Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P80303

- NUCB2_HUMAN

UniProt

P80303 - NUCB2_HUMAN

Protein

Nucleobindin-2

Gene

NUCB2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (03 Mar 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Calcium-binding protein. May have a role in calcium homeostasis.
    Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi171 – 22353By similarityAdd
    BLAST
    Calcium bindingi254 – 265121Add
    BLAST
    Calcium bindingi306 – 317122Add
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: ProtInc
    2. DNA binding Source: ProtInc
    3. protein binding Source: IntAct

    Keywords - Ligandi

    Calcium, DNA-binding, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleobindin-2
    Alternative name(s):
    DNA-binding protein NEFA
    Gastric cancer antigen Zg4
    Prepronesfatin
    Cleaved into the following chain:
    Gene namesi
    Name:NUCB2
    Synonyms:NEFA
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:8044. NUCB2.

    Subcellular locationi

    Golgi apparatus 1 Publication. Membrane 1 Publication; Peripheral membrane protein 1 Publication. Cytoplasm 1 Publication. Secreted 1 Publication. Endoplasmic reticulum By similarity. Nucleus envelope By similarity
    Note: Golgi retention is mediated by its N-terminal region.

    GO - Cellular componenti

    1. cytosol Source: ProtInc
    2. endoplasmic reticulum Source: UniProtKB-SubCell
    3. endoplasmic reticulum-Golgi intermediate compartment Source: UniProtKB
    4. extracellular space Source: UniProt
    5. extracellular vesicular exosome Source: UniProt
    6. Golgi apparatus Source: HPA
    7. nuclear outer membrane Source: Ensembl
    8. plasma membrane Source: ProtInc

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane, Nucleus, Secreted

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31826.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 420396Nucleobindin-2PRO_0000004165Add
    BLAST
    Chaini25 – 10682Nesfatin-1PRO_0000419819Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei257 – 2571Phosphoserine1 Publication
    Modified residuei332 – 3321Phosphoserine1 Publication

    Keywords - PTMi

    Cleavage on pair of basic residues, Phosphoprotein

    Proteomic databases

    MaxQBiP80303.
    PaxDbiP80303.
    PRIDEiP80303.

    PTM databases

    PhosphoSiteiP80303.

    Miscellaneous databases

    PMAP-CutDBP80303.

    Expressioni

    Tissue specificityi

    Predominantly expressed in spleen, testis and normal stomach.1 Publication

    Gene expression databases

    ArrayExpressiP80303.
    BgeeiP80303.
    CleanExiHS_NUCB2.
    GenevestigatoriP80303.

    Organism-specific databases

    HPAiCAB015931.
    HPA008395.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GADD45AP245222EBI-2296670,EBI-448167

    Protein-protein interaction databases

    BioGridi110979. 16 interactions.
    IntActiP80303. 11 interactions.
    MINTiMINT-2804938.
    STRINGi9606.ENSP00000320168.

    Structurei

    3D structure databases

    ProteinModelPortaliP80303.
    SMRiP80303. Positions 229-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini241 – 27636EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini293 – 32836EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni213 – 420208Binds to necdinBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi282 – 29312Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the nucleobindin family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG262100.
    HOGENOMiHOG000007216.
    HOVERGENiHBG052685.
    InParanoidiP80303.
    OMAiDHPKVNH.
    PhylomeDBiP80303.
    TreeFamiTF323218.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028813. NUCB2.
    [Graphical view]
    PANTHERiPTHR19237:SF22. PTHR19237:SF22. 1 hit.
    PfamiPF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P80303-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRWRTILLQY CFLLITCLLT ALEAVPIDID KTKVQNIHPV ESAKIEPPDT    50
    GLYYDEYLKQ VIDVLETDKH FREKLQKADI EEIKSGRLSK ELDLVSHHVR 100
    TKLDELKRQE VGRLRMLIKA KLDSLQDIGM DHQALLKQFD HLNHLNPDKF 150
    ESTDLDMLIK AATSDLEHYD KTRHEEFKKY EMMKEHERRE YLKTLNEEKR 200
    KEEESKFEEM KKKHENHPKV NHPGSKDQLK EVWEETDGLD PNDFDPKTFF 250
    KLHDVNSDGF LDEQELEALF TKELEKVYDP KNEEDDMVEM EEERLRMREH 300
    VMSEVDTNKD RLVTLEEFLK ATEKKEFLEP DSWETLDQQQ FFTEEELKEY 350
    ENIIALQENE LKKKADELQK QKEELQRQHD QLEAQKLEYH QVIQQMEQKK 400
    LQQGIPPSGP AGELKFEPHI 420
    Length:420
    Mass (Da):50,196
    Last modified:March 3, 2009 - v2
    Checksum:i5BA7459AB725A1A3
    GO
    Isoform 2 (identifier: P80303-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         419-420: HI → RV

