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR11606:SF2. GLFV_DH. 1 hit.

Pfam

PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]

PIRSF

PIRSF000185. Glu_DH. 1 hit.

PRINTS

PR00082. GLFDHDRGNASE.

PROSITE

PS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P80319.

Entry information

Entry name

DHE3_PYRFU

Accession

Primary (citable) accession number: P80319

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 1, 1996
Last modified:	November 25, 2008
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)