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P80284

- PDI_HORVU

UniProt

P80284 - PDI_HORVU

Protein

Protein disulfide-isomerase

Gene

PDI

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer.By similarity

    Catalytic activityi

    Catalyzes the rearrangement of -S-S- bonds in proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei67 – 671NucleophileBy similarity
    Sitei68 – 681Contributes to redox potential valueBy similarity
    Sitei69 – 691Contributes to redox potential valueBy similarity
    Active sitei70 – 701NucleophileBy similarity
    Sitei135 – 1351Lowers pKa of C-terminal Cys of first active siteBy similarity
    Active sitei411 – 4111NucleophileBy similarity
    Sitei412 – 4121Contributes to redox potential valueBy similarity
    Sitei413 – 4131Contributes to redox potential valueBy similarity
    Active sitei414 – 4141NucleophileBy similarity
    Sitei474 – 4741Lowers pKa of C-terminal Cys of second active siteBy similarity

    GO - Molecular functioni

    1. protein disulfide isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. embryo development Source: EnsemblPlants/Gramene
    3. regulation of programmed cell death Source: EnsemblPlants/Gramene
    4. response to cadmium ion Source: EnsemblPlants/Gramene
    5. response to endoplasmic reticulum stress Source: EnsemblPlants/Gramene
    6. response to salt stress Source: EnsemblPlants/Gramene
    7. response to zinc ion Source: EnsemblPlants/Gramene
    8. seed development Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Isomerase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein disulfide-isomerase (EC:5.3.4.1)
    Short name:
    PDI
    Alternative name(s):
    Endosperm protein E-1
    Gene namesi
    Name:PDI
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiP80284.

    Subcellular locationi

    Endoplasmic reticulum lumen PROSITE-ProRule annotation

    GO - Cellular componenti

    1. chloroplast Source: EnsemblPlants/Gramene
    2. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    3. Golgi apparatus Source: EnsemblPlants/Gramene
    4. lytic vacuole within protein storage vacuole Source: EnsemblPlants/Gramene
    5. plant-type cell wall Source: EnsemblPlants/Gramene
    6. plasma membrane Source: EnsemblPlants/Gramene
    7. thylakoid Source: EnsemblPlants/Gramene
    8. vacuolar membrane Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 25251 PublicationAdd
    BLAST
    Chaini26 – 513488Protein disulfide-isomerasePRO_0000034208Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi67 ↔ 70Redox-activePROSITE-ProRule annotation
    Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi411 ↔ 414Redox-activePROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriP80284.

    Interactioni

    Protein-protein interaction databases

    IntActiP80284. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP80284.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini26 – 149124Thioredoxin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini369 – 488120Thioredoxin 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi510 – 5134Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the protein disulfide isomerase family.Curated
    Contains 2 thioredoxin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Repeat, Signal

    Family and domain databases

    Gene3Di3.40.30.10. 3 hits.
    InterProiIPR005788. Disulphide_isomerase.
    IPR005792. Prot_disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view]
    PfamiPF00085. Thioredoxin. 2 hits.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 4 hits.
    TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
    TIGR01126. pdi_dom. 2 hits.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P80284-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAISKVWISL LLALAVVLSA PAARAEEAAA AEEAAAPEAV LTLHADNFDD    50
    AIGQHPFILV EFYAPWCGHC KSLAPEYEKA AQLLSKHDPA IVLAKVDAND 100
    EKNKPLAGKY EVQGFPTLKI FRNGGKSIQE YKGPREAEGI VEYLKKQVGP 150
    ASKEIKAPED ATYLEDGKIH IVGVFTEFSG PEFTNFLEVA EKLRSYYDFG 200
    HTVHANHLPR GDAAVERPVV RLFKPFDELV VDSKDFDVSA LEKFIDASST 250
    PKVVIFDKNP DNHPYLLKFF QSNAPKAMLF LNFSTGPFES FKSAYYGAVE 300
    EFSGKDVKFL IGDIESSQGA FQYFGLKVDQ APLILIQDGD SKKFLKEHVE 350
    AGQIVAWLKD YFDGKLTPFR KSEPIPEANN EPVKVVVADN VHDVVFKSGK 400
    NVLIEFYAPW CGHCKKLAPI LDEAAATLQS EEDVVIAKMD ATENDVPGEF 450
    DVQGYPTLYF VTPSGKKVSY EGGRTADEIV DYIRKNKETA GQAAAATEKA 500
    AEPAATEPLK DEL 513
    Length:513
    Mass (Da):56,463
    Last modified:October 1, 1996 - v2
    Checksum:i03BBAE2EE164F972
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L33250 mRNA. Translation: AAA70344.1.
    L33251 mRNA. Translation: AAA70345.1.
    L33252 mRNA. Translation: AAA70346.1.
    PIRiT05974.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L33250 mRNA. Translation: AAA70344.1 .
    L33251 mRNA. Translation: AAA70345.1 .
    L33252 mRNA. Translation: AAA70346.1 .
    PIRi T05974.

    3D structure databases

    ProteinModelPortali P80284.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P80284. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P80284.

    Gene expression databases

    Genevestigatori P80284.

    Family and domain databases

    Gene3Di 3.40.30.10. 3 hits.
    InterProi IPR005788. Disulphide_isomerase.
    IPR005792. Prot_disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view ]
    Pfami PF00085. Thioredoxin. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 4 hits.
    TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
    TIGR01126. pdi_dom. 2 hits.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and developmental expression of duplicated genes encoding protein disulfide isomerase in barley (Hordeum vulgare L.)."
      Chen F., Hayes P.M.
      Plant Physiol. 106:1705-1706(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Morex.
      Tissue: Ovary.
    2. "Separation of acidic barley endosperm proteins by two-dimensional electrophoresis."
      Flengsrud R.
      Electrophoresis 14:1060-1066(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 26-42.
      Strain: cv. H354-295-2-5.
      Tissue: Starchy endosperm.

    Entry informationi

    Entry nameiPDI_HORVU
    AccessioniPrimary (citable) accession number: P80284
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3