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Protein

Protein disulfide-isomerase

Gene

PDI

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the folding of proteins containing disulfide bonds, may be involved in glycosylation, prolyl hydroxylation and triglyceride transfer.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67NucleophileBy similarity1
Sitei68Contributes to redox potential valueBy similarity1
Sitei69Contributes to redox potential valueBy similarity1
Active sitei70NucleophileBy similarity1
Sitei135Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei411NucleophileBy similarity1
Sitei412Contributes to redox potential valueBy similarity1
Sitei413Contributes to redox potential valueBy similarity1
Active sitei414NucleophileBy similarity1
Sitei474Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase (EC:5.3.4.1)
Short name:
PDI
Alternative name(s):
Endosperm protein E-1
Gene namesi
Name:PDI
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000003420826 – 513Protein disulfide-isomeraseAdd BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi67 ↔ 70Redox-activePROSITE-ProRule annotation
Glycosylationi282N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi411 ↔ 414Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP80284

Expressioni

Gene expression databases

ExpressionAtlasiP80284 baseline and differential

Interactioni

Protein-protein interaction databases

IntActiP80284, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliP80284
SMRiP80284
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 149Thioredoxin 1PROSITE-ProRule annotationAdd BLAST124
Domaini369 – 488Thioredoxin 2PROSITE-ProRule annotationAdd BLAST120

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi510 – 513Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190 Eukaryota
COG0526 LUCA

Family and domain databases

InterProiView protein in InterPro
IPR005788 Disulphide_isomerase
IPR005792 Prot_disulphide_isomerase
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 2 hits
SUPFAMiSSF52833 SSF52833, 4 hits
TIGRFAMsiTIGR01130 ER_PDI_fam, 1 hit
TIGR01126 pdi_dom, 2 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAISKVWISL LLALAVVLSA PAARAEEAAA AEEAAAPEAV LTLHADNFDD
60 70 80 90 100
AIGQHPFILV EFYAPWCGHC KSLAPEYEKA AQLLSKHDPA IVLAKVDAND
110 120 130 140 150
EKNKPLAGKY EVQGFPTLKI FRNGGKSIQE YKGPREAEGI VEYLKKQVGP
160 170 180 190 200
ASKEIKAPED ATYLEDGKIH IVGVFTEFSG PEFTNFLEVA EKLRSYYDFG
210 220 230 240 250
HTVHANHLPR GDAAVERPVV RLFKPFDELV VDSKDFDVSA LEKFIDASST
260 270 280 290 300
PKVVIFDKNP DNHPYLLKFF QSNAPKAMLF LNFSTGPFES FKSAYYGAVE
310 320 330 340 350
EFSGKDVKFL IGDIESSQGA FQYFGLKVDQ APLILIQDGD SKKFLKEHVE
360 370 380 390 400
AGQIVAWLKD YFDGKLTPFR KSEPIPEANN EPVKVVVADN VHDVVFKSGK
410 420 430 440 450
NVLIEFYAPW CGHCKKLAPI LDEAAATLQS EEDVVIAKMD ATENDVPGEF
460 470 480 490 500
DVQGYPTLYF VTPSGKKVSY EGGRTADEIV DYIRKNKETA GQAAAATEKA
510
AEPAATEPLK DEL
Length:513
Mass (Da):56,463
Last modified:October 1, 1996 - v2
Checksum:i03BBAE2EE164F972
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33250 mRNA Translation: AAA70344.1
L33251 mRNA Translation: AAA70345.1
L33252 mRNA Translation: AAA70346.1
PIRiT05974

Similar proteinsi

Entry informationi

Entry nameiPDI_HORVU
AccessioniPrimary (citable) accession number: P80284
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1994
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

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