P80298 (BLAC_PROVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-lactamase EC=3.5.2.6 Alternative name(s): Penicillinase
Organism	Proteus vulgaris
Taxonomic identifier	585 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against cephalosporins.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subunit structure	Monomer.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	Beta-lactamase		PRO_0000195441
Regions
	Region	210 – 212	3	Substrate binding By similarity
Sites
	Active site	46	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80298 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: C6148E4F2500DDFF	FASTA	271	29,803
	10 20 30 40 50 60 NTNNTIEEQL STLEKYSQGR LGVALINTED NSQITYRGEE RFAMASTSKV MAVAAILKES 70 80 90 100 110 120 EKQAGLLDKN IIITKSDLVA YSPITEKHLA TGMSLAQLSA ATLQYSDNTA MNKILDYLGG 130 140 150 160 170 180 PSKVTQFARS INDVTYRLDR KEPELNTAIH GDPRDTTSPI AMAKSLQALT LGDALGQSQR 190 200 210 220 230 240 QQLVTWLKGN TTGDHSIKAG LPKHWIVGDK TGSGDYGTTN DIAVIWPKNH APLILVVYFT 250 260 270 QQEQDAKYRK DIIVKATEIV TKEFSNTSQK K

References

[1]	"Chromosomally encoded cephalosporin-hydrolyzing beta-lactamase of Proteus vulgaris RO104 belongs to Ambler's class A." Peduzzi J., Reynaud A., Barthelemy M., Baron P., Labia R. Biochim. Biophys. Acta 1207:31-39(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: RO104.

Cross-references

Sequence databases
PIR	S47620.
3D structure databases
ProteinModelPortal	P80298.
SMR	P80298. Positions 4-267.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL4782.

Entry information

Entry name

BLAC_PROVU

Accession

Primary (citable) accession number: P80298

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program