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P80284

- PDI_HORVU

UniProt

P80284 - PDI_HORVU

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Protein

Protein disulfide-isomerase

Gene

PDI

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671NucleophileBy similarity
Sitei68 – 681Contributes to redox potential valueBy similarity
Sitei69 – 691Contributes to redox potential valueBy similarity
Active sitei70 – 701NucleophileBy similarity
Sitei135 – 1351Lowers pKa of C-terminal Cys of first active siteBy similarity
Active sitei411 – 4111NucleophileBy similarity
Sitei412 – 4121Contributes to redox potential valueBy similarity
Sitei413 – 4131Contributes to redox potential valueBy similarity
Active sitei414 – 4141NucleophileBy similarity
Sitei474 – 4741Lowers pKa of C-terminal Cys of second active siteBy similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase (EC:5.3.4.1)
Short name:
PDI
Alternative name(s):
Endosperm protein E-1
Gene namesi
Name:PDI
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP80284.

Subcellular locationi

Endoplasmic reticulum lumen PROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 513488Protein disulfide-isomerasePRO_0000034208Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi67 ↔ 70Redox-activePROSITE-ProRule annotation
Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi411 ↔ 414Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Gene expression databases

GenevestigatoriP80284.

Interactioni

Protein-protein interaction databases

IntActiP80284. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP80284.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 149124Thioredoxin 1PROSITE-ProRule annotationAdd
BLAST
Domaini369 – 488120Thioredoxin 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi510 – 5134Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80284-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAISKVWISL LLALAVVLSA PAARAEEAAA AEEAAAPEAV LTLHADNFDD
60 70 80 90 100
AIGQHPFILV EFYAPWCGHC KSLAPEYEKA AQLLSKHDPA IVLAKVDAND
110 120 130 140 150
EKNKPLAGKY EVQGFPTLKI FRNGGKSIQE YKGPREAEGI VEYLKKQVGP
160 170 180 190 200
ASKEIKAPED ATYLEDGKIH IVGVFTEFSG PEFTNFLEVA EKLRSYYDFG
210 220 230 240 250
HTVHANHLPR GDAAVERPVV RLFKPFDELV VDSKDFDVSA LEKFIDASST
260 270 280 290 300
PKVVIFDKNP DNHPYLLKFF QSNAPKAMLF LNFSTGPFES FKSAYYGAVE
310 320 330 340 350
EFSGKDVKFL IGDIESSQGA FQYFGLKVDQ APLILIQDGD SKKFLKEHVE
360 370 380 390 400
AGQIVAWLKD YFDGKLTPFR KSEPIPEANN EPVKVVVADN VHDVVFKSGK
410 420 430 440 450
NVLIEFYAPW CGHCKKLAPI LDEAAATLQS EEDVVIAKMD ATENDVPGEF
460 470 480 490 500
DVQGYPTLYF VTPSGKKVSY EGGRTADEIV DYIRKNKETA GQAAAATEKA
510
AEPAATEPLK DEL
Length:513
Mass (Da):56,463
Last modified:October 1, 1996 - v2
Checksum:i03BBAE2EE164F972
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33250 mRNA. Translation: AAA70344.1.
L33251 mRNA. Translation: AAA70345.1.
L33252 mRNA. Translation: AAA70346.1.
PIRiT05974.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33250 mRNA. Translation: AAA70344.1 .
L33251 mRNA. Translation: AAA70345.1 .
L33252 mRNA. Translation: AAA70346.1 .
PIRi T05974.

3D structure databases

ProteinModelPortali P80284.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P80284. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P80284.

Gene expression databases

Genevestigatori P80284.

Family and domain databases

Gene3Di 3.40.30.10. 3 hits.
InterProi IPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 4 hits.
TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and developmental expression of duplicated genes encoding protein disulfide isomerase in barley (Hordeum vulgare L.)."
    Chen F., Hayes P.M.
    Plant Physiol. 106:1705-1706(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Morex.
    Tissue: Ovary.
  2. "Separation of acidic barley endosperm proteins by two-dimensional electrophoresis."
    Flengsrud R.
    Electrophoresis 14:1060-1066(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-42.
    Strain: cv. H354-295-2-5.
    Tissue: Starchy endosperm.

Entry informationi

Entry nameiPDI_HORVU
AccessioniPrimary (citable) accession number: P80284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3