SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P80284

- PDI_HORVU

UniProt

P80284 - PDI_HORVU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein disulfide-isomerase

Gene
PDI
Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer By similarity.

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Nucleophile By similarity
Sitei68 – 681Contributes to redox potential value By similarity
Sitei69 – 691Contributes to redox potential value By similarity
Active sitei70 – 701Nucleophile By similarity
Sitei135 – 1351Lowers pKa of C-terminal Cys of first active site By similarity
Active sitei411 – 4111Nucleophile By similarity
Sitei412 – 4121Contributes to redox potential value By similarity
Sitei413 – 4131Contributes to redox potential value By similarity
Active sitei414 – 4141Nucleophile By similarity
Sitei474 – 4741Lowers pKa of C-terminal Cys of second active site By similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. embryo development Source: EnsemblPlants/Gramene
  3. regulation of programmed cell death Source: EnsemblPlants/Gramene
  4. response to cadmium ion Source: EnsemblPlants/Gramene
  5. response to endoplasmic reticulum stress Source: EnsemblPlants/Gramene
  6. response to salt stress Source: EnsemblPlants/Gramene
  7. response to zinc ion Source: EnsemblPlants/Gramene
  8. seed development Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase (EC:5.3.4.1)
Short name:
PDI
Alternative name(s):
Endosperm protein E-1
Gene namesi
Name:PDI
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP80284.

Subcellular locationi

Endoplasmic reticulum lumen Reviewed prediction

GO - Cellular componenti

  1. chloroplast Source: EnsemblPlants/Gramene
  2. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  3. Golgi apparatus Source: EnsemblPlants/Gramene
  4. lytic vacuole within protein storage vacuole Source: EnsemblPlants/Gramene
  5. plant-type cell wall Source: EnsemblPlants/Gramene
  6. plasma membrane Source: EnsemblPlants/Gramene
  7. thylakoid Source: EnsemblPlants/Gramene
  8. vacuolar membrane Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 513488Protein disulfide-isomerasePRO_0000034208Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi67 ↔ 70Redox-active By similarity
Glycosylationi282 – 2821N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi411 ↔ 414Redox-active By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Gene expression databases

GenevestigatoriP80284.

Interactioni

Protein-protein interaction databases

IntActiP80284. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP80284.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 149124Thioredoxin 1Add
BLAST
Domaini369 – 488120Thioredoxin 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi510 – 5134Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Contains 2 thioredoxin domains.

Keywords - Domaini

Redox-active center, Repeat, Signal

Family and domain databases

Gene3Di3.40.30.10. 3 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80284-1 [UniParc]FASTAAdd to Basket

« Hide

MAISKVWISL LLALAVVLSA PAARAEEAAA AEEAAAPEAV LTLHADNFDD    50
AIGQHPFILV EFYAPWCGHC KSLAPEYEKA AQLLSKHDPA IVLAKVDAND 100
EKNKPLAGKY EVQGFPTLKI FRNGGKSIQE YKGPREAEGI VEYLKKQVGP 150
ASKEIKAPED ATYLEDGKIH IVGVFTEFSG PEFTNFLEVA EKLRSYYDFG 200
HTVHANHLPR GDAAVERPVV RLFKPFDELV VDSKDFDVSA LEKFIDASST 250
PKVVIFDKNP DNHPYLLKFF QSNAPKAMLF LNFSTGPFES FKSAYYGAVE 300
EFSGKDVKFL IGDIESSQGA FQYFGLKVDQ APLILIQDGD SKKFLKEHVE 350
AGQIVAWLKD YFDGKLTPFR KSEPIPEANN EPVKVVVADN VHDVVFKSGK 400
NVLIEFYAPW CGHCKKLAPI LDEAAATLQS EEDVVIAKMD ATENDVPGEF 450
DVQGYPTLYF VTPSGKKVSY EGGRTADEIV DYIRKNKETA GQAAAATEKA 500
AEPAATEPLK DEL 513
Length:513
Mass (Da):56,463
Last modified:October 1, 1996 - v2
Checksum:i03BBAE2EE164F972
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L33250 mRNA. Translation: AAA70344.1.
L33251 mRNA. Translation: AAA70345.1.
L33252 mRNA. Translation: AAA70346.1.
PIRiT05974.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L33250 mRNA. Translation: AAA70344.1 .
L33251 mRNA. Translation: AAA70345.1 .
L33252 mRNA. Translation: AAA70346.1 .
PIRi T05974.

3D structure databases

ProteinModelPortali P80284.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P80284. 1 interaction.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P80284.

Gene expression databases

Genevestigatori P80284.

Family and domain databases

Gene3Di 3.40.30.10. 3 hits.
InterProi IPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 4 hits.
TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and developmental expression of duplicated genes encoding protein disulfide isomerase in barley (Hordeum vulgare L.)."
    Chen F., Hayes P.M.
    Plant Physiol. 106:1705-1706(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Morex.
    Tissue: Ovary.
  2. "Separation of acidic barley endosperm proteins by two-dimensional electrophoresis."
    Flengsrud R.
    Electrophoresis 14:1060-1066(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-42.
    Strain: cv. H354-295-2-5.
    Tissue: Starchy endosperm.

Entry informationi

Entry nameiPDI_HORVU
AccessioniPrimary (citable) accession number: P80284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi