Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

T-complex protein 1 subunit epsilon

Gene

Cct5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-390471 Association of TriC/CCT with target proteins during biosynthesis
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyi

Protein namesi
Recommended name:
T-complex protein 1 subunit epsilon
Short name:
TCP-1-epsilon
Alternative name(s):
CCT-epsilon
Gene namesi
Name:Cct5
Synonyms:Ccte, Kiaa0098
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:107185 Cct5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001283472 – 541T-complex protein 1 subunit epsilonAdd BLAST540

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki20Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei26PhosphoserineBy similarity1
Cross-linki210Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki214Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki265Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki275Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki279Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei346PhosphoserineBy similarity1
Cross-linki392Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei539PhosphoserineBy similarity1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP80316
MaxQBiP80316
PaxDbiP80316
PeptideAtlasiP80316
PRIDEiP80316

2D gel databases

REPRODUCTION-2DPAGEiIPI00116279
P80316
SWISS-2DPAGEiP80316

PTM databases

iPTMnetiP80316
PhosphoSitePlusiP80316
SwissPalmiP80316

Expressioni

Gene expression databases

BgeeiENSMUSG00000022234
CleanExiMM_CCT5
ExpressionAtlasiP80316 baseline and differential
GenevisibleiP80316 MM

Interactioni

Subunit structurei

Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8 (By similarity). Interacts with DNAAF4.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ThegQ9JMB12EBI-772379,EBI-1390549

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198568, 11 interactors
CORUMiP80316
IntActiP80316, 11 interactors
MINTiP80316
STRINGi10090.ENSMUSP00000022842

Structurei

3D structure databases

ProteinModelPortaliP80316
SMRiP80316
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TCP-1 chaperonin family.Curated

Phylogenomic databases

eggNOGiKOG0357 Eukaryota
COG0459 LUCA
GeneTreeiENSGT00550000074988
HOVERGENiHBG106507
InParanoidiP80316
KOiK09497
OMAiYATDEYG
OrthoDBiEOG091G05G4
PhylomeDBiP80316
TreeFamiTF105638

Family and domain databases

CDDicd03339 TCP1_epsilon, 1 hit
Gene3Di1.10.560.10, 2 hits
3.30.260.10, 2 hits
3.50.7.10, 1 hit
InterProiView protein in InterPro
IPR012718 Chap_CCT_epsi
IPR017998 Chaperone_TCP-1
IPR002194 Chaperonin_TCP-1_CS
IPR002423 Cpn60/TCP-1
IPR027409 GroEL-like_apical_dom_sf
IPR027413 GROEL-like_equatorial_sf
IPR027410 TCP-1-like_intermed_sf
PANTHERiPTHR11353:SF178 PTHR11353:SF178, 1 hit
PfamiView protein in Pfam
PF00118 Cpn60_TCP1, 1 hit
PRINTSiPR00304 TCOMPLEXTCP1
SUPFAMiSSF48592 SSF48592, 2 hits
SSF52029 SSF52029, 1 hit
SSF54849 SSF54849, 2 hits
TIGRFAMsiTIGR02343 chap_CCT_epsi, 1 hit
PROSITEiView protein in PROSITE
PS00750 TCP1_1, 1 hit
PS00751 TCP1_2, 1 hit
PS00995 TCP1_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80316-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVGTLAFD EYGRPFLIIK DQDRKSRLMG LEALKSHIMA AKAVANTMRT
60 70 80 90 100
SLGPNGLDKM MVDKDGDVTI TNDGATILSM MDVDHQIAKL MVELSKSQDD
110 120 130 140 150
EIGDGTTGVV VLAGALLEEA EQLLDRGIHP IRIADGYEQA ARIAIQHLDK
160 170 180 190 200
ISDKVLVDIN NPEPLIQTAK TTLGSKVINS CHRQMAEIAV NAVLTVADME
210 220 230 240 250
RRDVDFELIK VEGKVGGRLE DTKLIKGVIV DKDFSHPQMP KKVVDAKIAI
260 270 280 290 300
LTCPFEPPKP KTKHKLDVMS VEDYKALQKY EKEKFEEMIK QIKETGANLA
310 320 330 340 350
ICQWGFDDEA NHLLLQNGLP AVRWVGGPEI ELIAIATGGR IVPRFSELTS
360 370 380 390 400
EKLGFAGVVQ EISFGTTKDK MLVIEKCKNS RAVTIFIRGG NKMIIEEAKR
410 420 430 440 450
SLHDALCVIR NLIRDNRVVY GGGAAEISCA LAVSQEADKC PTLEQYAMRA
460 470 480 490 500
FADALEVIPM ALSENSGMNP IQTMTEVRAR QVKESNPALG IDCLHKGSND
510 520 530 540
MQYQHVIETL IGKKQQISLA TQMVRMILKI DDIRKPGESE E
Length:541
Mass (Da):59,624
Last modified:June 1, 1994 - v1
Checksum:iE05316785ADB7612
GO

Sequence cautioni

The sequence BAC97866 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27R → H in BAE23007 (PubMed:16141072).Curated1
Sequence conflicti48M → I in BAE27146 (PubMed:16141072).Curated1
Sequence conflicti74G → D in BAE32250 (PubMed:16141072).Curated1
Sequence conflicti125D → E in BAE27146 (PubMed:16141072).Curated1
Sequence conflicti191N → D in BAE39906 (PubMed:16141072).Curated1
Sequence conflicti493C → Y in BAE29518 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z31555 mRNA Translation: CAA83430.1
AB022158 Genomic DNA Translation: BAA81876.1
AK129056 mRNA Translation: BAC97866.1 Different initiation.
AK088184 mRNA Translation: BAC40194.1
AK133857 mRNA Translation: BAE21891.1
AK136493 mRNA Translation: BAE23007.1
AK145445 mRNA Translation: BAE26441.1
AK146405 mRNA Translation: BAE27146.1
AK146425 mRNA Translation: BAE27159.1
AK150390 mRNA Translation: BAE29518.1
AK153911 mRNA Translation: BAE32250.1
AK167895 mRNA Translation: BAE39906.1
BC094427 mRNA Translation: AAH94427.1
CCDSiCCDS27411.1
PIRiS43061
RefSeqiNP_001334964.1, NM_001348035.1
NP_031663.1, NM_007637.3
UniGeneiMm.282158

Genome annotation databases

EnsembliENSMUST00000022842; ENSMUSP00000022842; ENSMUSG00000022234
GeneIDi12465
KEGGimmu:12465
UCSCiuc007vkj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTCPE_MOUSE
AccessioniPrimary (citable) accession number: P80316
Secondary accession number(s): Q3TIE0
, Q3U530, Q3UCU4, Q3UJK9, Q3UWA9, Q542K3, Q6ZQJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health