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Protein

T-complex protein 1 subunit delta

Gene

Cct4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-390471. Association of TriC/CCT with target proteins during biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
T-complex protein 1 subunit delta
Short name:
TCP-1-delta
Alternative name(s):
A45
CCT-delta
Gene namesi
Name:Cct4
Synonyms:Cctd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:104689. Cct4.

Subcellular locationi

GO - Cellular componenti

  • cell body Source: MGI
  • centrosome Source: MGI
  • chaperonin-containing T-complex Source: MGI
  • cilium Source: UniProtKB-KW
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • melanosome Source: UniProtKB-SubCell
  • microtubule Source: MGI
  • nucleoplasm Source: MGI
  • zona pellucida receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 539538T-complex protein 1 subunit deltaPRO_0000128333Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei21 – 211N6-acetyllysineCombined sources
Modified residuei288 – 2881N6-acetyllysineBy similarity
Modified residuei302 – 3021N6-acetyllysineBy similarity
Modified residuei319 – 3191N6-acetyllysineCombined sources
Modified residuei326 – 3261N6-acetyllysineBy similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP80315.
PaxDbiP80315.
PRIDEiP80315.

2D gel databases

REPRODUCTION-2DPAGEP80315.

PTM databases

iPTMnetiP80315.
PhosphoSiteiP80315.
SwissPalmiP80315.

Expressioni

Gene expression databases

BgeeiP80315.
CleanExiMM_CCT4.
ExpressionAtlasiP80315. baseline and differential.
GenevisibleiP80315. MM.

Interactioni

Subunit structurei

Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8 (By similarity). Interacts with DYX1C1.By similarity1 Publication

Protein-protein interaction databases

BioGridi198567. 52 interactions.
IntActiP80315. 52 interactions.
MINTiMINT-1862659.
STRINGi10090.ENSMUSP00000133523.

Structurei

3D structure databases

ProteinModelPortaliP80315.
SMRiP80315. Positions 19-245, 383-539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TCP-1 chaperonin family.Curated

Phylogenomic databases

eggNOGiKOG0358. Eukaryota.
COG0459. LUCA.
HOGENOMiHOG000226735.
HOVERGENiHBG106507.
InParanoidiP80315.
KOiK09496.
OMAiAYCVRAY.
OrthoDBiEOG722J8B.
PhylomeDBiP80315.
TreeFamiTF106332.

Family and domain databases

Gene3Di1.10.560.10. 2 hits.
3.30.260.10. 2 hits.
3.50.7.10. 1 hit.
InterProiIPR012717. Chap_CCT_delta.
IPR017998. Chaperone_TCP-1.
IPR002194. Chaperonin_TCP-1_CS.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
IPR027410. TCP-1-like_intermed.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00304. TCOMPLEXTCP1.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02342. chap_CCT_delta. 1 hit.
PROSITEiPS00750. TCP1_1. 1 hit.
PS00751. TCP1_2. 1 hit.
PS00995. TCP1_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80315-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPENVASRSG APTAGPGSRG KSAYQDRDKP AQIRFSNISA AKAVADAIRT
60 70 80 90 100
SLGPKGMDKM IQDGKGDVTI TNDGATILKQ MQVLHPAARM LVELSKAQDI
110 120 130 140 150
EAGDGTTSVV IIAGSLLDSC TKLLQKGIHP TIISESFQKA LEKGLEILTD
160 170 180 190 200
MSRPVQLSDR ETLLNSATTS LNSKVVSQYS SLLSPMSVNA VMKVIDPATA
210 220 230 240 250
TSVDLRDIKI VKKLGGTIDD CELVEGLVLT QKVANSGITR VEKAKIGLIQ
260 270 280 290 300
FCLSAPKTDM DNQIVVSDYA QMDRVLREER AYILNLVKQI KKTGCNVLLI
310 320 330 340 350
QKSILRDALS DLALHFLNKM KIMVVKDVER EDIEFICKTI GTKPVAHIDQ
360 370 380 390 400
FTADMLGSAE LAEEVSLNGS GKLFKITGCT SPGKTVTIVV RGSNKLVIEE
410 420 430 440 450
AERSIHDALC VIRCLVKKRA LIAGGGAPEI ELALRLTEYS RTLSGMESYC
460 470 480 490 500
VRAFADAMEV IPSTLAENAG LNPISTVTEL RNRHAQGEKT TGINVRKGGI
510 520 530
SNILEEMVVQ PLLVSVSALT LATETVRSIL KIDDVVNTR
Length:539
Mass (Da):58,066
Last modified:January 23, 2007 - v3
Checksum:iB3EBD470D1C7FABC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti405 – 4051I → V in AAA37418 (PubMed:7919332).Curated
Sequence conflicti443 – 4431L → P in AAA37418 (PubMed:7919332).Curated
Sequence conflicti453 – 4531A → R in AAA37418 (PubMed:7919332).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25913 mRNA. Translation: AAA37418.1.
Z31554 mRNA. Translation: CAA83429.1.
AB022157 Genomic DNA. Translation: BAA81875.1.
BC054773 mRNA. Translation: AAH54773.1.
CCDSiCCDS24474.1.
PIRiS43060.
RefSeqiNP_033967.1. NM_009837.1.
UniGeneiMm.296985.
Mm.394074.
Mm.441628.