    Note: No experimental confirmation available.

    Show »
    Length:420
    Mass (Da):50,201
    Checksum:i4922A59AB725A1A3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti150 – 1501F → L in BAF84196. (PubMed:14702039)Curated

    Polymorphismi

    Deletion of Gln-402 is frequent.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti303 – 3031S → N.7 Publications
    Corresponds to variant rs2521998 [ dbSNP | Ensembl ].
    VAR_068746
    Natural varianti338 – 3381Q → E.
    Corresponds to variant rs757081 [ dbSNP | Ensembl ].
    VAR_024399
    Natural varianti402 – 4021Missing.2 Publications
    VAR_020923

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei419 – 4202HI → RV in isoform 2. 1 PublicationVSP_036450

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76732 mRNA. Translation: CAA54148.1.
    AF052642 mRNA. Translation: AAC06300.1.
    AF052643 mRNA. Translation: AAC06301.1.
    AF052644 mRNA. Translation: AAC06302.1.
    AF450266 mRNA. Translation: AAM73810.1.
    AB478625 Genomic DNA. Translation: BAJ09615.1.
    AK291507 mRNA. Translation: BAF84196.1.
    AC107956 Genomic DNA. No translation available.
    CH471064 Genomic DNA. Translation: EAW68439.1.
    CH471064 Genomic DNA. Translation: EAW68440.1.
    CCDSiCCDS41623.1. [P80303-1]
    PIRiS55272.
    RefSeqiNP_005004.1. NM_005013.2.
    XP_005252998.2. XM_005252941.2.
    XP_005252999.2. XM_005252942.2.
    XP_005253000.2. XM_005252943.2.
    XP_005253001.2. XM_005252944.2.
    XP_005253002.2. XM_005252945.2.
    XP_005253004.2. XM_005252947.2.
    XP_005253005.2. XM_005252948.2.
    UniGeneiHs.654599.

    Genome annotation databases

    GeneIDi4925.
    KEGGihsa:4925.
    UCSCiuc001mms.1. human. [P80303-1]

    Polymorphism databases

    DMDMi224471846.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76732 mRNA. Translation: CAA54148.1 .
    AF052642 mRNA. Translation: AAC06300.1 .
    AF052643 mRNA. Translation: AAC06301.1 .
    AF052644 mRNA. Translation: AAC06302.1 .
    AF450266 mRNA. Translation: AAM73810.1 .
    AB478625 Genomic DNA. Translation: BAJ09615.1 .
    AK291507 mRNA. Translation: BAF84196.1 .
    AC107956 Genomic DNA. No translation available.
    CH471064 Genomic DNA. Translation: EAW68439.1 .
    CH471064 Genomic DNA. Translation: EAW68440.1 .
    CCDSi CCDS41623.1. [P80303-1 ]
    PIRi S55272.
    RefSeqi NP_005004.1. NM_005013.2.
    XP_005252998.2. XM_005252941.2.
    XP_005252999.2. XM_005252942.2.
    XP_005253000.2. XM_005252943.2.
    XP_005253001.2. XM_005252944.2.
    XP_005253002.2. XM_005252945.2.
    XP_005253004.2. XM_005252947.2.
    XP_005253005.2. XM_005252948.2.
    UniGenei Hs.654599.

