P80287 (RNAS1_IGUIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ribonuclease pancreatic EC=3.1.27.5 Alternative name(s): RNase 1 RNase A
Organism	Iguana iguana (Common iguana)
Taxonomic identifier	8517 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Iguania › Iguanidae › Iguaninae › Iguana

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	Ribonuclease pancreatic		PRO_0000057203
Regions
	Region	40 – 44	5	Substrate binding By similarity
Sites
	Active site	10	1	Proton acceptor By similarity
	Active site	113	1	Proton donor By similarity
Amino acid modifications
	Modified residue	1	1	Pyrrolidone carboxylic acid
	Disulfide bond	25 ↔ 80		By similarity
	Disulfide bond	39 ↔ 91		By similarity
	Disulfide bond	57 ↔ 106		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P80287 [UniParc]. Last modified February 1, 1994. Version 1. Checksum: 6072FB5B7B15BD5A	FASTA	119	13,324
	10 20 30 40 50 60 QDWSSFQNKH IDYPETSASN PNAYCDLMMQ RRNLNPTKCK TRNTFVHASP SEIQQVCGSG 70 80 90 100 110 GTHYEDNLYD SNESFDLTDC KNVGGTAPSS CKYNGTPGTK RIRIACENNQ PVHFELVLS

References

[1]	"The amino acid sequence of iguana (Iguana iguana) pancreatic ribonuclease." Zhao W., Beintema J.J., Hofsteenge J. Eur. J. Biochem. 219:641-646(1994) [PubMed: 8307028] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	S41111.
3D structure databases
ProteinModelPortal	P80287.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008396.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_IGUIG

Accession

Primary (citable) accession number: P80287

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1994
Last sequence update:	February 1, 1994
Last modified:	September 21, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program