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Protein

Nucleobindin-2

Gene

NUCB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-binding protein. May have a role in calcium homeostasis.
Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance (By similarity).By similarity

Miscellaneous

NEFA stands for N=DNA-binding; EF=EF-hand; A=Acidic region.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi171 – 223By similarityAdd BLAST53
Calcium bindingi254 – 2651Add BLAST12
Calcium bindingi306 – 3172Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: ProtInc
  • DNA binding Source: ProtInc

Keywordsi

Molecular functionDNA-binding
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleobindin-2
Alternative name(s):
DNA-binding protein NEFA
Gastric cancer antigen Zg4
Prepronesfatin
Cleaved into the following chain:
Gene namesi
Name:NUCB2
Synonyms:NEFA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Unplaced

Organism-specific databases

HGNCiHGNC:8044. NUCB2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi4925.
PharmGKBiPA31826.

Polymorphism and mutation databases

BioMutaiNUCB2.
DMDMi224471846.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisCombined sourcesAdd BLAST24
ChainiPRO_000000416525 – 420Nucleobindin-2Add BLAST396
ChainiPRO_000041981925 – 106Nesfatin-1Add BLAST82

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei257PhosphoserineCombined sources1
Modified residuei332PhosphoserineCombined sources1

Keywords - PTMi

Cleavage on pair of basic residues, Phosphoprotein

Proteomic databases

EPDiP80303.
MaxQBiP80303.
PaxDbiP80303.
PeptideAtlasiP80303.
PRIDEiP80303.

PTM databases

iPTMnetiP80303.
PhosphoSitePlusiP80303.

Miscellaneous databases

PMAP-CutDBiP80303.

Expressioni

Tissue specificityi

Predominantly expressed in spleen, testis and normal stomach.1 Publication

Gene expression databases

CleanExiHS_NUCB2.

Organism-specific databases

HPAiHPA008395.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
GADD45AP245222EBI-2296670,EBI-448167

Protein-protein interaction databases

BioGridi110979. 29 interactors.
IntActiP80303. 13 interactors.
MINTiMINT-2804938.
STRINGi9606.ENSP00000436455.

Structurei

3D structure databases

ProteinModelPortaliP80303.
SMRiP80303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini241 – 276EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini293 – 328EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni213 – 420Binds to necdinBy similarityAdd BLAST208

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi282 – 293Asp/Glu-rich (acidic)Add BLAST12

Sequence similaritiesi

Belongs to the nucleobindin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3866. Eukaryota.
ENOG410XW7D. LUCA.
HOGENOMiHOG000007216.
HOVERGENiHBG052685.
InParanoidiP80303.
KOiK20371.
PhylomeDBiP80303.
TreeFamiTF323218.

Family and domain databases

InterProiView protein in InterPro
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028813. NUCB2.
PANTHERiPTHR19237:SF26. PTHR19237:SF26. 1 hit.
PfamiView protein in Pfam
PF13499. EF-hand_7. 1 hit.
SMARTiView protein in SMART
SM00054. EFh. 2 hits.
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P80303-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRWRTILLQY CFLLITCLLT ALEAVPIDID KTKVQNIHPV ESAKIEPPDT
60 70 80 90 100
GLYYDEYLKQ VIDVLETDKH FREKLQKADI EEIKSGRLSK ELDLVSHHVR
110 120 130 140 150
TKLDELKRQE VGRLRMLIKA KLDSLQDIGM DHQALLKQFD HLNHLNPDKF
160 170 180 190 200
ESTDLDMLIK AATSDLEHYD KTRHEEFKKY EMMKEHERRE YLKTLNEEKR
210 220 230 240 250
KEEESKFEEM KKKHENHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF
260 270 280 290 300
KLHDVNSDGF LDEQELEALF TKELEKVYDP KNEEDDMVEM EEERLRMREH
310 320 330 340 350
VMSEVDTNKD RLVTLEEFLK ATEKKEFLEP DSWETLDQQQ FFTEEELKEY
360 370 380 390 400
ENIIALQENE LKKKADELQK QKEELQRQHD QLEAQKLEYH QVIQQMEQKK
410 420
LQQGIPPSGP AGELKFEPHI
Length:420
Mass (Da):50,196
Last modified:March 3, 2009 - v2
Checksum:i5BA7459AB725A1A3
GO
Isoform 2 (identifier: P80303-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     419-420: HI → RV

Note: No experimental confirmation available.
Show »
Length:420
Mass (Da):50,201
Checksum:i4922A59AB725A1A3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150F → L in BAF84196 (PubMed:14702039).Curated1

Polymorphismi

Deletion of Gln-402 is frequent.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_068746303S → NCombined sources6 PublicationsCorresponds to variant dbSNP:rs2521998Ensembl.1
Natural variantiVAR_024399338Q → E. Corresponds to variant dbSNP:rs757081Ensembl.1
Natural variantiVAR_020923402Missing 2 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036450419 – 420HI → RV in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76732 mRNA. Translation: CAA54148.1.
AF052642 mRNA. Translation: AAC06300.1.
AF052643 mRNA. Translation: AAC06301.1.
AF052644 mRNA. Translation: AAC06302.1.
AF450266 mRNA. Translation: AAM73810.1.
AB478625 Genomic DNA. Translation: BAJ09615.1.
AK291507 mRNA. Translation: BAF84196.1.
AC107956 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68439.1.
CH471064 Genomic DNA. Translation: EAW68440.1.
CCDSiCCDS41623.1. [P80303-1]
PIRiS55272.
RefSeqiNP_005004.1. NM_005013.2.
XP_016873295.1. XM_017017806.1.
XP_016873296.1. XM_017017807.1.
XP_016873297.1. XM_017017808.1.
XP_016873298.1. XM_017017809.1.
XP_016873299.1. XM_017017810.1.
XP_016873300.1. XM_017017811.1.
XP_016873301.1. XM_017017812.1.
XP_016873302.1. XM_017017813.1.
XP_016873303.1. XM_017017814.1.
XP_016873304.1. XM_017017815.1.
XP_016873305.1. XM_017017816.1.
XP_016873306.1. XM_017017817.1.
XP_016873307.1. XM_017017818.1.
XP_016873308.1. XM_017017819.1.
UniGeneiHs.654599.

Genome annotation databases

GeneIDi4925.
KEGGihsa:4925.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNUCB2_HUMAN
AccessioniPrimary (citable) accession number: P80303
Secondary accession number(s): A8K642, D3DQX5, Q8NFT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: March 3, 2009
Last modified: August 30, 2017
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families