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Protein

Metallothionein-1X

Gene

MT1X

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi5 – 51Divalent metal cation; cluster B
Metal bindingi7 – 71Divalent metal cation; cluster B
Metal bindingi13 – 131Divalent metal cation; cluster B
Metal bindingi15 – 151Divalent metal cation; cluster B
Metal bindingi19 – 191Divalent metal cation; cluster B
Metal bindingi21 – 211Divalent metal cation; cluster B
Metal bindingi24 – 241Divalent metal cation; cluster B
Metal bindingi26 – 261Divalent metal cation; cluster B
Metal bindingi29 – 291Divalent metal cation; cluster B
Metal bindingi33 – 331Divalent metal cation; cluster A
Metal bindingi34 – 341Divalent metal cation; cluster A
Metal bindingi36 – 361Divalent metal cation; cluster A
Metal bindingi37 – 371Divalent metal cation; cluster A
Metal bindingi41 – 411Divalent metal cation; cluster A
Metal bindingi44 – 441Divalent metal cation; cluster A
Metal bindingi48 – 481Divalent metal cation; cluster A
Metal bindingi50 – 501Divalent metal cation; cluster A
Metal bindingi57 – 571Divalent metal cation; cluster A
Metal bindingi59 – 591Divalent metal cation; cluster A
Metal bindingi60 – 601Divalent metal cation; cluster A

GO - Molecular functioni

  1. metal ion binding Source: ProtInc
  2. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. cellular response to cadmium ion Source: UniProtKB
  2. cellular response to erythropoietin Source: UniProtKB
  3. cellular response to zinc ion Source: UniProtKB
  4. cellular zinc ion homeostasis Source: Ensembl
  5. detoxification of copper ion Source: Ensembl
  6. negative regulation of growth Source: UniProtKB
  7. nitric oxide mediated signal transduction Source: Ensembl
  8. response to metal ion Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

Cadmium, Copper, Metal-binding, Metal-thiolate cluster, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Metallothionein-1X
Short name:
MT-1X
Alternative name(s):
Metallothionein-IX
Short name:
MT-IX
Gene namesi
Name:MT1X
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:7405. MT1X.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
  3. perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31213.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6161Metallothionein-1XPRO_0000197243Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP80297.
PaxDbiP80297.
PRIDEiP80297.

PTM databases

PhosphoSiteiP80297.

Expressioni

Gene expression databases

BgeeiP80297.
CleanExiHS_MT1X.
ExpressionAtlasiP80297. baseline and differential.
GenevestigatoriP80297.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi110607. 6 interactions.
STRINGi9606.ENSP00000377995.

Structurei

3D structure databases

ProteinModelPortaliP80297.
SMRiP80297. Positions 1-61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2929BetaAdd
BLAST
Regioni30 – 6132AlphaAdd
BLAST

Domaini

Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG327133.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000236262.
HOVERGENiHBG094464.
InParanoidiP80297.
KOiK14739.
OMAiCACKEAS.
OrthoDBiEOG7PZS1X.
PhylomeDBiP80297.
TreeFamiTF336054.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P80297-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDPNCSCSPV GSCACAGSCK CKECKCTSCK KSCCSCCPVG CAKCAQGCIC
60
KGTSDKCSCC A
Length:61
Mass (Da):6,068
Last modified:February 1, 1994 - v1
Checksum:i381966F942E986B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76717 mRNA. Translation: CAA54136.1.
X65607 Genomic DNA. Translation: CAA46557.1.
AC026461 Genomic DNA. No translation available.
BC032338 mRNA. Translation: AAH32338.1.
S68956 mRNA. Translation: AAB30084.1.
CCDSiCCDS10768.1.
PIRiS47652.
RefSeqiNP_005943.1. NM_005952.3.
UniGeneiHs.374950.

Genome annotation databases

EnsembliENST00000394485; ENSP00000377995; ENSG00000187193.
GeneIDi4501.
KEGGihsa:4501.
UCSCiuc002ejy.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76717 mRNA. Translation: CAA54136.1.
X65607 Genomic DNA. Translation: CAA46557.1.
AC026461 Genomic DNA. No translation available.
BC032338 mRNA. Translation: AAH32338.1.
S68956 mRNA. Translation: AAB30084.1.
CCDSiCCDS10768.1.
PIRiS47652.
RefSeqiNP_005943.1. NM_005952.3.
UniGeneiHs.374950.

3D structure databases

ProteinModelPortaliP80297.
SMRiP80297. Positions 1-61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110607. 6 interactions.
STRINGi9606.ENSP00000377995.

PTM databases

PhosphoSiteiP80297.

Proteomic databases

MaxQBiP80297.
PaxDbiP80297.
PRIDEiP80297.

Protocols and materials databases

DNASUi4501.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394485; ENSP00000377995; ENSG00000187193.
GeneIDi4501.
KEGGihsa:4501.
UCSCiuc002ejy.3. human.

Organism-specific databases

CTDi4501.
GeneCardsiGC16P056719.
HGNCiHGNC:7405. MT1X.
MIMi156359. gene.
neXtProtiNX_P80297.
PharmGKBiPA31213.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG327133.
GeneTreeiENSGT00730000110883.
HOGENOMiHOG000236262.
HOVERGENiHBG094464.
InParanoidiP80297.
KOiK14739.
OMAiCACKEAS.
OrthoDBiEOG7PZS1X.
PhylomeDBiP80297.
TreeFamiTF336054.

Miscellaneous databases

ChiTaRSiMT1X. human.
GeneWikiiMT1X.
GenomeRNAii4501.
NextBioi17402.
PROiP80297.
SOURCEiSearch...

Gene expression databases

BgeeiP80297.
CleanExiHS_MT1X.
ExpressionAtlasiP80297. baseline and differential.
GenevestigatoriP80297.

Family and domain databases

Gene3Di4.10.10.10. 1 hit.
InterProiIPR017854. Metalthion_dom.
IPR023587. Metalthion_dom_vert.
IPR003019. Metalthion_sfam_euk.
IPR000006. Metalthion_vert.
IPR018064. Metalthion_vert_metal_BS.
[Graphical view]
PANTHERiPTHR23299. PTHR23299. 1 hit.
PfamiPF00131. Metallothio. 1 hit.
[Graphical view]
PRINTSiPR00860. MTVERTEBRATE.
SUPFAMiSSF57868. SSF57868. 1 hit.
PROSITEiPS00203. METALLOTHIONEIN_VRT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Soumillion A., van Weyenbergh J., de Ley M.
    Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. Hunziker P.E.
    Submitted (NOV-1993) to UniProtKB
    Cited for: PROTEIN SEQUENCE.
    Tissue: Liver.
  3. "Characterisation of six additional human metallothionein genes."
    Stennard F.A., Holloway A.F., Hamilton J., West A.K.
    Biochim. Biophys. Acta 1218:357-365(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Placenta.
  4. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  6. "Induction by zinc of specific metallothionein isoforms in human monocytes."
    Pauwels M., van Weyenbergh J., Soumillion A., Proost P., Ley M.
    Eur. J. Biochem. 220:105-110(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31.
  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMT1X_HUMAN
AccessioniPrimary (citable) accession number: P80297
Secondary accession number(s): A8MUC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: February 1, 1994
Last modified: January 7, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Metallothioneins
    Classification of metallothioneins and list of entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.