P80294 (MT1H_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Metallothionein-1H
Short name=MT-1H

Alternative name(s):
Metallothionein-0
Short name=MT-0
Metallothionein-IH
Short name=MT-IH

Gene names

Name:

MT1H

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	61 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Subunit structure	Monomer.
Domain	Class I metallothioneins contain 2 metal-binding domains: four divalent ions are chelated within cluster A of the alpha domain and are coordinated via cysteinyl thiolate bridges to 11 cysteine ligands. Cluster B, the corresponding region within the beta domain, can ligate three divalent ions to 9 cysteines.
Sequence similarities	Belongs to the metallothionein superfamily. Type 1 family.

Ontologies

Keywords
Ligand	Cadmium Copper Metal-binding Metal-thiolate cluster Zinc
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to cadmium ion Inferred from expression pattern PubMed 16827180. Source: UniProtKB cellular response to zinc ion Inferred from expression pattern PubMed 11168427Ref.7. Source: UniProtKB negative regulation of growth Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	zinc ion binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPINK7	P58062	3	EBI-296567,EBI-1182445

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 61	61	Metallothionein-1H		PRO_0000197239
Regions
	Region	1 – 29	29	Beta
	Region	30 – 61	32	Alpha
Sites
	Metal binding	5	1	Divalent metal cation; cluster B
	Metal binding	7	1	Divalent metal cation; cluster B
	Metal binding	13	1	Divalent metal cation; cluster B
	Metal binding	15	1	Divalent metal cation; cluster B
	Metal binding	19	1	Divalent metal cation; cluster B
	Metal binding	21	1	Divalent metal cation; cluster B
	Metal binding	24	1	Divalent metal cation; cluster B
	Metal binding	26	1	Divalent metal cation; cluster B
	Metal binding	29	1	Divalent metal cation; cluster B
	Metal binding	33	1	Divalent metal cation; cluster A
	Metal binding	34	1	Divalent metal cation; cluster A
	Metal binding	36	1	Divalent metal cation; cluster A
	Metal binding	37	1	Divalent metal cation; cluster A
	Metal binding	41	1	Divalent metal cation; cluster A
	Metal binding	44	1	Divalent metal cation; cluster A
	Metal binding	48	1	Divalent metal cation; cluster A
	Metal binding	50	1	Divalent metal cation; cluster A
	Metal binding	57	1	Divalent metal cation; cluster A
	Metal binding	59	1	Divalent metal cation; cluster A
	Metal binding	60	1	Divalent metal cation; cluster A
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P80294 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 03D866E4F437A2CC	FASTA	61	6,039
	10 20 30 40 50 60 MDPNCSCEAG GSCACAGSCK CKKCKCTSCK KSCCSCCPLG CAKCAQGCIC KGASEKCSCC A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Hunziker P.E. Submitted (NOV-1993) to UniProtKB Cited for: PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Cloning and specific polymerised-chain-reaction amplification of a third charge-separable human metallothionein isoform." Soumillion A., van Damme J., de Ley M. Eur. J. Biochem. 209:999-1004(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 3-22. Tissue: Embryonic liver.
[3]	"Characterisation of six additional human metallothionein genes." Stennard F.A., Holloway A.F., Hamilton J., West A.K. Biochim. Biophys. Acta 1218:357-365(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Pancreas and Prostate.
[7]	"Induction by zinc of specific metallothionein isoforms in human monocytes." Pauwels M., van Weyenbergh J., Soumillion A., Proost P., Ley M. Eur. J. Biochem. 220:105-110(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31, PROTEIN SEQUENCE OF 1-30. Tissue: Monocyte.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64834 Genomic DNA. Translation: CAA46046.1. X64177 mRNA. Translation: CAA45516.1. BT007171 mRNA. Translation: AAP35835.1. CH471092 Genomic DNA. Translation: EAW82884.1. BC008408 mRNA. Translation: AAH08408.1. BC059780 mRNA. Translation: AAH59780.1. BC146930 mRNA. Translation: AAI46931.1. BC146934 mRNA. Translation: AAI46935.1. S68948 mRNA. Translation: AAB30081.1.
IPI	IPI00008750.
PIR	S47651.
RefSeq	NP_005942.1. NM_005951.2.
UniGene	Hs.438462.
3D structure databases
ProteinModelPortal	P80294.
ModBase	Search...
Protein-protein interaction databases
IntAct	P80294. 2 interactions.
STRING	9606.ENSP00000330587.
Polymorphism databases
DMDM	462634.
Proteomic databases
PaxDb	P80294.
PRIDE	P80294.
Protocols and materials databases
DNASU	4496.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000332374; ENSP00000330587; ENSG00000205358.
GeneID	4496.
KEGG	hsa:4496.
UCSC	uc002ejw.3. human.
Organism-specific databases
CTD	4496.
GeneCards	GC16P056703.
H-InvDB	HIX0028598.
HGNC	HGNC:7400. MT1H.
HPA	CAB032277.
MIM	156354. gene.
neXtProt	NX_P80294.
PharmGKB	PA31205.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG138665.
HOGENOM	HOG000236262.
HOVERGEN	HBG094463.
InParanoid	P80294.
KO	K14739.
OMA	CACKEAS.
Gene expression databases
ArrayExpress	P80294.
Bgee	P80294.
CleanEx	HS_MT1H.
Genevestigator	P80294.
GermOnline	ENSG00000205358. Homo sapiens.
Family and domain databases
Gene3D	4.10.10.10. 1 hit.
InterPro	IPR017854. Metalthion_dom. IPR023587. Metalthion_dom_vert. IPR003019. Metalthion_sfam_euk. IPR000006. Metalthion_vert. IPR018064. Metalthion_vert_metal_BS. [Graphical view]
PANTHER	PTHR23299. PTHR23299. 1 hit.
Pfam	PF00131. Metallothio. 1 hit. [Graphical view]
PRINTS	PR00860. MTVERTEBRATE.
SUPFAM	SSF57868. Metallothionein_sfam. 1 hit.
PROSITE	PS00203. METALLOTHIONEIN_VRT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	17387.
SOURCE	Search...

Entry information

Entry name

MT1H_HUMAN

Accession

Primary (citable) accession number: P80294
Secondary accession number(s): B2RUY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.