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P80280

- DMS4_PHYSA

UniProt

P80280 - DMS4_PHYSA

Protein

Dermaseptin-4

Gene
N/A
Organism
Phyllomedusa sauvagei (Sauvage's leaf frog)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Possesses a potent antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, protozoa, and the enveloped herpes simplex virus type 1. Probably acts by disturbing membrane functions with its amphipathic structure. Binds to healthy erythrocytes (this binding is receptor independent), and has strong hemolytic activity. Does not bind to P.falciparum infected erythrocytes, but accumulates within the parasite. Kills the parasite, and only at high concentrations has a hemolytic activity on the host cell.2 Publications

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB-KW
    2. defense response to fungus Source: UniProtKB-KW
    3. hemolysis in other organism Source: UniProtKB-KW

    Keywords - Molecular functioni

    Amphibian defense peptide, Antibiotic, Antimicrobial, Fungicide

    Keywords - Biological processi

    Cytolysis, Hemolysis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dermaseptin-4
    Alternative name(s):
    DS IV
    Dermaseptin-S4
    Short name:
    DS4
    OrganismiPhyllomedusa sauvagei (Sauvage's leaf frog)
    Taxonomic identifieri8395 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaHyloideaHylidaePhyllomedusinaePhyllomedusa

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Pharmaceutical usei

    Derivatives of this peptide may be used as therapeutic agents to treat bacterial infections and malaria, and to prevent infection by herpes simplex virus type 1 and HIV-1.

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi4 – 41M → K: K4-S4-(1-13) selectively disrupts the plasma membrane of the intracellular parasite P.falciparum without harming that of the mammalian host cell; when associated with 14-A--A-27 DEL.
    Mutagenesisi14 – 2714Missing: K4-S4-(1-13) selectively disrupts the plasma membrane of the intracellular parasite P.falciparum without harming that of the mammalian host cell; when associated with K-4. Add
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Peptidei1 – 2727Dermaseptin-4PRO_0000043642Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed by the skin glands.

    Structurei

    Secondary structure

    1
    27
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni4 – 63
    Beta strandi7 – 115

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DCXNMR-A1-13[»]
    2DD6NMR-A1-13[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP80280.

    Family & Domainsi

    Sequence similaritiesi

    Sequencei

    Sequence statusi: Complete.

    P80280-1 [UniParc]FASTAAdd to Basket

    « Hide

    ALWMTLLKKV LKAAAKALNA VLVGANA                            27
    Length:27
    Mass (Da):2,779
    Last modified:February 1, 1994 - v1
    Checksum:i43C94D2DC19721A8
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DCX NMR - A 1-13 [» ]
    2DD6 NMR - A 1-13 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P80280.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Isolation and structure of novel defensive peptides from frog skin."
      Mor A., Nicolas P.
      Eur. J. Biochem. 219:145-154(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION.
      Tissue: Skin secretion.
    2. "Selective cytotoxicity of dermaseptin S3 toward intraerythrocytic Plasmodium falciparum and the underlying molecular basis."
      Ghosh J.K., Shaool D., Guillaud P., Ciceron L., Mazier D., Kustanovich I., Shai Y., Mor A.
      J. Biol. Chem. 272:31609-31616(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    3. "Affinity driven molecular transfer from erythrocyte membrane to target cells."
      Feder R., Nehushtai R., Mor A.
      Peptides 22:1683-1690(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: POTENTIAL THERAPEUTIC USAGE.
    4. "Antibacterial properties of dermaseptin S4 derivatives with in vivo activity."
      Navon-Venezia S., Feder R., Gaidukov L., Carmeli Y., Mor A.
      Antimicrob. Agents Chemother. 46:689-694(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: POTENTIAL THERAPEUTIC USAGE IN TREATMENT OF BACTERIAL INFECTIONS.
    5. "Targeting of nonkaryophilic cell-permeable peptides into the nuclei of intact cells by covalently attached nuclear localization signals."
      Hariton-Gazal E., Feder R., Mor A., Graessmann A., Brack-Werner R., Jans D., Gilon C., Loyter A.
      Biochemistry 41:9208-9214(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: POTENTIAL THERAPEUTIC USAGE.
    6. "Direct interaction of dermaseptin S4 aminoheptanoyl derivative with intraerythrocytic malaria parasite leading to increased specific antiparasitic activity in culture."
      Efron L., Dagan A., Gaidukov L., Ginsburg H., Mor A.
      J. Biol. Chem. 277:24067-24072(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: POTENTIAL THERAPEUTIC USAGE IN TREATMENT OF MALARIA.
    7. "In vitro antiviral activity of dermaseptins against herpes simplex virus type 1."
      Belaid A., Aouni M., Khelifa R., Trabelsi A., Jemmali M., Hani K.
      J. Med. Virol. 66:229-234(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: POTENTIAL THERAPEUTIC USAGE IN PREVENTION OF HERPES SIMPLEX VIRUS TYPE 1 INFECTION.
    8. "The antimicrobial peptide dermaseptin S4 inhibits HIV-1 infectivity in vitro."
      Lorin C., Saidi H., Belaid A., Zairi A., Baleux F., Hocini H., Belec L., Hani K., Tangy F.
      Virology 334:264-275(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: POTENTIAL THERAPEUTIC USAGE IN PREVENTION OF HIV-1 INFECTION.
    9. "Physicochemical properties that enhance discriminative antibacterial activity of short dermaseptin derivatives."
      Rotem S., Radzishevsky I., Mor A.
      Antimicrob. Agents Chemother. 50:2666-2672(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: POTENTIAL THERAPEUTIC USAGE IN TREATMENT OF BACTERIAL INFECTIONS.
    10. "Consequences of N-acylation on structure and membrane binding properties of dermaseptin derivative K4-S4-(1-13)."
      Shalev D.E., Rotem S., Fish A., Mor A.
      J. Biol. Chem. 281:9432-9438(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 1-14 OF MUTANT MET-4 AND 14-ALA--ALA-27 DEL.

    Entry informationi

    Entry nameiDMS4_PHYSA
    AccessioniPrimary (citable) accession number: P80280
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1994
    Last sequence update: February 1, 1994
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Pharmaceutical

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3