Genome annotation databases

EnsembliENSMUST00000173867; ENSMUSP00000133523; ENSMUSG00000007739.
GeneIDi12464.
KEGGimmu:12464.
UCSCiuc007ien.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25913 mRNA. Translation: AAA37418.1.
Z31554 mRNA. Translation: CAA83429.1.
AB022157 Genomic DNA. Translation: BAA81875.1.
BC054773 mRNA. Translation: AAH54773.1.
CCDSiCCDS24474.1.
PIRiS43060.
RefSeqiNP_033967.1. NM_009837.1.
UniGeneiMm.296985.
Mm.394074.
Mm.441628.

3D structure databases

ProteinModelPortaliP80315.
SMRiP80315. Positions 19-245, 383-539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198567. 52 interactions.
IntActiP80315. 52 interactions.
MINTiMINT-1862659.
STRINGi10090.ENSMUSP00000133523.

PTM databases

iPTMnetiP80315.
PhosphoSiteiP80315.
SwissPalmiP80315.

2D gel databases

REPRODUCTION-2DPAGEP80315.

Proteomic databases

EPDiP80315.
PaxDbiP80315.
PRIDEiP80315.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000173867; ENSMUSP00000133523; ENSMUSG00000007739.
GeneIDi12464.
KEGGimmu:12464.
UCSCiuc007ien.1. mouse.

Organism-specific databases

CTDi10575.
MGIiMGI:104689. Cct4.

Phylogenomic databases

eggNOGiKOG0358. Eukaryota.
COG0459. LUCA.
HOGENOMiHOG000226735.
HOVERGENiHBG106507.
InParanoidiP80315.
KOiK09496.
OMAiAYCVRAY.
OrthoDBiEOG722J8B.
PhylomeDBiP80315.
TreeFamiTF106332.

Enzyme and pathway databases

ReactomeiR-MMU-390471. Association of TriC/CCT with target proteins during biosynthesis.

Miscellaneous databases

NextBioi281330.
PROiP80315.
SOURCEiSearch...

Gene expression databases

BgeeiP80315.
CleanExiMM_CCT4.
ExpressionAtlasiP80315. baseline and differential.
GenevisibleiP80315. MM.

Family and domain databases

Gene3Di1.10.560.10. 2 hits.
3.30.260.10. 2 hits.
3.50.7.10. 1 hit.
InterProiIPR012717. Chap_CCT_delta.
IPR017998. Chaperone_TCP-1.
IPR002194. Chaperonin_TCP-1_CS.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
IPR027410. TCP-1-like_intermed.
[Graphical view]
PfamiPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSiPR00304. TCOMPLEXTCP1.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02342. chap_CCT_delta. 1 hit.
PROSITEiPS00750. TCP1_1. 1 hit.
PS00751. TCP1_2. 1 hit.
PS00995. TCP1_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a new mammalian chaperonin gene from a multipotent hematopoietic progenitor clone."
    Xie X., Palacios R.
    Blood 84:2171-2174(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Bone marrow.
  2. "Identification of six Tcp-1-related genes encoding divergent subunits of the TCP-1-containing chaperonin."
    Kubota H., Hynes G., Carne A., Ashworth A., Willison K.R.
    Curr. Biol. 4:89-99(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/Sv.
  3. "Structures and co-regulated expression of the genes encoding mouse cytosolic chaperonin CCT subunits."
    Kubota H., Yokota S., Yanagi H., Yura T.
    Eur. J. Biochem. 262:492-500(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  5. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 60-79; 127-139; 144-206; 246-274; 281-288; 293-302; 307-319; 327-338; 396-403 AND 420-435, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J and OF1.
    Tissue: Brain and Hippocampus.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  7. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-21 AND LYS-319, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  8. Cited for: INTERACTION WITH DYX1C1.
  9. Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTCPD_MOUSE
AccessioniPrimary (citable) accession number: P80315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: April 13, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.