    3D structure databases

    ProteinModelPortali P80303.
    SMRi P80303. Positions 229-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110979. 16 interactions.
    IntActi P80303. 11 interactions.
    MINTi MINT-2804938.
    STRINGi 9606.ENSP00000320168.

    PTM databases

    PhosphoSitei P80303.

    Polymorphism databases

    DMDMi 224471846.

    Proteomic databases

    MaxQBi P80303.
    PaxDbi P80303.
    PRIDEi P80303.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4925.
    KEGGi hsa:4925.
    UCSCi uc001mms.1. human. [P80303-1 ]

    Organism-specific databases

    CTDi 4925.
    GeneCardsi GC11P017229.
    HGNCi HGNC:8044. NUCB2.
    HPAi CAB015931.
    HPA008395.
    MIMi 608020. gene.
    neXtProti NX_P80303.
    PharmGKBi PA31826.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG262100.
    HOGENOMi HOG000007216.
    HOVERGENi HBG052685.
    InParanoidi P80303.
    OMAi DHPKVNH.
    PhylomeDBi P80303.
    TreeFami TF323218.

    Miscellaneous databases

    ChiTaRSi NUCB2. human.
    GeneWikii NUCB2.
    GenomeRNAii 4925.
    NextBioi 18967.
    PMAP-CutDB P80303.
    PROi P80303.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P80303.
    Bgeei P80303.
    CleanExi HS_NUCB2.
    Genevestigatori P80303.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR028813. NUCB2.
    [Graphical view ]
    PANTHERi PTHR19237:SF22. PTHR19237:SF22. 1 hit.
    Pfami PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Human protein NEFA, a novel DNA binding/EF-hand/leucine zipper protein. Molecular cloning and sequence analysis of the cDNA, isolation and characterization of the protein."
      Barnikol-Watanabe S., Gross N.A., Goetz H., Henkel T., Karabinos A., Kratzin H., Barnikol H.U., Hilschmann N.
      Biol. Chem. Hoppe-Seyler 375:497-512(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE, VARIANT ASN-303.
      Tissue: Blood.
    2. "Assembly of a high-resolution map of the Acadian Usher syndrome region and localization of the nuclear EF-hand acidic gene."
      Deangelis M.M., Doucet J.P., Drury S., Sherry S.T., Robichaux M.B., Den Z., Pelias M.Z., Ditta G.M., Keats B.J., Deininger P.L., Batzer M.A.
      Biochim. Biophys. Acta 1407:84-91(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT ASN-303.
    3. "Serological identification and expression analysis of gastric cancer-associated genes."
      Line A., Stengrevics A., Slucka Z., Li G., Jankevics E., Rees R.C.
      Br. J. Cancer 86:1824-1830(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, VARIANTS ASN-303 AND GLN-402 DEL.
    4. "Troglitazone, a ligand of peroxisome proliferator-activated receptor-gamma, stabilizes NUCB2 (Nesfatin) mRNA by activating the ERK1/2 pathway: isolation and characterization of the human NUCB2 gene."
      Yamada M., Horiguchi K., Umezawa R., Hashimoto K., Satoh T., Ozawa A., Shibusawa N., Monden T., Okada S., Shimizu H., Mori M.
      Endocrinology 151:2494-2503(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASN-303.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS ASN-303 AND GLN-402 DEL.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-303.
    8. "Golgi retention of human protein NEFA is mediated by its N-terminal Leu/Ile-rich region."
      Nesselhut J., Jurgan U., Onken E., Gotz H., Barnikol H.U., Hirschfeld G., Barnikol-Watanabe S., Hilschmann N.
      FEBS Lett. 509:469-475(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-257, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    11. Cited for: VARIANT [LARGE SCALE ANALYSIS] ASN-303, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNUCB2_HUMAN
    AccessioniPrimary (citable) accession number: P80303
    Secondary accession number(s): A8K642, D3DQX5, Q8NFT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: March 3, 2009
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    NEFA stands for N=DNA-binding; EF=EF-hand; A=Acidic region.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3